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Rabbit anti atg101

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Rabbit anti-Atg101 is a primary antibody that recognizes the autophagy-related protein Atg101. It is used in various research applications to detect and study the Atg101 protein, which plays a crucial role in the autophagy process.

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Lab products found in correlation

Rabbit anti ha, by Merck Group (2 mentions) Rabbit anti ulk1, by Merck Group (2 mentions) Nupage 4 12 bis tris precast gel, by Thermo Fisher Scientific (2 mentions) Rabbit anti phospho erk p42 p44, by Cell Signaling Technology (2 mentions) Rabbit anti akt, by Thermo Fisher Scientific (2 mentions) Rabbit anti phospho akt t308, by Thermo Fisher Scientific (2 mentions) Rabbit anti phospho akt s473, by Thermo Fisher Scientific (2 mentions) Rabbit anti phospho sgk1 ser78, by Thermo Fisher Scientific (2 mentions) Anti phospho sgk1 thr256, by Thermo Fisher Scientific (2 mentions) Rabbit anti cx37, by Abcam (2 mentions) Rabbit anti lc3a b, by Abcam (2 mentions) Rabbit anti rb1cc1, by Merck Group (2 mentions) Rabbit anti atg13, by Merck Group (2 mentions) Mouse anti erk, by Cell Signaling Technology (2 mentions) Rabbit anti phospho ulk1, by Cell Signaling Technology (1 mentions) Mab9521, by R&D Systems (1 mentions) Rabbit anti ulk1, by Cell Signaling Technology (1 mentions) 0231 100 lc3 5f10, by Nanotools (1 mentions) Rabbit anti atg13, by Cell Signaling Technology (1 mentions) Rabbit anti p62, by Cell Signaling Technology (1 mentions)

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Rabbit anti-

3 protocols using rabbit anti atg101

1

Western Blot Analysis of Protein Expression

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Protein was isolated from passage 5 HUVECs 18 h after induction of gene expression in 1% SDS lysis buffer containing protease and phosphatase inhibitor. 10 or 20 μg of protein was run on a NuPAGE 4%–12% precast Bis-Tris gel (Invitrogen) and transferred onto nitrocellulose membranes using a semi-dry (Bio-Rad) or a wet transfer method. Anti-human antibodies used are as follows: rabbit anti-phospho-ERK p42/p44 (Cell Signaling Technologies [CST] 9106) 1:1,000, mouse anti-ERK (CST 9102) 1:1,000, rabbit anti-Akt (CST 9272) 1:1,000, rabbit anti-phospho-Akt-T308 (CST 4056), rabbit anti-phospho-Akt-S473 (CST 4051), rabbit anti-serum/glucocorticoid-regulated kinase 1 (SGK1) (CST 12103) 1:1,000, rabbit anti-phospho-SGK1-Ser78 (CST 5599) 1:1,000, anti-phospho-SGK1-Thr256 (Thermo Fisher 44–1260G) 1:1,000, rabbit anti-Cx37 (Abcam 181701) 1:3,000, rabbit anti-LC3A/B (CST 4108) 1:1,000, rabbit anti-ULK1 (CST 8054) 1:1,000, rabbit anti-Atg13 (CST 13273) 1:1,000, rabbit anti-Atg101 (CST 13492) 1:1,000, rabbit anti-RB1CC1 (CST 12436) 1:1,000, rabbit anti-HA (CST 3724) 1:1,000, Actin (Sigma Aldrich A1978) 1:4,000. The following secondary antibodies were used: anti-mouse HRP-linked antibody (CST 7076) 1:2,500 or anti-rabbit HRP-linked antibody (CST 7074) 1:2,500. Quantification of western blots was performed using ImageJ (v1.51).
Ugwu N., Atzmony L., Ellis K.T., Panse G., Jain D., Ko C.J., Nassiri N, & Choate K.A. (2021). Cutaneous and hepatic vascular lesions due to a recurrent somatic GJA4 mutation reveal a pathway for vascular malformation. Human Genetics and Genomics Advances, 2(2), 100028.
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2

Western Blot Analysis of Protein Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein was isolated from passage 5 HUVECs 18 h after induction of gene expression in 1% SDS lysis buffer containing protease and phosphatase inhibitor. 10 or 20 μg of protein was run on a NuPAGE 4%–12% precast Bis-Tris gel (Invitrogen) and transferred onto nitrocellulose membranes using a semi-dry (Bio-Rad) or a wet transfer method. Anti-human antibodies used are as follows: rabbit anti-phospho-ERK p42/p44 (Cell Signaling Technologies [CST] 9106) 1:1,000, mouse anti-ERK (CST 9102) 1:1,000, rabbit anti-Akt (CST 9272) 1:1,000, rabbit anti-phospho-Akt-T308 (CST 4056), rabbit anti-phospho-Akt-S473 (CST 4051), rabbit anti-serum/glucocorticoid-regulated kinase 1 (SGK1) (CST 12103) 1:1,000, rabbit anti-phospho-SGK1-Ser78 (CST 5599) 1:1,000, anti-phospho-SGK1-Thr256 (Thermo Fisher 44–1260G) 1:1,000, rabbit anti-Cx37 (Abcam 181701) 1:3,000, rabbit anti-LC3A/B (CST 4108) 1:1,000, rabbit anti-ULK1 (CST 8054) 1:1,000, rabbit anti-Atg13 (CST 13273) 1:1,000, rabbit anti-Atg101 (CST 13492) 1:1,000, rabbit anti-RB1CC1 (CST 12436) 1:1,000, rabbit anti-HA (CST 3724) 1:1,000, Actin (Sigma Aldrich A1978) 1:4,000. The following secondary antibodies were used: anti-mouse HRP-linked antibody (CST 7076) 1:2,500 or anti-rabbit HRP-linked antibody (CST 7074) 1:2,500. Quantification of western blots was performed using ImageJ (v1.51).
Ugwu N., Atzmony L., Ellis K.T., Panse G., Jain D., Ko C.J., Nassiri N, & Choate K.A. (2021). Cutaneous and hepatic vascular lesions due to a recurrent somatic GJA4 mutation reveal a pathway for vascular malformation. Human Genetics and Genomics Advances, 2(2), 100028.
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3

Comprehensive Autophagy Protein Analysis

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The following antibodies were used: rabbit anti-C9ORF72 (sc-138763, Santa Cruz Biotechnology), rabbit anti-SMCR8 (ab121682, Abcam), rabbit anti-WDR41 (sc-137922, Santa Cruz Biotechnology), rabbit anti-ATG101 (SAB4200175, Sigma), rabbit anti-ATG13 (13468, Cell Signaling), rabbit anti-ULK1 (8054, Cell Signaling), rabbit anti–phospho-ULK1 (6888, Cell Signaling), rabbit anti-p62 (5114, Cell Signaling), rabbit anti-RFP (R10367, Invitrogen), mouse anti-LC3 (0231-100/LC3-5F10, Nanotools), rabbit anti-LC3 (PM036, MBL), mouse anti–cathepsin D (AF1029, R&D Systems), rat anti–cathepsin L (MAB9521, R&D Systems).
Yang M., Liang C., Swaminathan K., Herrlinger S., Lai F., Shiekhattar R, & Chen J.F. (2016). A C9ORF72/SMCR8-containing complex regulates ULK1 and plays a dual role in autophagy. Science Advances, 2(9), e1601167.
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