Anti nlrp3 ag 20b 0014

Manufactured by Adipogen

Sourced in United States

The Anti-NLRP3 (AG-20B-0014) is an antibody that specifically targets the NLRP3 protein. NLRP3 is a component of the NLRP3 inflammasome, a protein complex involved in the inflammatory response. This antibody can be used for the detection and study of NLRP3 in research applications.

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Lab products found in correlation

Nigericin, by Merck Group (5 mentions) Mitosox, by Thermo Fisher Scientific (5 mentions) Adenosine triphosphate (atp), by Merck Group (5 mentions) Anti mouse caspase 1 p20 ag 20b 0042, by Adipogen (4 mentions) Ultrapure lps, by Thermo Fisher Scientific (3 mentions) Anti mouse il 1β p17 af 401 na, by R&D Systems (3 mentions) Anti nek7 sc 50756, by Santa Cruz Biotechnology (3 mentions) Anti β actin 66009 1 ig, by Proteintech (2 mentions) Anti mouse caspase 1 ag 20b 0042, by Adipogen (2 mentions) Poly a t, by Merck Group (2 mentions) Pam3csk4, by Thermo Fisher Scientific (2 mentions) Protein g agarose, by Merck Group (2 mentions) Streptavidin coated beads, by Merck Group (2 mentions) Anti asc sc 22514 r, by Santa Cruz Biotechnology (2 mentions) Anti β actin p30002, by Abmart (2 mentions) Anti human cleaved il 1β a5208206, by Sangon (2 mentions) Anti flag f2555, by Merck Group (2 mentions) Anti human caspase 1, by Cell Signaling Technology (2 mentions) Mitotracker, by Thermo Fisher Scientific (2 mentions) Trizol reagent, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

Anti-NLRP3 (AG-20B-0014-C100) (5 citations) Mouse anti-NLRP3 (#AG-20B-0014-C100 (2 citations) Anti-mouse NLRP3 (AG-20B-0014) (3 citations) NLRP3 (AG-20B-0014) (5 citations) AG-20B-0014 (44 citations) Anti-NLRP3 (47 citations) NLRP3 (66 citations)

9 protocols using anti nlrp3 ag 20b 0014

Inflammasome Activation Assay Protocol

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HDM was from Greer Laboratories. Anti-mouse IL-1β (p17) (AF-401-NA) was from R&D. Anti-mouse caspase-1 (p20) (AG-20B-0042) and anti-NLRP3 (AG-20B-0014) antibodies were from Adipogen. Anti-ASC (67824) antibody was from Cell Signaling Technology. Anti-β-actin (66009-1-Ig) was from Proteintech.
Anti-mouse antibodies used for flow cytometry were: CD3-FITC (BD, 553062, 145-2C11), CD19-FITC (eBioscience, 11-0193-82, 1D3), Ly-6G-PE (Biolegend, 127608, 1A8), CD45-PE (eBioscience, 12-0451-81, 30-F11), CD11c-PerCP-Cy5.5 (Biolegend, 117328, N418), CD11b-PerCP-Cy5.5 (BD, 550993, M1/70), CD11b-PE-Cy7 (eBioscience, 25-0112-82, M1/70), CD3e-PE-Cy7 (BD, 552774, 145-2C11), CD19-PE-Cy7 (Biolegend, 115520, 6D5), SiglecF-Alexa Fluor 647 (BD, 562680, E50-2440), MHCII-APC-eFluor 780 (eBioscience, 47-5321-82, M5/114.15.2), Ly-6G-BV421 (Biolegend, 127628, 1A8), CD45-BV510 (Biolegend, 103137, 30-F11), CD11c-BV510 (Biolegend, 117338, N418). Ultrapure LPS was obtained from Invitrogen. Nigericin was obtained from Sigma-Aldrich. RRx-001 (S8405) was from Selleck.

Ma M., Li G., Qi M., Jiang W, & Zhou R. (2021). Inhibition of the Inflammasome Activity of NLRP3 Attenuates HDM-Induced Allergic Asthma. Frontiers in Immunology, 12, 718779.

