Mms 466s

Manufactured by BioLegend

The MMS-466S is a laboratory equipment product. It functions as a Microplate Magnetic Separator.

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Lab products found in correlation

Alexa fluor 594, by Thermo Fisher Scientific (2 mentions) Hoechst 33342 solution, by Thermo Fisher Scientific (2 mentions) Strataquest analysis system, by TissueGnostics (2 mentions) Alexa fluor 488, by Jackson ImmunoResearch (2 mentions) Tissuefaxs plus, by TissueGnostics (2 mentions) Anti s100a9, by Proteintech (1 mentions) Anti α sma, by BioLegend (1 mentions)

2 protocols using mms 466s

Immunofluorescent Analysis of Human Pancreas Tissue

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Wax-embedded human pancreas blocks were cut into 5-μm-thick sections and dewaxed, and antigens were retrieved by Trilogy™ (Cell Marque) treatment at 121 °C for 3 min. Sections were blocked with 3% BSA for 30 min and then incubated overnight with appropriate primary antibodies (anti-S100A9, 1:200, 26,992–1-AP, Proteintech; anti-α-SMA, 1:200, MMS-466S, BioLegend). After washing in DPBS, the pancreas sections were further incubated with fluorophore-conjugated secondary antibodies (Alexa Fluor 488, 1:300, Jackson ImmunoResearch. Alexa Fluor 594, 1:300, A-21207, Thermo Fisher Scientific) for 1 h. Hoechst 33,342 Solution (1:10,000, Thermo Fisher Scientific) was used for staining nuclei. Images were captured by TissueFAXS PLUS (TissueGnostics, Vienna, Austria) and quantified by the StrataQuest Analysis System (TissueGnostics).

Kung P.J., Lai T.Y., Cao J., Hsu L.C., Chiang T.C., Ou-Yang P., Tsai C.Y., Tsai Y.F., Lin C.W., Chen C.C., Tsai M.K., Tien Y.W, & Lee C.Y. (2021). The role of S100A9 in the interaction between pancreatic ductal adenocarcinoma cells and stromal cells. Cancer Immunology, Immunotherapy, 71(3), 705-718.

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Immunofluorescent Analysis of Pancreatic Tissue

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Wax-embedded human pancreas blocks were cut into 5 µm-thick sections and dewaxed, and antigens were retrieved by TrilogyTM (Cell Marque) treatment at 121 °C for 3 minutes. Sections were blocked with 3% BSA for 30 minutes and then incubated overnight with appropriate primary antibodies (anti-S100A9, 1:200, 26992-1-AP, Proteintech; anti-α-SMA, 1:200, MMS-466S, BioLegend). After washing in DPBS, the pancreas sections were further incubated with fluorophore-conjugated secondary antibodies (Alexa Fluor 488, 1:300, Jackson ImmunoResearch. Alexa Fluor 594, 1:300, A-21207, Thermo Fisher Scientific) for 1 hour. Hoechst 33342 Solution (1:10,000, Thermo Fisher Scientific) was used for staining nuclei. Images were captured by TissueFAXS PLUS (TissueGnostics, Vienna, Austria) and quantified by the StrataQuest Analysis System (TissueGnostics).

Kung P., Lai T., Cao J., Hsu L., Chiang T., Ou‐Yang P., Tsai C., Tsai Y., Lin C., Cc C., Tsai M., Tien Y., & Lee C. (2020). The Role of S100A9 in the Interaction Between Pancreatic Ductal Adenocarcinoma Cells and Stromal Cells.

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