Avance drx 300

To find each compound’s melting point, an electrothermal instrument, a 9,100, was employed. ¹HNMR spectra were collected using Bruker DRX-300 Avance equipment with DMSO-d₆. Materials and reagents were acquired from Acros, Merck, and Fluka and utilized right away.

The melting points of the diverse compounds were ascertained utilizing an electrothermal apparatus denoted as the 9100. The acquisition of ¹HNMR spectra using DMSO-d₆ was accomplished through the usage of the Bruker DRX-300 Avance instruments. The aforementioned compounds were generously supplied in significant amounts by Fluka, Merck, and Acros, and were promptly utilized.

All commercially available chemical compounds and other solvents in this study were purchased from Merck and Aldrich chemical companies. The NMR spectra for synthesized compounds were acquired using a Bruker DRX-300 AVANCE instrument (300 MHz for ¹H and 75.4 MHz for ¹³C) with DMSO-d₆ as the deuterated solvent. Chemical shifts were reported in ppm (δ) relative to the internal TMS, and the coupling constant (J) given in hertz (Hz). FT-IR spectra and melting points of all the compounds were measured with a Bruker Tensor 27 spectrometer and electrothermal 9100 apparatus, respectively. Mass spectra were given by an Agilent 5975C VL MSD with a Triple-Axis detector operating at an ionization potential of 70 eV.

The melting points of the various compounds were determined using a 9100 electrothermal apparatus. The Bruker DRX-400 and DRX-300 Avance instruments were employed for the acquisition of ¹HNMR spectra utilizing DMSO-d₆. The aforementioned substances were graciously provided in substantial quantities by Fluka, Merck, and Acros, and were instantaneously employed.

Gas chromatography and mass spectrometry (GC/MS) were conducted at the Technological Development Park of the Federal University using a GC/MS QP 5050A (Shimadzu, Kyoto, Japan) and an Agilent DB-5ms nonpolar capillary column (50 m × 0.25 mm × 0.25 μm). The oven temperature was programmed at 70°C with an increase of 4°C/min until 280°C was reached and then maintained for 15 min. The carrier gas was helium, with a constant flow rate of 1.4 mL/min. The temperature of the ionization source was maintained at 280°C, ionization energy at 70 eV, and ionization current at 0.7 kV. Mass spectra were recorded from 30 to 450 m/z. Individual components were identified by matching their 70 eV mass spectra with those of the spectrometer database by using the Wiley L-Built library and by comparing their retention indices and fragmentation patterns with those of the NIST [14 ] MS library and those reported in the literature [15 ], respectively. The retention indices were compared with those obtained by Craveiro et al. [16 (link)] for other Euphorbiaceae species and simulated using the method described by Alencar et al. [17 ]. ¹H and ¹³C NMR were recorded on a Bruker Avance DRX-300 (300 MHz for ¹H and 75 MHz for ¹³C); chemical shifts are given in ppm relative to residual CHCl₃ (7.27) and to the central peak of the triplet related to CDCl₃ carbon (77.2 ppm).

Reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA) or Merck (Darmstadt, Germany). The commercially available (±)-naringenin 5 (Sigma-Aldrich) was used. The purity of the synthesized products (>95%) was established by HPLC chromatography. Analysis were performed according to the procedure described earlier [33 (link)]. The NMR spectra (¹H-, ¹³C-NMR, ¹³C-DEPT, and correlation spectra: ¹H-¹H-COSY, ¹H-¹³C-HMQC, ¹H-¹³C-HMBC) were recorded in a CDCl₃ solution on a Brüker Avance DRX 300 and Brüker Avance II 600 MHz spectrometers (Brüker, Karlsruhe, Germany). IR spectra (KBr discs) were determined on a Thermo-Nicolet IR300 FT-IR-spectrometer (Madison, WI, USA). UV spectra were run on a Visible Spectrofotometer Cintra 303, GBC, in methanol. Melting points (uncorrected) were determined on a Boetius apparatus. Bioscreen C (Lab system Oy, Helsinki, Finland) has been used in the initial part of the investigations of antimicrobial activity.