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Investigating NLRP3 Inflammasome Activation

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Lipopolysaccharides (LPS, #L2880), adenosine triphosphate (ATP, #A3377), and aluminum potassium sulfate dodecahydrate (Alum, #237086) were purchased from Sigma (St. Louis, MO, USA). COS was bought from Abphyto Biotech Co., Ltd. (Chengdu, China). MitoSOX (#M36008) was obtained from Invitrogen (Carlsbad, CA, USA). MitoTracker (#C1049B) and protein A + G agarose (#P2012) were supplied by Beyotime (Shanghai, China). Anti-NLRP3 (#AG-20B-0014) and anti-mouse caspase-1 (#AG-20B-0042) antibodies were purchased from Adipogen (San Diego, CA, USA). Anti-NEK7 (#ab133514) and anti-GSDMD (#ab209845) antibodies were obtained from Abcam (Cambridge, UK). Anti-mouse IL-1β (#AF-401-NA) antibody was from R&D Systems (Minneapolis, MN, USA). Anti-Flag (#20543-1-AP), anti-HA (#66006-2-Ig), and anti-β-actin (#66009-1-Ig) antibodies were purchased from Proteintech (Rosemont, IL, USA).

Xu H., Chen J., Chen P., Li W., Shao J., Hong S., Wang Y., Chen L., Luo W, & Liang G. (2022). Costunolide covalently targets NACHT domain of NLRP3 to inhibit inflammasome activation and alleviate NLRP3-driven inflammatory diseases. Acta Pharmaceutica Sinica. B, 13(2), 678-693.

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Cytokine Activation Pathway Analysis

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Phorbol-12-myristate-13-acetate (PMA), MSU, ATP, nigericin, poly A:T, Pam3CSK4, A9C, DIDS, and IAA94 were purchased from Sigma. MitoSox, MitoTracker Red, DAPI, MQAE, Lipofectamine RNAiMAX, Lipofectamine 2000, and M-MLV were from Invitrogen. Ultrapure LPS was obtained from Invivogen. SYRB Green was from TransGen Biotech. MnTBAP was from Santa Cruz. Salmonella is a gift from R.V. Bruggen. Anti-human cleaved IL-1β (2021, 1:1000 dilution) and anti-human caspase-1(2225, 1:1000 dilution) antibodies were purchased from Cell Signalling. Anti-human pro-IL-1β (60136-1-lg, 1:1000 dilution) anti-CLIC1(14545-1-AP, 1:500 dilution), anti-GAPDH (10494-1-AP, 1:1000 dilution), anti-VDAC1 (66345-1-lg, 1:1000 dilution), and anti-ATP1A1 (55187-1-AP, 1:1000 dilution) antibodies were from Proteintech. Anti-CLIC4 (SC-135739, 1:1000 dilution), anti-NEK7 (SC-50756, 1:500 dilution), and anti-ASC (rabbit, SC-22514, 1:400 dilution) antibodies were from Santa Cruz. Anti-ASC (mouse, 04–147, 1:1000 dilution) antibody was from Merck Millipore. Anti-CLIC5 (ACL-025, 1:250 dilution) antibody was from Alomone labs. Anti-mouse IL-1β (AF-401-NA, 1:1000 dilution) antibody was from R&D. Anti-mouse caspase-1 (p20) (AG-20B-0042, 1:1000 dilution) and anti-NLRP3 (AG-20B-0014, 1:1000 dilution) antibodies were from Adipogen. Anti-β-actin (P30002, 1:1000 dilution) antibody was from Abmart.

Tang T., Lang X., Xu C., Wang X., Gong T., Yang Y., Cui J., Bai L., Wang J., Jiang W, & Zhou R. (2017). CLICs-dependent chloride efflux is an essential and proximal upstream event for NLRP3 inflammasome activation. Nature Communications, 8, 202.

