Nanostructural investigation of magnetosomes was performed as described in Rosenfeldt et al. [43 (link)]. Briefly, harvested cells were centrifuged at 8300 g for 10 min using a Sorvall RC-5B Plus centrifuge (Thermo Fisher Scientific, Waltham, USA), resuspended in 50 mM HEPES buffer pH 7.0 and filled into glass capillaries (ø = 1 mm, Hilgenberg, Germany). Samples were measured using a Double Ganesha AIR system (SAXSLAB, Skovlunder, Denmark). A rotating anode (Cu, MicroMax 007HF, Rigaku Corporation, Japan) served as source for monochromatic radiation with a wavelength of λ = 1.54 Å. Two dimensional scattering patterns were recorded with a position-sensitive detector (PILATUS 300 K, Dectris) and converted into 1-dimensional intensity profiles of I(q) vs q by radial averaging. The obtained 1D-SAXS data were normalized to accumulation time, sample thickness and transmission before subtraction of the scattering contributions of the solvent. A glass capillary filled with HEPES buffer was used for background correction. The scattering curves were analyzed based on a model of monodispersed, non-interacting spheres arranged in a chain using the software SasView 4.2.
Sorvall rc 5b plus
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Sorvall RC-5B Plus is a high-speed centrifuge designed for a wide range of laboratory applications. It features a maximum speed of 20,000 RPM and a maximum RCF of 48,400 x g, providing efficient separation of samples. The centrifuge offers temperature control from -20°C to +40°C, ensuring sample integrity during the separation process. With its durable construction and user-friendly interface, the Sorvall RC-5B Plus is a reliable and versatile centrifugation solution for various laboratory settings.
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3 protocols using sorvall rc 5b plus
1
Nanostructural Analysis of Magnetosomes
2
Targeted Liposomal Delivery of Melatonin
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Two types of liposomes were prepared to target two different organs; DCP and stearylamine were used to target liver and lung tissue, respectively. Multi-lamellar liposomes were made with PE, cholesterol, DCP/stearylamine, and melatonin in the molar ratio of 7:1:1:1.13 (link) Briefly, PE, cholesterol, DCP/stearylamine, and melatonin were dissolved in a mixture of methanol and chloroform (1:2 v/v) in a round-bottomed flask. A thin dry coating was formed in the inner side of the flask on evaporating the organic solvents, and the flask was desiccated overnight. The dried layer was swollen in phosphate-buffered saline (PBS; pH 7.2) for 1 hour and then sonicated for 30 seconds with a probe type sonicator. The suspension was spun at 105,000× g in a Sorvall RC 5B Plus ultracentrifuge (Sorvall T-865 rotor; Thermo Fisher Scientific) for 1 hour, the pellet was washed twice with PBS and resuspended in 2 mL PBS.
3
PCR Detection of bla_CMY Genes
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