Sandwich elisa

Manufactured by Aviscera Bioscience

Sandwich ELISA is a laboratory technique used for the quantitative detection and measurement of specific proteins or antigens in a sample. It involves the use of two antibodies: a capture antibody that is immobilized on a solid surface, and a detection antibody that is labeled with an enzyme. The target analyte is 'sandwiched' between the two antibodies, and the enzymatic reaction that follows allows for the quantification of the analyte.

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Lab products found in correlation

Sample buffer, by Aviscera Bioscience (2 mentions)

3 protocols using sandwich elisa

Plasma Gelsolin Quantification by ELISA

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pGSN concentrations were assayed in the study participants by sandwich ELISA (Aviscera Bioscience, Inc. CA), according to manufacturer’s instructions. The detection antibody was raised against human plasma (soluble) gelsolin. Plasma samples were diluted in a sample buffer (1/1500; Aviscera Bioscience, Inc. CA) and all analyses done in triplicate. Optical densities (OD) were determined using a microtiter plate reader at 450 nm. The blank was subtracted from the triplicate readings for each standard and sample and concentrations reported in µg/mL.

Asare-Werehene M., McGuinty M., Vranjkovic A., Galipeau Y., Cowan J., Cameron B., Cooper C.L., Langlois M.A., Crawley A.M, & Tsang B.K. (2022). Longitudinal profiles of plasma gelsolin, cytokines and antibody expression predict COVID-19 severity and hospitalization outcomes. Frontiers in Immunology, 13, 1011084.

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Quantifying pGSN in OVCA cells

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pGSN contents in cell-free conditioned media and lysates from OVCA cells were assayed by the sandwich ELISA (Aviscera Bioscience, Inc. CA), according to the manufacturer’s instructions.

Asare-Werehene M., Nakka K., Reunov A., Chiu C.T., Lee W.T., Abedini M.R., Wang P.W., Shieh D.B., Dilworth F.J., Carmona E., Le T., Mes-Masson A.M., Burger D, & Tsang B.K. (2019). The exosome-mediated autocrine and paracrine actions of plasma gelsolin in ovarian cancer chemoresistance. Oncogene, 39(7), 1600-1616.

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Pre-operative pGSN ELISA in OVCA

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Pre-operative pGSN concentrations were assayed in OVCA patients (n = 99) by sandwich ELISA (Aviscera Bioscience, Inc. CA), according to manufacturer’s instructions. The detection antibody was raised against human plasma (soluble) gelsolin. Plasma samples were diluted in a sample buffer (1/1500; Aviscera Bioscience, Inc. CA) and all analyses done in triplicate. Optical densities (OD) were determined using a microtiter plate reader at 450 nm. The blank was subtracted from the triplicate readings for each standard and sample and concentrations reported in µg/mL.

Asare-Werehene M., Communal L., Carmona E., Le T., Provencher D., Mes-Masson A.M, & Tsang B.K. (2019). Pre-operative Circulating Plasma Gelsolin Predicts Residual Disease and Detects Early Stage Ovarian Cancer. Scientific Reports, 9, 13924.

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