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Novex nupage gel electrophoresis systems

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The Novex NuPAGE Gel Electrophoresis Systems are a line of pre-cast polyacrylamide gels designed for the separation and analysis of proteins. The systems provide a consistent and reliable platform for protein electrophoresis.

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Lab products found in correlation

Phosphatase inhibitor cocktails 2 and 3, by Merck Group (8 mentions) Complete protease inhibitor, by Roche (8 mentions) Protease and phosphatase inhibitor, by Thermo Fisher Scientific (1 mentions) Iblot gel transfer device, by Thermo Fisher Scientific (1 mentions) Ripa lysis buffer, by Cell Signaling Technology (1 mentions) Phenyl methyl sulfonyl fluoride (pmsf), by Merck Group (1 mentions) Dynabead, by Thermo Fisher Scientific (1 mentions) Protease inhibitor, by Roche (1 mentions)

16 protocols using novex nupage gel electrophoresis systems

1

Western Blot Sample Preparation

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Cells were washed with PBS and lysed with RIPA buffer supplemented with Complete Protease Inhibitor (Roche) and Phosphatase Inhibitor Cocktails II and III (Sigma). Protein quantification was performed with the BCA Protein Assay Kit (Pierce). All lysates were freshly prepared and processed with Novex NuPAGE Gel Electrophoresis Systems (Thermo Fisher Scientific) followed by western blotting.
Jin H., Wang S., Zaal E.A., Wang C., Wu H., Bosma A., Jochems F., Isima N., Jin G., Lieftink C., Beijersbergen R., Berkers C.R., Qin W, & Bernards R. (2020). A powerful drug combination strategy targeting glutamine addiction for the treatment of human liver cancer. eLife, 9, e56749.
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2

Cell Lysis and Protein Quantification

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Cells were washed with PBS and lysed in RIPA buffer supplemented with Complete Protease Inhibitor (Roche) and Phosphatase Inhibitor Cocktails II and III (Sigma). Protein quantification was performed with the BCA Protein Assay Kit (Pierce). All lysates were freshly prepared and processed with Novex NuPAGE Gel Electrophoresis Systems (Thermo Fisher Scientific) followed by Western blotting.
Schepers A., Jochems F., Lieftink C., Wang L., Pogacar Z., Leite de Oliveira R., De Conti G., Beijersbergen R.L, & Bernards R. (2021). Identification of Autophagy-Related Genes as Targets for Senescence Induction Using a Customizable CRISPR-Based Suicide Switch Screen. Molecular Cancer Research, 19(10), 1613-1621.
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3

Protein Quantification and Western Blot Analysis

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Cells were washed with PBS and lysed on ice with RIPA lysis buffer supplemented with Complete Protease Inhibitor (Roche) and Phosphatase Inhibitor Cocktails II and III (Sigma). Protein concentration was measured using the BCA Protein Assay Kit (Pierce). All lysates were then freshly prepared and processed with Novex NuPAGE Gel Electrophoresis Systems (Thermo Fisher Scientific) followed by western blotting. The antibody against α-smooth muscle actin (α-SMA) (A5228) was obtained from Sigma-Aldrich (USA) and the antibody against collagen I (14695-1-AP) was achieved from ProteinTech.
Yang C., Zhang H., Chen M., Wang S., Qian R., Zhang L., Huang X., Wang J., Liu Z., Qin W., Wang C., Hang H, & Wang H. (2022). A survey of optimal strategy for signature-based drug repositioning and an application to liver cancer. eLife, 11, e71880.
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4

Cell Lysis and Protein Quantification

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Cells were washed with PBS and lysed in RIPA buffer supplemented with Complete Protease Inhibitor (Roche) and Phosphatase Inhibitor Cocktails II and III (Sigma). Protein quantification was performed with the BCA Protein Assay Kit (Pierce). All lysates were freshly prepared and processed with Novex NuPAGE Gel Electrophoresis Systems (Thermo Fisher Scientific) followed by western blotting.
Schepers A., Jochems F., Lieftink C., Wang L., Pogacar Z., Leite de Oliveira R., De Conti G., Beijersbergen R.L, & Bernards R. (2021). Identification of Autophagy-Related Genes as Targets for Senescence Induction Using a Customizable CRISPR-Based Suicide Switch Screen. Molecular Cancer Research, 19(10), 1613-1621.
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5

Western Blot Sample Preparation

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Cells were washed with PBS and lysed with RIPA buffer supplemented with Complete Protease Inhibitor (Roche) and Phosphatase Inhibitor Cocktails II and III (Sigma). Protein quantification was performed with the BCA Protein Assay Kit (Pierce). All lysates were freshly prepared and processed with Novex NuPAGE Gel Electrophoresis Systems (Thermo Fisher Scientific) followed by western blotting.
Wang C., Vegna S., Jin H., Benedict B., Lieftink C., Ramirez C., Leite de Oliveira R., Morris B., Gadiot J., Wang W., du Chatinier A., Wang L., Gao D., Evers B., Jin G., Xue Z., Schepers A., Jochems F., Mulero Sanchez A., Mainardi S., te Riele H., Beijersbergen R.L., Qin W., Akkari L, & Bernards R. (2019). Inducing and exploiting vulnerabilities for the treatment of liver cancer. Nature, 574(7777), 268-272.
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6

