Log In Sign up
The largest database of trusted experimental protocols

Primescript rt2 first strand kit

Manufactured by Qiagen
Visit Supplier

The PrimeScript RT2 First Strand Kit is a laboratory equipment product that enables the synthesis of high-quality cDNA from RNA samples. It includes the necessary reagents and enzymes to efficiently reverse transcribe RNA into first-strand cDNA.

Automatically generated - may contain errors

Lab products found in correlation

Rneasy mini kit, by Qiagen (2 mentions) Gapdh, by Qiagen (1 mentions)

Spelling variants of this product

RT2 First Strand Kit (1647 citations)

2 protocols using primescript rt2 first strand kit

1

ER Stress Response in HBV-Infected Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
LX-2 cells were incubated with conditioned media from HepG2, HepG2.2.15, or PXB infected with or without HBV for 24 hours. Cellular RNA was extracted using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions. One microgram of RNA was reverse-transcribed with a PrimeScript RT2 First Strand Kit (Qiagen). A human ER stress-associated signaling target PCR array was performed according to the manufacturer's protocol [14 (link)]. The data were analyzed using PCR Array Data Analysis Software (http://www.sabiosciences.com/pcrarraydataanalysis.php).
Sasaki R., Kanda T., Nakamura M., Nakamoto S., Haga Y., Wu S., Shirasawa H, & Yokosuka O. (2016). Possible Involvement of Hepatitis B Virus Infection of Hepatocytes in the Attenuation of Apoptosis in Hepatic Stellate Cells. PLoS ONE, 11(1), e0146314.
+ Open protocol
+ Expand
2

Analyzing NF-κB Signaling Targets

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cellular RNA was extracted using the RNeasy Mini Kit (Qiagen). One microgram of RNA was reverse-transcribed with a PrimeScript RT2 First Strand Kit (Qiagen). PCR was performed on cDNA templates using primers specific for B-cell CLL/lymphoma (BCL2), BCL2-like 1 (BCL2L1/BCL-X/BCLXL) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH), which were purchased from Qiagen. A Human NF-κB Signaling Targets Real-time RT-PCR Array was performed according to the manufacturer's protocol. The data were analyzed by PCR Array Data Analysis Software (http://www.sabiosciences.com/pcrarraydataanalysis.php).
Jiang X., Kanda T., Wu S., Nakamoto S., Nakamura M., Sasaki R., Haga Y., Wakita T., Shirasawa H, & Yokosuka O. (2015). Hepatitis C Virus Nonstructural Protein 5A Inhibits MG132-Induced Apoptosis of Hepatocytes in Line with NF-κB-Nuclear Translocation. PLoS ONE, 10(7), e0131973.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!