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Cd45 apc h7

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CD45-APC-H7 is a fluorophore-conjugated antibody that binds to the CD45 antigen, which is expressed on the surface of leukocytes. It is a commonly used marker for the identification and analysis of various immune cell populations.

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Lab products found in correlation

Cd90 pe, by BD (1 mentions) Cd105 percp cy5, by BD (1 mentions) Cd31 pe cy7, by BD (1 mentions) Cd44 apc h7, by BD (1 mentions) Cd73 bv421, by BD (1 mentions) Cd140a bv421, by BD (1 mentions) Cd140b percp cy5, by BD (1 mentions) Cd271 apc, by Miltenyi Biotec (1 mentions) Cd146 fitc, by BD (1 mentions) Facsverse system, by BD (1 mentions) Cd4 fitc, by BioLegend (1 mentions) Dnase 1, by AppliChem (1 mentions) Collagenase type p, by Merck Group (1 mentions) Mhcii pe, by BioLegend (1 mentions) Cd8 pe cy7, by BioLegend (1 mentions) Facscanto flow cytometer, by BD (1 mentions) Hepes, by Lonza (1 mentions) Cd3 pe, by BD (1 mentions) Lsrfortessa flow cytometer, by BD (1 mentions) Cd8 fitc, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Anti-CD45 APC-H7 CD45-APC

3 protocols using cd45 apc h7

1

Characterizing Synovial MSC Surface Markers

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Cultured synovial MSCs from three donors at passage 1 were harvested using a cell-dissociation buffer. Cells were suspended in HBSS at a density of 5 × 105 cells/mL and stained for 30 minutes on ice with the antibodies CD31-PE-Cy7 (Becton, Dickinson and Company; BD, Franklin Lakes, NJ, USA), CD45-APC-H7 (Biolegend, San Diego, CA, USA), CD44-APC-H7 (BD), CD73-BV421 (BD), CD90-PE (BD), CD105-PerCP-Cy5.5 (BD), CD140a-BV421 (BD), CD140b-PerCP-Cy5.5 (BD), CD146-FITC (BD) and CD271-APC (Miltenyi Biotec) for cell surface analysis. Flow cytometric analysis of the cell surface was performed by a triple-laser FACS Verse™ system (BD).
Mizuno M., Katano H., Otabe K., Komori K., Matsumoto Y., Fujii S., Ozeki N., Tsuji K., Koga H., Muneta T., Matsuyama A, & Sekiya I. (2015). Platelet-derived growth factor (PDGF)-AA/AB in human serum are potential indicators of the proliferative capacity of human synovial mesenchymal stem cells. Stem Cell Research & Therapy, 6, 243.
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2

Multiparameter Flow Cytometry of Lymph Nodes

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For flow cytometric analysis of LN cellularity, inguinal LNs from individual mice were pooled and digested on 37°C in RPMI containing 2% FCS, 20 mM Hepes (all from Lonza), 1 mg/ml Collagenase Type P (Sigma-Aldrich), and 25 μg/ml DNaseI (AppliChem) for 20 min. After enzymatic digestion, cell suspensions were washed with PBS and stained using the following antibodies: CD3-PE (BD Bioscience), CD8-PeCy7, CD4-FITC, CD45-APC-H7, MHCII-PE, CD11c-PeCy7, B220-APC (BioLegend). In flow cytometric analyses, 7-amino-actinomycin D (7AAD; Calbiochem) was used to discriminate dead cells. Samples were analyzed by flow cytometry using a FACSCanto flow cytometer (BD Biosciences); data were analyzed using FlowJo software (Tree Star).
Novkovic M., Onder L., Cupovic J., Abe J., Bomze D., Cremasco V., Scandella E., Stein J.V., Bocharov G., Turley S.J, & Ludewig B. (2016). Topological Small-World Organization of the Fibroblastic Reticular Cell Network Determines Lymph Node Functionality. PLoS Biology, 14(7), e1002515.
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3

Multiparametric Cell Characterization

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Characterization of cells isolated from human samples was performed by using surface staining with antibodies against CD326/epithelial cell adhesion molecule (EpCAM)-fluorescein isothiocyanate (FITC; Miltenyi Biotec), CD45-phycoerythrin (PE)-Cy7, CD31/platelet endothelial adhesion molecule 1-allophycocyanin (APC), podoplanin (PDPN)-PE, CD90/Thy-1-PE, CD54/intercellular adhesion molecule 1 (ICAM-1)-FITC, CD105/ endoglin-peridinin-chlorophyll-protein complex (PerCP)-Cy5 (all from BioLegend, San Diego, Calif), CD44-APC-Cy7, and CD73-APC (all from BD Biosciences, San Jose, Calif). Mouse cell characterization was performed with antibodies against PDPN-PE, CD31-APC, CD8-FITC, Ly5.1-APC, platelet-derived growth factor receptor a-biotin, ICAM-1-biotin (all from eBioscience, San Diego, Calif), CD45-APC-H7, anti-Ter-119/erythroid (TER119)-APC-H7, vascular cell adhesion molecule 1 (VCAM-1)-PerCP, anti-EpCAM-PE-Cy7 (all from BioLegend), and CD62 ligand (CD62L)-FITC (Miltenyi Biotec). Seven-aminoactinomycin D (Calbiochem, San Diego, Calif) was used to discriminate dead cells in flow cytometric analyses. Samples were analyzed by using flow cytometry with FACSCanto II and LSR Fortessa flow cytometers (BD Biosciences); data were analyzed with FlowJo software (TreeStar, Ashland, Ore).
Cheng H.W., Onder L., Cupovic J., Boesch M., Novkovic M., Pikor N., Tarantino I., Rodriguez R., Schneider T., Jochum W., Brutsche M, & Ludewig B. (2018). CCL19-producing fibroblastic stromal cells restrain lung carcinoma growth by promoting local antitumor T-cell responses. The Journal of allergy and clinical immunology, 142(4).
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