Western Blot Sample Preparation

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Cells were washed with PBS and lysed with RIPA buffer supplemented with Complete Protease Inhibitor (Roche) and Phosphatase Inhibitor Cocktails II and III (Sigma). Protein quantification was performed with the BCA Protein Assay Kit (Pierce). All lysates were freshly prepared and processed with Novex NuPAGE Gel Electrophoresis Systems (Thermo Fisher Scientific) followed by western blotting.
Wang C., Vegna S., Jin H., Benedict B., Lieftink C., Ramirez C., Leite de Oliveira R., Morris B., Gadiot J., Wang W., du Chatinier A., Wang L., Gao D., Evers B., Jin G., Xue Z., Schepers A., Jochems F., Mulero Sanchez A., Mainardi S., te Riele H., Beijersbergen R.L., Qin W., Akkari L, & Bernards R. (2019). Inducing and exploiting vulnerabilities for the treatment of liver cancer. Nature, 574(7777), 268-272.
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7

Cell Lysate Preparation and Western Blot Analysis

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Cells were first seeded in normal medium without inhibitors. After 24 h, the medium was replaced with fresh medium containing the inhibitors as indicated in the text. After the drug stimulation, cells were washed with cold PBS, lysed with protein sample buffer, and processed with Novex® NuPAGE® Gel Electrophoresis Systems (Thermo Fisher Scientific). HSP90, actin, vinculin, or calreticulin were used as loading controls. The quantification of immunoblots was performed on two independent experiments using Image J and normalized to loading controls are displayed in Supplementary Fig. 16. Uncropped immunoblots presented in main figures are displayed in Supplementary Fig. 17. Uncropped immunoblots presented in Supplementary Figures are included in Source Data.
Xue Y., Morris J.L., Yang K., Fu Z., Zhu X., Johnson F., Meehan B., Witkowski L., Yasmeen A., Golenar T., Coatham M., Morin G., Monast A., Pilon V., Fiset P.O., Jung S., Gonzalez A.V., Camilleri-Broet S., Fu L., Postovit L.M., Spicer J., Gotlieb W.H., Guiot M.C., Rak J., Park M., Lockwood W., Foulkes W.D., Prudent J, & Huang S. (2021). SMARCA4/2 loss inhibits chemotherapy-induced apoptosis by restricting IP3R3-mediated Ca2+ flux to mitochondria. Nature Communications, 12, 5404.
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8

Rapid Protein Extraction and Detection

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Cells were lysed with protein sample buffer, heated at 95 °C for 5 min, and processed with Novex® NuPAGE® Gel Electrophoresis Systems (Thermo Fisher Scientific). For GLUT1 and SLC38A2 detection, unboiled protein lysates were used. HSP90 and beta-actin served as loading controls.
Zhu X., Fu Z., Chen S.Y., Ong D., Aceto G., Ho R., Steinberger J., Monast A., Pilon V., Li E., Ta M., Ching K., Adams B.N., Negri G.L., Choiniere L., Fu L., Pavlakis K., Pirrotte P., Avizonis D.Z., Trent J., Weissman B.E., Klein Geltink R.I., Morin G.B., Park M., Huntsman D.G., Foulkes W.D., Wang Y, & Huang S. (2023). Alanine supplementation exploits glutamine dependency induced by SMARCA4/2-loss. Nature Communications, 14, 2894.
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9

Cell Lysis and Protein Extraction

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Cells were washed with phosphate-buffered saline (PBS) and lysed with RIPA buffer supplemented with Complete Protease Inhibitor (Roche) and Phosphatase Inhibitor Cocktails II and III (Sigma). All lysates were freshly prepared and processed with Novex NuPAGE Gel Electrophoresis Systems (Invitrogen).
Guo Y., Wang J., Benedict B., Yang C., van Gemert F., Ma X., Gao D., Wang H., Zhang S., Lieftink C., Beijersbergen R.L., te Riele H., Qiao X., Gao Q., Sun C., Qin W., Bernards R, & Wang C. (2021). Targeting CDC7 potentiates ATR-CHK1 signaling inhibition through induction of DNA replication stress in liver cancer. Genome Medicine, 13, 166.
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10

Cell Inhibitor Stimulation Assay

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Cells were seeded in medium containing 8% FBS. After 24 h, the medium was replaced with fresh medium (8% FBS) containing the inhibitors as indicated in the text. After the drug stimulation, cells were washed with cold PBS, lysed with protein sample buffer and processed with Novex NuPAGE Gel Electrophoresis Systems (Invitrogen).
Papadakis A.I., Sun C., Knijnenburg T.A., Xue Y., Grernrum W., Hölzel M., Nijkamp W., Wessels L.F., Beijersbergen R.L., Bernards R, & Huang S. (2015). SMARCE1 suppresses EGFR expression and controls responses to MET and ALK inhibitors in lung cancer. Cell Research, 25(4), 445-458.
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