6410b triple quadrupole ms

The FMN photolytic reaction on TC degradation was analyzed using an LC-MS/MS method. The separation of TC and FMN in the solutions is described in Section 2.3. The samples were maintained under blue light irradiation at 20 W/m² for 1 h. TC, FMN, and D-TCF were eluted by an Agilent Poroshell 120 EC-C18 column (2.7 µm, 4.6 mm × 150 mm, Agilent Technologies, Palo Alto, CA, USA), followed by LC-MS/MS analysis. Using an Agilent 1200 Series HPLC System, an electrospray ionization (ESI) source was connected to an Agilent 6410B triple quadrupole MS in this study.
The mobile phase was composed of 0.1% methanoic acid (A) and methanol (B). The process of separation was completed by a mobile phase, the profile of which is depicted below. The linear gradient started with 0–2 min, 95%–80% A; 2–8 min, 80% A; 8–12 min, 80%–40% A; 12–15 min, 40% A; 15–19 min, 40%–5% A; 19–24 min, 5% A; and 24–27 min, 5%–95% A. The final mobile phase was set at 95% solvent A from 27 to 30 min. The reaction solution at a volume of 10 μL was injected at a flow rate of 0.4 mL per minute. TC or D-TCF was investigated by a positive-ion mode for charged fragments. The data were obtained and inspected using the MassHunter Workstation software, version B.06.00.

An Agilent 1290 series HPLC system and a 6410B triple quadrupole MS (Agilent Technologies, Inc.; Santa Clara, CA, USA) equipped with an electrospray ionization device were used for sample analysis. An Alltima C18 column (150 × 4.6 mm I.D., 5 μm) was purchased from W.R. Grace & Co. (Columbia, MD, USA).
The Alltima C18 column was operated at an oven temperature of 40°C for separation. Mobile phase A was 0.005 mol/L ammonium acetate aqueous solution containing 0.1% formic acid, and mobile phase B was methanol. Mobile phases A and B were mixed at a ratio of 60 : 40 (V/V). The flow rate and injection volume were 0.5 mL/min and 10 μL, respectively.
A triple quadrupole MS equipped with a positive electrospray ionization source was used in the MRM mode. The equipment was set with a drying gas flow, nebulizer pressure, gas temperature, and spray voltage of 10 L/min, 30 psi, 300°C, and 5500 V, respectively. A spray voltage of 5500 V was used for MS.
The MRM conditions were individually optimized for each of the six PGIs (impurities C–H) on account of their different structures, and the MS conditions for MRM are summarized in Table 1.