Wide bore 89 mm 9.4 t vertical bore magnet

To determine the structural and functional abnormalities of hearts in mice affected with myocarditis, we used MRM imaging as a non-invasive tool [32 (link)]. Briefly, mice were anaesthetized using 2% isoflurane; a rectal thermometer was placed in the rectum to monitor body temperature, and a pulse oximeter was connected to monitor the heart rate. An animal monitoring system involving respirometry and pulse oximetry was used to minimize image blurring due to respiratory and cardiac movements. Animals were subjected to MRM imaging using a wide-bore (89 mm) 9.4 T vertical-bore magnet (Varian, Inc. Walnut Creek, CA) equipped with triple axis gradients of 100 G/cm and a 4 cm radio-frequency imaging coil as described previously [32 (link)]. Using an echo-based cine pulse sequence, short-axis slices of hearts were captured in eight time frames and stored. The images were analyzed using Segment software (Segment v1.8 R1430, Medviso, Sweden) to determine the changes, if any, in the structural (left ventricular [LV] wall thickness) and functional parameters (end diastolic volume and ejection fraction).

MRM imaging was used to determine cardiac abnormalities in mice immunized with SERCA2a 971-990 during days, 38 to 39 as we have described previously (50 (link), 58 ). Anesthetized animals were placed in the animal holder equipped with respirometry and pulse oximetry to gate the respiratory and cardiac signals, and body temperature was monitored using a rectal thermometer. MRM imaging was performed using a wide-bore (89 mm) 9.4 T vertical-bore magnet (Varian, Inc., Walnut Creek, CA) equipped with triple axis gradients of 100 G/cm and a 4-cm radio-frequency imaging coil. Short axis slices (images) of hearts captured in eight-time frames using an echo-based cine pulse sequence were analyzed using Segment software (Segment v1.8 R1430, Medviso, Sweden) to assess LV wall thickness, EDV, ESV, stroke volume (SV) and ejection fraction (EF).