TNF-α was purchased from Peprotech (Rocky Hill, NJ, USA). MTT and lithium chloride (LiCl) was bought from Sigma-Aldrich (St. Louis, MO, USA). XAV939 was got from MedChem Express (Newark, NJ, USA). Tetramethyl rhodamine methyl ester (TMRM) was bought from BestBio Company (Shanghai, China). Propidium iodide (PI) and fluorescent phalloidin were purchased from Beyotime Biotechnology (Haimen, Jiangsu, China). Transwell filter and Annexin V-PE/7-ADD kit were bought from BD Biosciences (USA). Fetal bovine serum (FBS) and Dulbecco’s modified Eagle’s medium (DMEM) were purchased from Gibco (Carlsbad, CA, USA). Antibodies of AQP1 (ab9566), Bax (ab32503), Bcl-2 (ab182858), cleaved caspase 3 (ab2302), c-myc (ab32072), MMP-2 (ab92536), MMP-9 (ab76003), Wnt1 (ab15251) and β-catenin (ab32572) were bought from Abcam (Cambridge, UK). Antibodies of cyclin D1 (#55506), GSK-3β (#12456) and p-GSK-3β (Ser9) (#9323) were purchased from Cell Signaling Technology (Beverly, MA, USA).
P gsk 3β ser9 9323
Manufactured by Cell Signaling Technology
Sourced in United States
P-GSK-3β (Ser9) (#9323) is a primary antibody that detects endogenous levels of GSK-3β only when phosphorylated at serine 9. It is intended for use in Western blotting applications.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Tnf α, by Thermo Fisher Scientific (1 mentions)
Transwell filter, by BD (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions)
Propidium iodide, by Beyotime (1 mentions)
Xav 939, by MedChemExpress (1 mentions)
P erk thr202 tyr204, by Cell Signaling Technology (1 mentions)
P chk1 ser280, by Abcam (1 mentions)
P akt ser473, by Cell Signaling Technology (1 mentions)
Mln4924, by Apexbio (1 mentions)
Aicar, by Selleck Chemicals (1 mentions)
P ampk thr172, by Cell Signaling Technology (1 mentions)
Ucn 01, by Enzo Life Sciences (1 mentions)
Compound c, by Selleck Chemicals (1 mentions)
Ly294002, by Merck Group (1 mentions)
β actin, by Merck Group (1 mentions)
2 protocols using p gsk 3β ser9 9323
1
Investigating Wnt/β-Catenin Pathway in Cell Apoptosis
2
Antibody and Compound Sources for Signaling Research
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Antibodies were obtained from commercial sources: β‐Actin (A5441), HA (11867423001), FLAG (F1804 and F7425 from Sigma, St. Louis, MO, USA), CHK1 (SC‐8408 from Santa Cruz Biotechnology, Santa Cruz, CA, USA), CUL1 (SC‐11384 from Santa Cruz Biotechnology), NEDD8 (ab81264 from Abcam, Cambridge, MA, USA), p‐CHK1 Ser280 (2347), CHK2 (2662), p‐AMPK Thr172 (2535), AMPK (D5A2), p‐AKT Ser473 (4060), AKT (4691), p‐ERK Thr202/Tyr204 (9101), ERK (4696), p‐GSK3β Ser9 (9323), GSK3α/β (5676), p‐ACC Ser79 (3661), ACC (3676), PARP (9542), β‐TrCP1 (4394), MEK1/2 (9216), and Vimentin (3390) (Cell Signaling Technology, Danvers, MA, USA). Compounds were obtained from commercial sources: MLN4924 (B1036 from Apexbio, Houston, TX, USA), Compound C (S7840 from Selleckchem, Houston, TX, USA), AICAR (S1802 from Selleckchem), UCN‐01 (ALX‐380‐222‐MC25 from Enzo Biochem), and LY294002 (L9908 from Sigma).
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