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NLRP3 Inflammasome Activation Pathway

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MSU, nigericin, ATP, PMA, BHB, DL-sulforaphane, poly(dA:dT), and glucose were purchased from Sigma-Aldrich. MCC950 was acquired from Selleck. BAY 11-7082, isoliquiritigenin, parthenolide, flufenamic acid, and mefenamic acid were acquired from Topscience. Human recombinant insulin was bought from Novo Nordisk. The One Touch Ultra Blood glucose Test System kit was bought from Roche. Ultrapure LPS, Pam3CSK4, MitoTracker, and MitoSOX were obtained from Invitrogen. Protein G agarose and streptavidin-coated beads were respectively supplied by Millipore and Pierce Biochemicals. ATP-coupled beads were from BioWorld Company. Anti-mouse caspase-1 (p20; AG-20B-0042) and anti-NLRP3 (AG-20B-0014) were from Adipogen. Anti-ASC (sc-22514-R) and anti-NEK7 (SC-50756) were from Santa Cruz. Anti-β-actin (P30002) was bought from Abmart. Anti-human caspase-1 was from Cell Signaling Technology. Anti-human cleaved IL-1β (A5208206) was from Sangon Biotech. Anti-mouse IL-1β (p17; AF-401-NA) was from R&D Systems. Anti-Flag (F2555) and anti-Mcherry (V4888) were from Sigma-Aldrich. Recombinant human NLRP3 was from Novus Biologicals. A standard HFD (D12492, 60% kcal fat) was from Research Diet Company.

Jiang H., He H., Chen Y., Huang W., Cheng J., Ye J., Wang A., Tao J., Wang C., Liu Q., Jin T., Jiang W., Deng X, & Zhou R. (2017). Identification of a selective and direct NLRP3 inhibitor to treat inflammatory disorders. The Journal of Experimental Medicine, 214(11), 3219-3238.

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Molecular Profiling of Inflammasome Activation

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3,3′,5,5′-Tetramethylbenzidine (TMB), bovine serum albumine (BSA), ATP, protease and phosphatase inhibitors, hydrogen peroxide, bicinchoninic acid (BCA) protein assay kit, anti-β-actina (A1978-200UL) isotype mouse, and 3,3′diaminobenzidine (DAB) were purchased from Sigma Aldrich (Sant Louis, MO, USA). Goat anti-mouse antibody conjugated to horseradish peroxidase (A90-116P) was purchased from Bethyl Laboratories (Montgomery, TX, USA). Mouse IL-1β/IL-1F2 kit DuoSet ELISA was purchased from R&D Systems (Oxon, UK). Anti-caspase-1 p10 (AG-20B-0044-C100) and anti-NLRP3 (AG-20B-0014), both from mouse isotype, were purchased from Adipogen Life Sciences (San Diego, CA, USA). PVDF Membrane was purchased from Millipore (Darstadt, Germany). P2X7 receptor antagonist and A438079 were purchased from Tocris Bioscience (Bristol, UK). CK-NAC Liquiform and LDH Liquiform were purchased from Labtest (Minas Gerais, Brazil). Reagents were obtained from Merck (Darmstadt, Germany). Trizol^® Reagent and SuperScript III Reverse Transcriptase were purchased from Thermo Fisher Scientific (Waltham, MA, USA). iTaq Universal SYBR® Green Supermix was purchased from Bio-Rad (Hercules, CA, USA).

Boeno C.N., Paloschi M.V., Lopes J.A., Pires W.L., Setúbal S.D., Evangelista J.R., Soares A.M, & Zuliani J.P. (2019). Inflammasome Activation Induced by a Snake Venom Lys49-Phospholipase A2 Homologue. Toxins, 12(1), 22.

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Neuroinflammation and Dopamine Signaling

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MPTP was obtained from Sigma-Aldrich or Toronto Research Chemicals. MPP⁺, LPS, ATP, nigericin, staurosporine, propodium iodide, MCC950, dopamine and glibenclamide were purchased from Sigma-Aldrich. Mito-TEMPO was obtained from ENZO Life Sciences. MitoSOX was purchased from Invitrogen. Anti-mouse caspase-1 (AG-20B-0042) and anti-NLRP3 (AG-20B-0014) antibodies were obtained from Adipogen. Anti-mouse IL-1β antibody was obtained from R&D Systems (AF-401-NA). Anti-ASC antibody (SC-22514) and anti-phospho-ERK antibody (SC-7383) were purchased from Santa Cruz. Anti-CD45-BV421 antibody was obtained from BD Biosciences. Anti-ERK antibody (4695) and anti-phospho-JNK antibody (9251) were purchased from Cell Signaling. Anti-JNK antibody (554285) was obtained from BD pharmingen.

