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Triglycerides colorimetric assay kit

Manufactured by Elabscience

The Triglycerides colorimetric assay kit is a laboratory product designed to quantitatively determine the triglyceride concentration in a sample. The kit utilizes a colorimetric method to measure the absorbance of the reaction product, which is proportional to the triglyceride concentration in the sample.

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2 protocols using triglycerides colorimetric assay kit

1

Comprehensive Biochemical Assays for Metabolic Profiling

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Colorimetric assay kits for glucose (Cat # E-BC-K234, Elabscience Biotechnology
Inc.), alkaline phosphatase activity assay kit (Cat # E-BC-K091, Elabscience
Biotechnology Inc.), and alanine aminotransferase activity assay kit (Cat #
E-BC-K235, Elabscience Biotechnology Inc.); ELISA kits for rat insulin (Cat #
E-EL-R3034, Elabscience Biotechnology Inc.), rat IL-6 (Cat # E-EL-R0015,
Elabscience Biotechnology Inc.), and TNF alpha (Cat # E-EL-R0019, Elabscience
Biotechnology Inc.). Creatinine (Cr) colorimetric assay kit (Cat # E-BC-K188-M,
Elabscience Biotechnology Inc.), high-density lipoprotein cholesterol (HDL-C)
colorimetric assay kit (Cat # E-BC-K222-S, Elabscience Biotechnology Inc.), and
triglycerides colorimetric assay kit (Cat # E-BC-K261-M, Elabscience
Biotechnology Inc.). GLP-1 assay kit (Cat # E-EL-R3007, Elabscience
Biotechnology Inc.). Readings for ELISA kits were taken using Microplate ELISA
Reader (BIOBASE-EL 10A).
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2

Adipogenic Differentiation and Muscular Fatty Infiltration

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Tissue slices or cultured cells were first fixed in 4% paraformaldehyde for 10 min, followed by oil red (Solarbio, cat#G1260) treatment for 10 min. Then samples were stained with hematoxylin for 5 min and mounted with 10% glycerol. For quantification analysis of lipid after adipogenic differentiation, the oil red was extracted by isopropanol for 5 min, and the value at 496 nm absorbance was detected by a microplate reader (Thermo Fisher Scientific).
Triglycerides colorimetric assay kit (Elabscience, cat#E-BC-K261-M) was employed to quantify the muscular fatty infiltration after tendon injury. Briefly, the muscle was first grounded with isopropanol (ACMEC, cat#I86620). Then tissue fragments were discarded by centrifugal machine at 10000 × g for 3 min. The supernatant was used to detect triglycerides by microplate reader at 510 nm absorbance.
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