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Polymer detection system

Manufactured by Agilent Technologies

The Polymer Detection System is a laboratory instrument designed to analyze and characterize polymer samples. It provides reliable and accurate measurements of various polymer properties, such as molecular weight distribution, polydispersity, and intrinsic viscosity. The system utilizes advanced chromatographic techniques and specialized detectors to deliver comprehensive polymer characterization data.

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2 protocols using polymer detection system

1

Immunohistochemical Evaluation of MMR Proteins

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Immunohistochemical staining for MMR proteins was performed, using a primary antibody to MLH1 (clone G168–15; Biocare Medical, Concord, CA), MSH2 (clone FE11; Biocare Medical), MSH6 (clone BC/44; Biocare Medical), or PMS2 (clone A16–4; Biocare Medical), and a polymer detection system (Dako, Carpinteria, CA) on an automated staining system (Dako), on the sections (5 μm thick) from the prostate TMA, as described previously.[16 (link)] All stains were quantified independently by 2 pathologists (MS and HM) who were blinded to sample identity. Convincing nuclear staining of each protein in at least 1% of tumor cells was considered to be positive. Cases with discrepancies in the positivity were re-reviewed simultaneously by the 2 pathologists until a consensus was reached.
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2

Standardized Immunohistochemistry Analysis

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IHC was performed using either Ventana or the DAKO automated staining systems (Ventana Ultraview or the DAKO Biocare Polymer Detection System, respectively) using standardized protocols. (Details on IHC technique can be found in Supplementary Table 1). Interpretation and scoring of IHC results was done by a single pathologist (RA) in a blinded fashion. Visual semi quantitative staining was performed for markers with cytoplasmic localization (BCAT, survivin, COX-2, BRAF). Visual scores were performed using an intensity scale of 0 (negative) to 3 + and percentage of cells staining (0–100%). Quantitative automated cellular imaging system (ACIS, DAKO, Carpenteria, CA) scores for staining intensity and percentage of cells staining were generated for markers with predominant nuclear expression (p53, MGMT, p21, DNA Pkcs, p27, MSH2).
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