AacCRISPR/Cas12b protein (C2c1) and universal isothermal amplification kits were obtained from HuiDeXin Biotech Co., Ltd. (Tianjin, China). Gold nanoparticle-based lateral flow biosensor (AuNP-LFB) materials, including crimson red dye streptavidin-coated AuNP (SA-AuNP, 40 ± 5 nm), were purchased from Bangs Laboratories, Inc. (Fishers, IN), rabbit anti-fluorescein antibody (anti-FAM), and biotinylated bovine serum albumin (biotin-BSA) were obtained from Abcam Co., Ltd. (Shanghai, China). Four AuNP-LFB sections, including the sample pad, conjugation pad, nitrocellulose membranes, and absorption pad, were manufactured and laminated on plastic adhesive backing by HuiDeXing Biotech Co., Ltd., according to our design scheme (Fig. 3 ).
Rabbit anti fluorescein antibody anti fam
Manufactured by Abcam
Sourced in China
The Rabbit anti-fluorescein antibody (anti-FAM) is a laboratory reagent used to detect and quantify the presence of fluorescein in various applications. It specifically binds to the fluorescein molecule, allowing for its identification and measurement.
Automatically generated - may contain errors
Lab products found in correlation
Biotinylated bovine serum albumin biotin bsa, by Abcam (5 mentions)
Nucleic acid releasing agents, by Sansure Biotech (3 mentions)
Biotin bsa, by Abcam (3 mentions)
Dye crimson red streptavidin coated polymer nanoparticles, by Bangs Laboratories (2 mentions)
Nitrocellulose nc membrane, by Merck Group (1 mentions)
Qiaamp dna mini kit, by Qiagen (1 mentions)
Biotinylated bovine serum albumin, by Abcam (1 mentions)
Qiaamp viral rna mini kit, by Qiagen (1 mentions)
9 protocols using rabbit anti fluorescein antibody anti fam
1
Rapid CRISPR/Cas12b Lateral Flow Assay
2
Lateral Flow Assay with AuNPs Detection
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AuNPs-LFA-related materials, including crimson red dye streptavidin-coated AuNPs (SA-AuNPs; 40 ± 5 nm, 10 mg/mL), were obtained from Bangs Laboratories Inc. (Fishers, IN, USA), rabbit anti-fluorescein antibody (anti-FAM; 0.2 mg/mL) and biotinylated bovine serum albumin (biotin-BSA; 4 mg/mL) were obtained from Abcam Co., Ltd. (Shanghai, China), and nitrocellulose membrane (NC) was obtained from Merck Millipore Co., Ltd. (Darmstadt, Germany). Four components of the AuNPs-LFA, including the sample, conjugate, absorption pads, and nitrocellulose membranes, were manufactured and laminated on plastic adhesive backing by HuiDeXin Biotech. Co., Ltd. (Tianjin, China) according to our design scheme (Figure 2 ). Nucleic acid releasing agents were obtained from GenDx Biotech Co., Ltd. (Suzhou, China). LAMP amplification kits and colorimetric indicator (Leuco-hydroxynaphthol blue, L-HNB) were obtained from HaiGene Biotech Co., Ltd. (Harbin, China). Commercial RT–qPCR diagnostic kits for HIV-1 were obtained from DaAn Gene Co., Ltd. (Guangzhou, China).
3
Rapid SARS-CoV-2 Detection Assay
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Nucleic acid releasing agents were obtained from Sansure Biotech Inc. (Changsha, China). Thayer–Martin (TM) chocolate agar plates were purchased from Autobio Biotechnology Co., Ltd. (Zhengzhou, China). The colorimetric indicator, malachite green (MG) and universal isothermal amplification kits were obtained from HuiDeXin Bio-Technique (Tianjin, China). Biotinylated bovine serum albumin (Biotin-BSA) and rabbit anti-fluorescein antibody (anti-FAM) were purchased from Abcam Co., Ltd. (Shanghai, China). Streptavidin dye-coated gold nanoparticles (Crimson red) were obtained from Bangs Laboratories, Inc. (IN, United States). Gold nanoparticle-based LFB materials, including sample pad, conjugate pad, absorbent pad, nitrocellulose membranes, and backing cards were manufactured by HuiDeXing Biotech Co., Ltd. (Tianjing, China) according to our design specifications. Gonococcus commercial PCR diagnosis kits were obtained from DaAn Gene Co., Ltd. (Guangzhou, China).
4
Lateral Flow Assay for LAMP Detection
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The LAMP kits and visual detection reagents (Malachite Green, MG) were obtained from HuiDeXin Bio-technology (Tianjin, China). Nucleic acid-releasing agents were purchased from Sansure Biotech Inc. (Changsha, China). Dye streptavidin-coated gold nanoparticles (size: 35 ± 5 nm; crimson red) were obtained from Bangs Laboratories, Inc. (Indiana, USA). Rabbit anti-fluorescein antibody (anti-FAM) and biotinylated bovine serum albumin (biotin-BSA) were purchased from Abcam Co., Ltd. (Shanghai, China). Gold nanoparticle-based biosensor materials, including sample pad, conjugate pad, nitrocellulose (NC) membrane, and absorbent pad were manufactured and assembled by HuiDeXing Biotech. Co., Ltd. (Tianjing, China) according to the design instructions of this study (Figure 2 ).
