Mouse monoclonal anti β actin antibody

Mouse polyclonal anti-Cap antibody (Abcam, United Kingdom, 1:500), rabbit polyclonal anti-LC3B antibody (Sigma, United States), 1:1000), mouse monoclonal anti-β-actin antibody (NOVUS, United States), 1:1000), rabbit anti-PERK polyclonal antibody (Abcam, United Kingdom, 1:1000), rabbit anti-PERK antibody (phospho T982) polyclonal antibody (Abcam, United Kingdom, 1:1000), rabbit anti-p-eIF2α monoclonal antibody (NOVUS, United States), 1:1000), rabbit anti-eIF2α polyclonal antibody (Enzo Life, United States), 1:1000), rabbit anti-ATF4 polyclonal antibody (Proteintech, United States), 1:1000), rabbit monoclonal antibodies to anti-p-mTOR (Abcam, United Kingdom, 1:1000), and anti-p-AMPK (Abcam, United Kingdom, 1:1000), as well as rabbit polyclonal antibodies to anti-mTOR (Abcam, United Kingdom, 1:1000), anti-p-ERK1/2 and anti-ERK1/2 (CST, United States), 1:1000), and anti-AMPK (CST, United States), 1:1000). HRP (Horseradish peroxidase) Conjugated Goat Anti-Rabbit IgG (H+L) (Abbkine, United States), 1:5000) or Goat anti-Mouse IgG (H+L)-HRP (SUNGENE, China, 1:2000).

Fetal bovine serum (FBS) was purchased from HyClone (Waltham, MA, USA). Reverse transcriptase and SYBR green reagent were obtained from ThermoFisher (ThermoFisher Scientific, Waltham, MA, USA). Antibodies were purchased from different companies: rabbit monoclonal anti-BDNF (abcam, Cambridge, MA, USA), rabbit polyclonal anti-PDE4B (abcam), mouse monoclonal anti-CREB5 (ThermoFisher Scientific), rabbit monoclonal anti-phospho-CREB antibody (Cell signaling, Danvers, MA, USA) and mouse monoclonal anti-β-actin antibody (Novus, Centennial, CO, USA). Unless otherwise specified, all the other chemicals and reagents used in this study were from Sigma Chemicals (St. Louis, MO, USA).