Lee E., Hwang I., Park S., Hong S., Hwang B., Cho Y., Son J, & Yu J.W. (2018). MPTP-driven NLRP3 inflammasome activation in microglia plays a central role in dopaminergic neurodegeneration. Cell Death and Differentiation, 26(2), 213-228.

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Immune Cell RNA Isolation and Characterization

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RNA from primary immune cells was obtained as described elsewhere [28 (link)]. RNA from a panel of different human tissues was purchased from Clontech Laboratories (Mountain View, CA). RNA from immortalized cell lines was obtained as described elsewhere [4 (link)]. The cDNA clones for human Gα_s, Gα_q and Gα₁₂ were from the cDNA Resource Center (www.cdna.org). Anti-Flag M2 (F1804) was from Millipore Sigma (Oakville, CA), Anti-IL-1β (AF-201-NA) was from R&D Systems (Cedarlane, Burlington, CA), anti-capsase-1 (AG-20B-0048) and anti-NLRP3 (AG-20B-0014) were from Adipogen (Cedarlane), anti-β-actin (MA5–15739) was from Thermo Fisher Scientific (Montreal, CA). All secondary-HRP antibodies were from Abcam (Toronto, CA)

Mercier V., Boucher G., Devost D., Bourque K., Alikashani A., Beauchamp C., Bitton A., Foisy S., Goyette P., Charron G., Hébert T.E, & Rioux J.D. (2022). IBD-associated G Protein-Coupled Receptor 65 variant compromises signalling and impairs key functions involved in inflammation. Cellular signalling, 93, 110294.

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Inflammatory Signaling Pathway Reagents

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Anti-GFP (FL; sc-8334), anti-mouse Caspase-1 (sc-514), and anti-IL-1β (sc-23460) were from Santa Cruz Biotechnology. Anti-human Caspase-1 (3688S), anti-phosphorylated-IKKα/β, anti-phosphorylated-IκBα, anti-IκBα, and anti-p-IRF3 (phosphorylated at Ser396) were from Cell Signaling Technology. Anti-LC3b and anti-Atg16L1 were from MBL. Anti-NLRP3 (AG-20B-0014) was from Adipogen. Horseradish peroxidase-anti-Flag (M2) and anti-β-actin (A1978) were from Sigma. LPS was from Sigma. ATP and nigericin were obtained from Invivogen. Ovalbumin and H₂DCF-DA were purchased from Thermo Fisher. NAC (A7250–10G) and MitoTEMPO (SML0737–5MG) were purchased from Sigma.

Zhu M., Du L., Zhao R., Wang H.Y., Zhao Y., Nie G, & Wang R.F. (2020). Cell-Penetrating Nanoparticles Activate the Inflammasome to Enhance Antibody Production by Targeting Microtubule-Associated Protein 1-Light Chain 3 for Degradation. ACS nano, 14(3), 3703-3717.

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NLRP3 Inflammasome Activation and IL-1β Regulation

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ATP, MSU, poly A:T, nigericin, and glucose were from Sigma. The human recombinant insulin was from Nova Nordisk. One Touch^® Ultra^® Blood glucose Test System was from Roche. Oridonin (s2335) was bought from Selleck. MitoSOX, ultrapure LPS, Pam3CSK4 and MitoTracker were from Invitrogen. Protein G agarose and streptavidin-coated beads were, respectively, supplied by Millipore and Pierce Biochemicals. Anti-Flag (F2555) and anti-VSV (V4888) were bought from Sigma. Anti-NLRP3 (AG-20B-0014) and anti-mouse caspase-1 (p20) (AG-20B-0042) antibodies were from Adipogen. Anti-NEK7 (SC-50756) and anti-ASC (sc-22514-R) antibodies were obtained from Santa Cruz. Anti-β-actin (P30002) was bought from Abmart. Anti-human caspase-1 was from Cell Signaling Technology. Anti-human cleaved IL-1β (A5208206) was from Sangon Biotech. Anti-mouse IL-1β (p17) (AF-401-NA) was from R&D Systems. A standard high-fat diet (D12492, 60% kcal fat) was from Research Diet Company.

He H., Jiang H., Chen Y., Ye J., Wang A., Wang C., Liu Q., Liang G., Deng X., Jiang W, & Zhou R. (2018). Oridonin is a covalent NLRP3 inhibitor with strong anti-inflammasome activity. Nature Communications, 9, 2550.

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