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Calcein

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Calcein is a fluorescent dye that is commonly used as a metal indicator and chelating agent in biochemical and cell biology applications. It exhibits bright green fluorescence when bound to certain metal ions, making it a useful tool for various laboratory techniques.

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Lab products found in correlation

Tetracycline, by MedChemExpress (1 mentions) Ammonium ferrous sulfate hexahydrate, by Sinopharm (1 mentions) Calf thymus dna ct dna, by Merck Group (1 mentions) Calcein am, by MedChemExpress (1 mentions) 5 5 dimethyl 1 pyrroline n oxide, by Dojindo Laboratories (1 mentions) Ascorbic acid, by Merck Group (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Ferric chloride, by Sinopharm (1 mentions) Trisodium citrate dihydrate, by Sinopharm (1 mentions)

2 protocols using calcein

1

Tetracycline and Calcein Labeling for Bone Histomorphometry

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The mice were subcutaneously injected with 20 mg/kg tetracycline (MedChemExpress) or 8 mg/kg calcein (MedChemExpress) on days 3 and 13 days before being euthanized, respectively.16 (link) Mice were anesthetized and euthanized. The femurs were separated and fixed in 75% ethanol for 48 h. Then, hard tissue embedding and ultra-thin sections (100–200 μm) were generated in the dark. Cortical bone sites corresponding to the primary bone trabeculae under the femoral growth plate were observed under fluorescence microscopy (BX-53; Olympus Corporation, Tokyo, Japan), and the images were collected.
Rong X., Kou Y., Zhang Y., Yang P., Tang R., Liu H, & Li M. (2022). ED-71 Prevents Glucocorticoid-Induced Osteoporosis by Regulating Osteoblast Differentiation via Notch and Wnt/β-Catenin Pathways. Drug Design, Development and Therapy, 16, 3929-3946.
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2

Antioxidant Assays with CAPE, Caffeic Acid, and DNA

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CAPE (97%), CA (98%), FA (98%), EF (98%), caffeic acid methyl ester (CAME) (98%), caffeic acid ethyl ester (CAEE) (98%), calf thymus DNA (ct-DNA), ascorbic acid, and bovine serum albumin (BSA) were purchased from Sigma (St. Louis, MO, USA). Ferric chloride (≥99%), ferrous ammonium sulfate hexahydrate (≥99.5%), and trisodium citrate dihydrate (≥99.5%) were purchased from Sinopharm Chemical Reagent Co., Ltd. Calcein and Calcein-AM were purchased from MedChemExpress (MCE). 5,5-dimethyl-1-pyrroline N-oxide (DMPO) was purchased from DOJINDO Molecular Technologies.
Shao B., Mao L., Tang M., Yan Z.Y., Shao J., Huang C.H., Sheng Z.G, & Zhu B.Z. (2021). Caffeic Acid Phenyl Ester (CAPE) Protects against Iron-Mediated Cellular DNA Damage through Its Strong Iron-Binding Ability and High Lipophilicity. Antioxidants, 10(5), 798.
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