Paraffin-embedded whole-fat sections from UC and control patients (n = 4) were mounted on slides. The SP staining was detected using an anti-SP rabbit polyclonal antibody (AB1566; Millipore, Darmstadt, Germany) and the EnVision+ System HRP labeled Polymer Anti-Rabbit kit (DAKO, Carpinteria, CA). The staining was performed at the Translational Pathology Core, University of California at Los Angeles, following a standard procedure described in Millipore’s manual for the primary antibody treatment (1:100 Ab dilution, pretreatment with citrate pH 6.0, antigen retrieval).
Envision system hrp labeled polymer anti rabbit kit
Manufactured by Agilent Technologies
Sourced in Germany, Denmark
The EnVision+ System HRP labeled Polymer Anti-Rabbit kit is a laboratory equipment product designed for immunohistochemistry (IHC) and immunocytochemistry (ICC) applications. It provides a ready-to-use detection system that utilizes a horseradish peroxidase (HRP)-labeled polymer to amplify the signal from rabbit primary antibodies.
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Lab products found in correlation
2 protocols using envision system hrp labeled polymer anti rabbit kit
1
Immunohistochemical Analysis of Substance P
2
Immunohistochemical Staining of Human Skin Biopsies
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For immunohistochemical staining of human skin biopsies, formalin-fixed, paraffin-embedded sections were cut (5 μm) and stained using an EnVision+ System-HRP Labeled Polymer Anti-Rabbit Kit (Dako, Glostrup, Denmark) according to the manufacturer’s protocol. Tumor samples from mice were collected, fixed and subjected to immunofluorescence staining as described previously [28 ]. Sections were examined using Leica Application Suite X (LAS X) image acquisition software and a Leica DMC5400 fluorescence microscope (Leica Microsystems, Wetzlar, Germany). All figures were prepared using ImageJ (National Institutes of Health, Bethesda, MD) and Adobe Illustrator CC (Adobe Systems, San Jose, CA). The antibodies utilized for staining are listed in Table S2 (Additional file 2 ).
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