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Kdr pe

Manufactured by R&D Systems
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The KDR-PE is a laboratory equipment product designed for biological research. It is a precision instrument used for various applications in life science research. The core function of the KDR-PE is to perform accurate measurements and analyses in a controlled laboratory setting.

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Lab products found in correlation

Cd45 fitc, by BD (2 mentions) Cd11b apc cy7, by BioLegend (2 mentions) Cx3cr1 pe, by BioLegend (2 mentions) Cd235a apc, by BD (2 mentions) Lsr 2, by BD (2 mentions) Clone hip8, by BD (2 mentions) Fab357p, by R&D Systems (2 mentions) Clone 89106, by BD (2 mentions) Clone ga r2, by BioLegend (2 mentions) Cd41 apc cy7, by BioLegend (2 mentions) Cd43 percp cy5.5, by BD (2 mentions) Clone 1g10, by BD (2 mentions) Clone hi3o, by BioLegend (2 mentions) Cd8 pe, by Thermo Fisher Scientific (1 mentions) Cd34 pe, by BD (1 mentions) Cd34 apc, by BD (1 mentions) Cd4 alexa fluor 700, by Thermo Fisher Scientific (1 mentions) Cd45 apc efluor780, by Thermo Fisher Scientific (1 mentions) Cd5 pe cy7, by Thermo Fisher Scientific (1 mentions) Cd7 alexa fluor 700, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

VEGFR2/KDR-PE (3 citations) Anti-KDR-PE (3 citations) KDR-PE (FAB357P, (2 citations) Anti‐VEGF R2/KDR‐PE, clone 89,106 (2 citations) PE-conjugated anti-KDR (2 citations)

4 protocols using kdr pe

1

Multicolor Flow Cytometry Panel for Immune Cell Profiling

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The following antibodies were used for these studies: CD4-Alexa Fluor 700 (catalog #56-0048-82), CD5-PE-Cy7 (#25-0059-42), CD7-Alexa Fluor 700 (#56-0079-42), CD31-PerCP/eFluor710 (#46-0319-42), CD45-APC-eFluor780 (#47-0459-42), and CD144-PE (#12-1449-82) from eBioScience; CD8-PE (#555367), CD34-PE (#550761), and CD34-APC (#555824) from BD Pharmingen; KDR-PE (#359904) from BioLegend; and KDR-PE (#FAB357P100) from R&D Systems. Stained cells were analyzed using an LSRII (BD Biosciences) flow cytometer at the indicated time points. Data analysis was performed using FlowJo software. For T-lymphoid studies, analyses were carried out by gating on live cells, as indicated by lack of DAPI uptake, followed by gating on CD45+ cells. Gates were set using appropriate isotype controls.
Li Y., Brauer P.M., Singh J., Xhiku S., Yoganathan K., Zúñiga-Pflücker J.C, & Anderson M.K. (2017). Targeted Disruption of TCF12 Reveals HEB as Essential in Human Mesodermal Specification and Hematopoiesis. Stem Cell Reports, 9(3), 779-795.
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2

Multiparameter Flow Cytometry Profiling

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Cells were dissociated with Accutase for 20min and resuspended in FACS buffer containing 1% BSA, 2mM EDTA, 30ug/mL DNAse I and Normocin in PBS. Cells were washed and incubated in FACS buffer with antibody for 30 minutes on ice at 4 degrees in the dark. Cells were washed and resuspended in FACS buffer and strained through 40uM caps to eliminate cell clumps. Data collection was done on a BD-LSRII and FACSDiva v8. Gating (Fig. S2) and subsequent analysis was done using FlowJo software v10.5.3. FACS antibodies used include KDR-PE – R&D, FAB357P, Clone 89106 at 1:60, CD235A-APC – BD Biosciences at 1:100, 551336, Clone GA-R2, CD41-Apc/Cy7 – Biolegend, 303715, Clone HIP8, CD43-PerCP/Cy5.5 -BD Biosciences, 563521, Clone 1G10, CD45-FITC – BD Biosciences, 560976, Clone HI3O, CX3CR1-PE – Biolegend, 341604, 2A9–1, and Cd11b-APC/Cy7 – Biolegend, 301351, ICRF44 all at 5ul/100ul test.
Guttikonda S.R., Sikkema L., Tchieu J., Saurat N., Walsh R., Harschnitz O., Ciceri G., Sneeboer M., Mazutis L., Setty M., Zumbo P., Betel D., de Witte L.D., Pe’er D, & Studer L. (2021). Fully defined human pluripotent stem cell-derived microglia and tri-culture system model C3 production in Alzheimer’s disease. Nature neuroscience, 24(3), 343-354.
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3

