The largest database of trusted experimental protocols

Rabbit monoclonal anti na k atpase

Manufactured by Abcam

The Rabbit monoclonal anti-Na/K ATPase is a primary antibody that recognizes the alpha subunit of the Na/K ATPase enzyme. The Na/K ATPase is responsible for maintaining the sodium-potassium gradient across the cell membrane, which is essential for various cellular processes.

Automatically generated - may contain errors

2 protocols using rabbit monoclonal anti na k atpase

1

SARS-CoV-2 Spike Protein Deglycosylation

Check if the same lab product or an alternative is used in the 5 most similar protocols
The rabbit anti–SARS-CoV-2 S polyclonal antibody, and SARS-CoV-2 full-length S, S2, RBD, and variant proteins (293T cell expressed), were purchased from Sino Biologicals. Mouse monoclonal anti–β-actin, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and rabbit monoclonal anti-MHC II antibodies were purchased from Millipore. The rabbit monoclonal anti-Na/K ATPase was obtained from ABcam. The mouse monoclonal anti-SERCA2 and rabbit monoclonal anti-MHC I antibodies were obtained from Invitrogen. The rabbit monoclonal anti-BiP/GRP78, anti-XBP1, and anti–p-eIF2α antibodies were purchased from ABclonal. All commercial antibodies were validated for specificity by companies and us via Western blot. To obtain the deglycosylated protein, S, RBD, or S2 protein was deglycosylated in a buffer solution with PNGase F (Sigma) at 37 °C for 24 h in the dark. After deglycosylation, samples were purified and checked by Western blot (9 (link)).
+ Open protocol
+ Expand
2

Antibody Validation for Protein Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
The mouse monoclonal anti-α1 subunit antibody (clone BD24) (#MAB339) and mouse monoclonal anti-β2/3 subunit antibody (clone 62–3G1) (#05–474) were obtained from Millipore. The mouse monoclonal anti-β-actin (#A1978), mouse monoclonal anti-FLAG (clone M2) (#F1804), and rabbit polyclonal anti-glucocerebrosidase (#G4046) antibodies were obtained from Sigma Aldrich. The rabbit polyclonal anti-calnexin (#ADI-SPA-860-F) and rat monoclonal anti-Grp94 (clone 9G10) (#ADI-SPA-850-F) antibodies were purchased from Enzo Life Sciences. The goat polyclonal anti-Sel1L antibody was obtained from ThermoFisher Scientific (#PA5–18943). The rabbit polyclonal anti-HRD1 antibody (#AP2184e), rabbit polyclonal anti-BiP antibody (#AP5041C), and rabbit polyclonal anti-HA antibody (#AP1012A) were obtained from Abgent. The rabbit monoclonal anti-OS-9 (#ab109510), rabbit monoclonal anti-VCP (#ab109240), and rabbit monoclonal anti-Na, K-ATPase (#ab76020) antibodies were from Abcam. The mouse monoclonal anti-ubiquitin antibody (#3936S) was obtained from Cell Signaling.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!