Antibodies against gapdh 60004 1 ig

Manufactured by Proteintech

Sourced in China, United States

Antibodies against GAPDH (60004-1-Ig) are laboratory reagents produced by Proteintech. These antibodies are designed to detect the GAPDH protein, which is a commonly used reference protein in various biological applications.

Automatically generated - may contain errors

Lab products found in correlation

Pageruler prestained protein ladder 26616, by Thermo Fisher Scientific (1 mentions) Ab86714, by Abcam (1 mentions) Ab184951, by Abcam (1 mentions) Ripa buffer high r0010, by Solarbio (1 mentions) Normal mouse igg, by Santa Cruz Biotechnology (1 mentions) Normal rabbit igg, by Santa Cruz Biotechnology (1 mentions) Cycloheximide (chx), by Merck Group (1 mentions) Polybrene, by Merck Group (1 mentions) Puromycin, by Merck Group (1 mentions) γh2ax, by Cell Signaling Technology (1 mentions) Ubiquitin, by Santa Cruz Biotechnology (1 mentions) Antibodyagainst flag tag, by Merck Group (1 mentions) Ru 486, by Abcam (1 mentions) Apoptosis necroptosis antibody sampler kit, by Cell Signaling Technology (1 mentions) Ab219800, by Abcam (1 mentions) Necrosulfonamide nsa, by Abcam (1 mentions) Acetylcysteine nac, by Selleck Chemicals (1 mentions) α sma, by Cell Signaling Technology (1 mentions) Z vad fmk, by Selleck Chemicals (1 mentions) 3 methyladenine 3 ma, by Selleck Chemicals (1 mentions)

Spelling variants of this product

GAPDH (1973 citations) GAPDH (60004-1-Ig, (129 citations) Antibodies against GAPDH (14 citations) 60004-I-Ig (9 citations) GAPDH (60004-1) (8 citations)

4 protocols using antibodies against gapdh 60004 1 ig

ICA Treatment in Cell Assays

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Reagent grade ICA (purity ≥ 98% by HPLC analysis) was obtained from Nanjing Zelang Medical Technology Corporation Ltd. (Nanjing, China) and dissolved in dimethyl sulfoxide (DMSO); the final concentration of DMSO in the media was less than 0.1% (v/v). NaN₃ (A0639) was purchased from Amresco (Solon, OH, USA). RIPA buffer (high) (R0010) and protein phosphatase inhibitor (P1260) were purchased from Solarbio Life Science (Beijing, China). A glucose oxidase assay kit (E10160) and antibodies against GSK-3β (9315), p-Ser9-GSK-3β (9323) and p-PI3K (p85) (4228) were obtained from Cell Signaling Technology (Beverly, MA, USA). Goat anti-mouse IgG-HRP (SA00001-1), goat anti-rabbit IgG-HRP (SA00001-2), and antibodies against GAPDH (60004-1-Ig), and PI3K p110α (21890-1-AP) were obtained from Proteintech Group (Wuhan, China). Antibodies against PHF1 (ab184951) and PI3K (ab86714) were obtained from Abcam (Cambridge, MA, USA). PageRuler prestained protein ladder (26616) and antibodies against p-T217 (44-744) and TAU-5 (MA5-12808) were obtained from Thermo Fisher Scientific (Waltham, MA, USA). RPMI 1640 HyClone™ cell culture medium (SH30809.01) was purchased from GE Healthcare (Chicago, IL, USA).

Zhang Y., Huang N., Lu H., Huang J., Jin H., Shi J, & Jin F. (2020). Icariin protects against sodium azide—induced neurotoxicity by activating the PI3K/Akt/GSK-3β signaling pathway. PeerJ, 8, e8955.