Quantification of Endothelial and Microparticles

Cited 1 time
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Blood samples were obtained after 12 hours of fasting and the analyses were
performed at the central laboratory of our university. All athletes were
allowed to maintain their daily exercises program even on the day before
blood sample collection. The athletes had very similar exercise training
programs, corresponding to two long-distance running sessions every day, 15
km in the morning and 10 km in the afternoon, and intensive training
(100-1,000 meter shots, repeated many times)twice a week, on Tuesday and
Thursday mornings. All blood samples were collected on Thursdays, before
exercise.
Measurements of EPCs and MPs were performed as previously reported, using
fresh blood samples in EDTA containing tubes.12 (link)-15 (link)For determination of EPCs, a minimum of 500,000 events was acquired by
flow-cytometry (FACSCalibur, BD Biosciences, USA). Fluorescently labeled
mouse anti-human antibodies were used for EPCs (CD34 FITC, BD Biosciences,
USA; CD133 APC, Miltenyi Biotec, USA; KDR PE, R&D Systems, USA), PMPs
(CD42 FITC and CD31 PE, BD Biosciences, USA) and EMPs (CD51 FITC, BD
Biosciences). Disposable containers (BD Biosciences) were used to quantify
the number of microparticles per microliter of
platelet-poor plasma (PPP).
Bittencourt C.R., Izar M.C., França C.N., Schwerz V.L., Póvoa R.M, & Fonseca F.A. (2017). Effects of Chronic Exercise on Endothelial Progenitor Cells and Microparticles in Professional Runners. Arquivos Brasileiros de Cardiologia, 108(3), 212-216.
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4

Multiparameter Flow Cytometry Profiling

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Cells were dissociated with Accutase for 20min and resuspended in FACS buffer containing 1% BSA, 2mM EDTA, 30ug/mL DNAse I and Normocin in PBS. Cells were washed and incubated in FACS buffer with antibody for 30 minutes on ice at 4 degrees in the dark. Cells were washed and resuspended in FACS buffer and strained through 40uM caps to eliminate cell clumps. Data collection was done on a BD-LSRII and FACSDiva v8. Gating (Fig. S2) and subsequent analysis was done using FlowJo software v10.5.3. FACS antibodies used include KDR-PE – R&D, FAB357P, Clone 89106 at 1:60, CD235A-APC – BD Biosciences at 1:100, 551336, Clone GA-R2, CD41-Apc/Cy7 – Biolegend, 303715, Clone HIP8, CD43-PerCP/Cy5.5 -BD Biosciences, 563521, Clone 1G10, CD45-FITC – BD Biosciences, 560976, Clone HI3O, CX3CR1-PE – Biolegend, 341604, 2A9–1, and Cd11b-APC/Cy7 – Biolegend, 301351, ICRF44 all at 5ul/100ul test.
Guttikonda S.R., Sikkema L., Tchieu J., Saurat N., Walsh R., Harschnitz O., Ciceri G., Sneeboer M., Mazutis L., Setty M., Zumbo P., Betel D., de Witte L.D., Pe’er D, & Studer L. (2021). Fully defined human pluripotent stem cell-derived microglia and tri-culture system model C3 production in Alzheimer’s disease. Nature neuroscience, 24(3), 343-354.
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