Mice were sacrificed and BALF samples were collected by slow infusion and extraction with 0.2 ml cold PBS 4 times. The procedure was repeated four times and 0.8 ml BALF per mouse was collected for subsequent research. The concentration of total proteins in BALF was estimated using a BCA protein assay kit (cat. no. P0010; Beyotime Institute of Biotechnology). The levels of SOD and MDA in BALF were measured using a commercial SOD assay kit (cat. no. A001-3-2; Nanjing Jiancheng Bioengineering Institute) and MDA activity kit (cat. no. A003-4-1; Naning Jiancheng Bioengineering Institute), according to the manufacturer's instructions.
Mda activity kit
Manufactured by Nanjing Jiancheng
Sourced in China
The MDA activity kit is a laboratory equipment product that measures the activity of malondialdehyde (MDA), a biomarker for oxidative stress. The kit provides the necessary reagents and protocols to quantify MDA levels in biological samples. The core function of this product is to enable researchers to assess oxidative stress conditions in their studies.
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Lab products found in correlation
2 protocols using mda activity kit
1
Bronchoalveolar Lavage Fluid Analysis
2
Oxidative Stress Quantification in H9C2 Cells
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The production of ROS was detected by using Dichlorofluorescein diacetate (DCFH-DA) assay. Briefly, cells were incubated with 10 μmol/L DCFH-DA at 37°C in the dark for 20 min. Then, the cells were washed twice with PBS, and the fluorescence intensity was detected by using a fluorescence spectrophotometer (BioTek).
H9C2 cells were seeded in 96-well plates at a density of 1 × 104 cells/well. Myocardial tissues were homogenized and the supernatant was obtained. The activity of MDA was measured by using MDA activity kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China).
H9C2 cells were seeded in 96-well plates at a density of 1 × 104 cells/well. Myocardial tissues were homogenized and the supernatant was obtained. The activity of MDA was measured by using MDA activity kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China).
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