5
Rapid LAMP-Based HCV Diagnosis
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Crimson dye streptavidin-coated AuNPs (40 ± 5 nm, 10 mg/ml) were obtained from Bangs Laboratories Inc. (Fishers, IN, USA). Nitrocellulose (NC) membranes were purchased from Merck Millipore Co., Ltd. (Darmstadt, Germany). Rabbit anti-fluorescein antibody (anti-FAM; 0.2 mg/ml) and biotinylated bovine serum albumin (biotin-BSA; 4 mg/ml) were obtained from Abcam Co., Ltd. (Shanghai, China). The AuNPs-LFB containing sample pad, conjugate pad, NC membranes, and absorption pad were laminated on a plastic adhesive backing by HuiDeXin Biotech. Co., Ltd. (Tianjin, China) according to our design scheme (Fig. 1 A). LAMP reaction kits containing colorimetric indicator (leuco-hydroxynaphthol blue, L-HNB) were purchased from HaiGene Biotech Co., Ltd. (Harbin, China), and stored at -20 ℃. Nucleic acid releasing agents were purchased from GenDx Biotech Co., Ltd. (Suzhou, China) (stored at -20 ℃). Commercial RT-qPCR diagnostic kits for HCV were purchased from Xi’an Tianlong Technology Co., Ltd. (Xi’an, China) (stored at -20 ℃).
6
Isothermal Amplification-based Lateral Flow Biosensor
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The kit Qiagen QIAamp DNA mini kit (Qiagen, Beijing, China) was used to extract genomic DNA from fungi, bacterial and clinical. Isothermal amplification kits and colorimetric indicator (malachite green, MG) were provided by Beijing-Hai Tai Zheng Yuan Technology Co., Ltd. (Beijing, China). Dye streptavidin-coated polymer nanoparticles (Crimson red) were obtained from Bangs Laboratories, Inc. (Indiana, United States). Anti-FAM (rabbit anti-fluorescein antibody) and biotin-BSA (biotinylated bovine serum albumin) were obtained from Abcam. Co., Ltd. (Shanghai, China). Polymer nanoparticle-based lateral flow biosensor (LFB) materials, including sample pad, nitrocellulose membrane (NC), absorbent pad, conjugate pad and backing card were obtained from Jie-Yi Biotechnology. Co., Ltd. (Shanghai, China). Primers and labeled primers (Table 2 and Supplementary Table 1 ) used were synthesized by Beijing-Tsingke Biotechnology Co., Ltd. (Beijing, China).
7
Universal Isothermal Amplification and CRISPR-Cas12b Detection
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The universal isothermal amplification kits, colorimetric indicator (malachite green), gold nanoparticle–based LFB, and CRISPR-Cas12b protein (C2c1) were obtained from HuiDeXin Biotechnology (Tianjin, China). Anti-FAM (rabbit anti-fluorescein antibody) and biotin-BSA (biotinylated bovine serum albumin) were obtained from Abcam Co., Ltd. (Shanghai, China). The LFB materials, including a sample pad, an absorbent pad, a conjugate pad, a nitrocellulose membrane (NC), and a backing card, were purchased from the Jie-Yi Biotechnology. Co., Ltd. (Shanghai, China). A dye (crimson red) and streptavidin-coated gold nanoparticles (size, 34.46 ± 4.34 nm; extinction coefficient, 6.0 × 109 M-1 cm-1 at 506 nm) were purchased from Bangs Laboratories, Inc. (Indiana, United States). A real-time turbidimeter (LA-500) was purchased from Eiken Chemical Co., Ltd. (Japan).
8
Viral RNA Extraction and Isothermal Amplification
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Viral RNA extraction kits (QIAamp Viral RNA minikits; Qiagen, Hilden, Germany) (Cat NO. 52906) were purchased from Qiagen (Beijing, China). Universal isothermal amplification kits, AMV Reverse Transcriptase, colorimetric indicator (malachite green, MG), and biotin-14-dCTP were obtained from Bei-Jing HaiTaiZhengYuan. Co., Ltd. (Beijing, China). The LFB materials, including the backing card, sample pad, absorbent pad, conjugate pad, and nitrocellulose membrane (NC), were purchased from Jie-Yi Biotechnology. Co., Ltd. (Shanghai, China). Anti-FAM (rabbit anti-fluorescein antibody) and biotin-BSA (biotinylated bovine serum albumin) were purchased from Abcam. Co., Ltd. (Shanghai, China). Dye (Crimson red) streptavidin-coated polymer nanoparticles (129 nm, 10 mg ml-1; 100 mM borate, pH 8.5, with 0.1% BSA, 0.05% Tween 20 and 10 mM EDTA) were purchased from Bangs Laboratories, Inc. (Indiana, USA).
9
Rapid Molecular Detection of Neisseria gonorrhoeae
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Thayer-Martin (TM) chocolate agar plates were obtained from Autobio Biotechnology Co., Ltd. (Zhengzhou, China). Nucleic acid–releasing agents were obtained from Sansure Biotech Inc. (Changsha, China). Universal isothermal amplification kits and a colorimetric indicator (malachite green, MG) were purchased from HuiDeXin Bio-technology (Tianjin, China). Anti-FAM (rabbit anti-fluorescein antibody) and biotin BSA (biotinylated bovine serum albumin) were purchased from Abcam Co., Ltd. (Shanghai, China). Streptavidin dye–coated polymer nanoparticles (Crimson red) were obtained from Bangs Laboratories, Inc. (Indiana, United States). Polymer nanoparticle–based lateral flow biosensor (LFB) materials, including the sample pad, conjugate pad, absorbent pad, nitrocellulose (NC) membrane, and backing card, were obtained from HuiDeXin Bio-technology (Tianjin, China). N. gonorrhoeae PCR diagnosis kits were purchased from DaAn Gene Co., Ltd. (Guangzhou, China).
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