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Antibody Procurement for Cell Analysis

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Antibodies against DICER (#3363), β‐tubulin (#2146) and γ‐H2AX (#9718) were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibody against USP7 (#A300‐033A) was purchased from BETHYL (Montgomery, TX, USA). Antibodies against GAPDH (#60004‐1‐Ig) and β‐actin (#60008‐1‐Ig) were purchased from Proteintech Group (Rosemont, IL, USA). Antibodies against MDM2 (#sc‐965), Ubiquitin (#sc‐8017), XPF (#sc‐136 153), normal mouse IgG (#sc‐2025) and normal rabbit IgG (#sc‐2027) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibody against Flag‐tag (#F1804) was purchased from Sigma‐Aldrich (St. Louis, MO, USA). Antibody against HA‐tag (#MMS‐101R) was purchased from Covance (Berkeley, CA, USA). Antibody against 53BP1 (#NB100‐304) was purchased from Novus Biologicals (Centennial, CO, USA). ProteinG Plus/ProteinA agarose suspension (#IP05) was purchased from Calbiochem (San Diego, CA, USA). Polybrene (#H9268), cycloheximide (#C7698) and puromycin (#P8833) were purchased from Sigma‐Aldrich. Camptothecin (CPT, #S1288) was purchased from Selleck (Houston, TX, USA).

Liu X., Lu R., Yang Q., He J., Huang C., Cao Y., Zhou Z., Huang J., Li L., Chen R., Wang Y., Huang J., Xie R., Zhao X, & Yu J. (2023). USP7 reduces the level of nuclear DICER, impairing DNA damage response and promoting cancer progression. Molecular Oncology, 18(1), 170-189.

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Investigating Inflammatory and Cell Death Pathways

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Human Reactive Inflammasome Antibody Sampler Kit (#25620), Apoptosis/Necroptosis Antibody Sampler Kit (#92570), GSDMD-N terminal (#36425S) and α-SMA (#19245) were purchased from Cell Signaling Technology; human PR (ab63605), anti-Caspase-1 (ab207802) and murine GSDMD (ab219800) were obtained from Abcam. Antibodies against GAPDH (60004-1-Ig) and alpha-tubulin (11224-1-AP) were from Proteintech. Human GSDMD (PA5-30823), murine anti-PR (MA1-411), and Alexa Flour^TM488 goat anti-RABBIT lgG (H+L) (A11008) were from Invitrogen.
Pre-treatment of cells with MLKL inhibitor Necrosulfonamide (NSA) (Abcam), PI3K inhibitor 3-Methyladenine (3-MA) (Selleck), caspase inhibitor Z-VAD-FMK (Selleck), Acetylcysteine (NAC) (Selleck), IL-6 inhibitor (Selleck), and RU486 (Abcam) for 30 min before adding P4 (Sigma-Aldrich).

Wu N., Zhang X., Wang Z., Zhang X., Fang C., Li H., Zhu M., Wang Y., Liao Q., Chu T.Y, & Wang J. (2023). Progesterone prevents HGSOC by promoting precancerous cell pyroptosis via inducing fibroblast paracrine. iScience, 26(4), 106523.

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Western Blot Analysis of Cell Signaling Proteins

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The cells were lysed in RIPA buffer and centrifuged at 12 000 g for 10 min at 4 °C. The supernatants were collected for Western blotting assays. Equal amounts of proteins were subjected to 10% SDS-PAGE and then transferred to 0.45-μm pore size PVDF membranes (Millipore, Billerica, MA, USA). Membranes were blocked for 2 h with 5% skim milk in Tris buffered saline containing 0.1% Tween 20 and incubated overnight at 4 °C with anti-mouse GAPDH (Cell Signaling Technology, USA), anti-rabbit LMNB2 (Abcam, USA), and anti-rabbit P21 (Cell Signaling Technology, USA). The membranes were washed 10 min three times with Tris-buffered saline containing 0.1% Tween 20 and incubated for 2h with appropriate secondary antibodies. Speci c primary antibodies against LMNB2 (ab151735) and Ki67 (ab16667) were purchased from Abcam. Antibodies against p21 (#2947), p27 (#3686), cyclin D1 (#2978), cyclin E2 (#4132), and Cdk2 (#2546) were obtained from Cell Signaling Technology. Antibodies against GAPDH (60004-1-Ig) were purchased from Proteintech.

Dong C., Jiang T., Yin H., Hu S., Zhang Y., Geng H., Shi P., Xu Y., Gao H., Liu L., Zhou L., Zhang Z., & Song J. (2020). LMNB2 Promotes the Progression of Colorectal Cancer by Silencing p21 Expression.

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