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Fitc rat anti mouse cd41 antibody

Manufactured by BD

The FITC rat anti-mouse CD41 antibody is a laboratory reagent used to identify and quantify CD41-positive cells, such as platelets, in mouse samples. It is conjugated with the fluorescent dye FITC, allowing for detection and analysis using flow cytometry or other fluorescence-based techniques.

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2 protocols using fitc rat anti mouse cd41 antibody

1

Platelet Depletion in Mice

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Mice were intraperitoneally administered with rat anti-CD41 mAbs (BD Biosciences, 553847) or rat IgG1 control (BD Biosciences, 553922) for seven consecutive days from 7 days after LPC injection (10 μg/mouse on the first day, followed by 5 μg/mouse on following days). Platelet depletion was confirmed by flow cytometry with an FITC rat anti-mouse CD41 antibody (1:200, BD Pharmingen, 553848) on BD FACS Verse instrument.
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2

Integrin and Platelet Receptor Expression

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Integrin αIIbβ3 expression was measured using FITC Rat Anti-Mouse CD41 antibody by flow cytometry (BD Pharmingen)31 (link),53 (link),54 (link). The mRNA expression of GPIbα and GPVI was detected by quantitative real-time PCR using primers for GPIbαː Forward primer: 5′-AGTTCATACTACCCACTGGAGCC-3′, Reverse primer: 5′-GTGGGTTTATGAGTTGGAGGC-3′; GPVI: Forward primer: 5′-AGGAGACCTTCCATCTTACCCA-3′, Reverse primer: 5′ GAGCAAAACCAAATGGAGGG-3′.
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