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Qcm d sensors

Manufactured by Biolin Scientific
Sourced in Sweden

QCM-D sensors are a type of quartz crystal microbalance (QCM) that can detect changes in the mass and viscoelastic properties of thin films on surfaces. They operate by measuring the changes in frequency and dissipation of an oscillating quartz crystal when a sample is placed on the sensor surface.

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2 protocols using qcm d sensors

1

Synthesis and Characterization of Hydrogels

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Monomeric acrylamide (AM), metharoycholine (DMC), and itaconic acid (IA) were obtained from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China), Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan), and Tianjin Chemical Reagent Co., Ltd. (Tianjin, China), respectively. Microcrystalline cellulose (MCC) and sodium bisulfite were purchased from Sinopharm Chemical Reagent Co., Ltd., too. Polyethyleneimine (PEI, with Mw ~ 750,000, 50 wt.% in H2O) was purchased from Sigma-Aldrich Co. LLC (Shanghai, China). The rest of the chemicals were ordered form Nanjing Chemical Reagent Co., Ltd. (Nanjing, China). All chemicals are analytical grade and used without further purification. QCM-D sensors were AT cut quartz crystals and were supplied by Biolin Scientific Co., Ltd. (Gothenburg, Sweden) with silica surface.
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2

Biofunctionalized Surface Characterization

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“Scan-Asyst
air” AFM tips were purchased from Bruker,
and the Maleimide-PEG-NHS molecule was purchased from Polypure. QCMD
sensors were purchased from Biolin Scientific. A number of peptides
were custom-ordered from PepScan (see Table 1), the reported purity is from HPLC analysis,
reported mass is the experimental mass from mass spectrometry. Peptides
were ion-exchanged to Na+ counterions by the manufacturer.
Poly(l-lysine)-grafted poly(ethylene glycol) was purchased
from SuSoS (Switzerland) with a grafting ratio of 3.5. The molecular
weight of the PLL backbone and PEG side chains was 20 and 2 kDa, respectively.
All other chemical reagents were purchased from Sigma-Aldrich.
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