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Dimerx h 2kb ig fusion protein loaded with ova257 264 peptide

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DimerX H-2Kb:Ig fusion protein loaded with OVA257–264 peptide is a laboratory reagent composed of an H-2Kb major histocompatibility complex (MHC) class I molecule fused to an immunoglobulin Fc domain, which is loaded with a specific peptide sequence derived from ovalbumin (OVA257–264). This fusion protein is designed for use in immunological research applications.

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Lab products found in correlation

Murine ril 12, by R&D Systems (2 mentions) Recombinant b7 1 fc chimeric protein, by R&D Systems (2 mentions)

2 protocols using dimerx h 2kb ig fusion protein loaded with ova257 264 peptide

1

In Vitro Activation of OT-I T Cells

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Naïve OT-I T cells were also purified as described above and stimulated for three days in vitro in flat-bottom, microtiter wells coated with antigen (DimerX H-2Kb:Ig fusion protein loaded with OVA257–264 peptide) (BD Bioscience, San Jose, CA), and recombinant B7-1/Fc chimeric protein (R&D Systems, Minneapolis, MN) as previously described22 (link). 3 × 105 cells in 1.5 mL Allos media were deposited in each well and 2.5 U/mL of IL-2 was added to wells in a 24-well plate. Where indicated, cultures were supplemented with 2 U/mL of murine rIL-12 (R&D Systems). Supernatant was harvested at the end of day three.
Li L., Jay S.M., Wang Y., Wu S.W, & Xiao Z. (2017). IL-12 stimulates CTLs to secrete exosomes capable of activating bystander CD8+ T cells. Scientific Reports, 7, 13365.
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2

OT-I CD8 Cell Activation and Rapamycin Treatment

Cited 2 times
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Naïve OT-I CD8 cells were purified as described above and stimulated for 3 days in vitro in flat-bottom microtiter wells stimulated with antigen (DimerX H-2Kb:Ig fusion protein loaded with OVA257-264 peptide; BD Pharmingen), recombinant B7-1/Fc chimeric protein (R&D Systems) and 2U/ml of murine rIL-12 (R&D Systems) as previously described (Li et al. 2011 (link); Xiao et al. 2009 (link)). 3 × 105 cells in 1.5 ml Allos media were placed in each well and 2.5U/ml IL-2 added to all wells (24-well plate). For rapamycin treated CTLs, cultures were supplemented with rapamycin at 250 ng/ml. Rapamycin was purchased from EMD (Gibbstown, NJ).
Mattson E., Xu L., Li L., Liu G.E, & Xiao Z. (2014). Transcriptome Profiling of CTLs Regulated by Rapamycin Using RNA-Seq. Immunogenetics, 66(11), 625-633.
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