Membrane-induced CHMP7 polymers were prepared for negative stain EM and imaged with a Tecnai T20 microscope (FEI) with a LaB6 filament, operated at 200 kV. 227 micrographs were collected with a TemCam-F816 8k × 8k camera (TVIPS) using SerialEM software35 (link), with a nominal pixel size of 1.57 Å. The defocus was 0.7 −1.7 μm and the total dose was 20 e-/Å2 (link). Particles containing a repeating polymeric unit were picked manually along the polymeric protein chain, yielding 6094 particles. Specifically, particles were picked from polymers detached from membrane, which were in a favorable orientation for subsequent classification. Particles were picked and 2D-classified using default parameters within RELION version 2.0 software.
Temcam f816 8k 8k camera
Manufactured by TVIPS
The TemCam-F816 is an 8k × 8k camera designed for transmission electron microscopy (TEM) applications. It features a large active sensor area and high resolution, providing detailed imaging capabilities for TEM users.
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Lab products found in correlation
2 protocols using temcam f816 8k 8k camera
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Electron Microscopy Analysis of CHMP7 Polymers
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Electron Microscopy Analysis of CHMP7 Polymers
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Membrane-induced CHMP7 polymers were prepared for negative stain EM and imaged with a Tecnai T20 microscope (FEI) with a LaB6 filament, operated at 200 kV. 227 micrographs were collected with a TemCam-F816 8k × 8k camera (TVIPS) using SerialEM software35 (link), with a nominal pixel size of 1.57 Å. The defocus was 0.7 −1.7 μm and the total dose was 20 e-/Å2 (link). Particles containing a repeating polymeric unit were picked manually along the polymeric protein chain, yielding 6094 particles. Specifically, particles were picked from polymers detached from membrane, which were in a favorable orientation for subsequent classification. Particles were picked and 2D-classified using default parameters within RELION version 2.0 software.
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