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Gelatin, sodium dodecyl sulfate (SDS), dithiothreitol, NaCl, EDTA, heparin, trichloroacetic acid, trypan blue, propidium iodide, RNase, cesium chloride, M199 medium, dimethyl-α-ketoglutarate, aspartate, hydrogen peroxide, glutamine, dialyzed fetal bovine serum, trypsin, 6-diazo-5-oxo-L-norleucine (DON), mercaptoethanol, streptomycin, penicillin, bis-2-(5-phenylacetomido-1,3,4-thiadiazol-2-yl)ethyl sulfide (BPTES), glutamate, ammonium chloride, and diethylamine NONOate (DEA-NO), K₂HPO₄, ATP, NAD, hydrazine, bovine liver glutamate dehydrogenase, and asparagine were from Sigma-Aldrich (St. Louis, MO). Endothelial cell growth factor was from Becton Dickinson Biosciences (Bedford, MA). Rainbow molecular weight markers were from GE Healthcare (Piscataway, NJ). Lipofectamine and 5-(and-6)-chloromethyl-2,7-dichlorodihydrofluorescein diacetate acetyl ester (CM-H₂DCFDA) were from Life Technologies Corporation (Carlsbad, CA). CB-839 and N^G-nitro-L-arginine methyl ester (L-NAME), were from Selleckchem (Houston, TX). Antibodies against GLS1, cyclin D1, cyclin E, cyclin A, p21, p27, p53, and β-actin were from Santa Cruz Biotechnology (Santa Cruz, CA), an antibody against phospho-retinoblastoma protein was from Cell Signaling Technologies (Beverley, MA), and an antibody against HO-1 was from Assay Designs (Ann Arbor, MI). [³H]Thymidine (20 Ci/mmol) was from Perkin Elmer (Boston, MA).