Rab35

Fibronectin, monastrol and MG132 were purchased from Sigma. Transferrin conjugated to AlexaFluor 568, Phalloidin-568, DAPI, as well as all secondary antibodies used for immunofluorescence were purchased from Molecular Probes. The following primary antibodies were used: EHD1(54 (link)), MICAL-L1 (Novus Biologicals), affinity-purified rabbit polyclonal peptide antibody directed against the C-terminus of EHD2 (VERGPDEAMEDGEEGSDDEA) (AnaSpec), α-tubulin (Molecular Probes), anti-human anticentromere (ACA; Antibodies Inc.), pericentrin, giantin, MKLP1 and PLK1 (Abcam), Pan-actin (Millipore), Rab11 (US Biologicals), Rab35 (Protein Tech), Aurora B (Abnova; generous gift from Dr. Jixin Dong) and EEA1 (Cell Signaling Technologies).

Antibodies to myc tag, Exportin-5 (western blotting), β-actin, were purchased from Cell Signaling Technologies. Mouse monoclonal antibody to Exportin-5 used for immunofluorescence was obtained from Abnova. Antibodies against phospho-serine, Argonaute-2, alpha smooth muscle actin, and Dicer were purchased from Abcam. Antibody to GRP1(CYTH3) was purchased from GeneTex. Alpha-tubulin, MT1-MMP, and Fascin antibodies were purchased from Millipore Sigma. Mouse monoclonal anti-HA tag antibody was obtained from Covance. CYTH2,RhoA, Ran, and GAPDH antibodies were purchased from Santa Cruz Biotechnology. VAMP3 and Anti-GFP antibodies were purchased from ThermoFisher Scientific. Rab35, MHC-I, Rab22a, CYTH1, RanGAP, SMAD-7 and ARF6 (immunofluorescence) antibodies were purchased from Proteintech. TIMP3 antibody was purchased from R&D Technolgies. Mouse Monoclonal antibody to ARF6 was described previously^{67 (link)}. Fluorophore conjugated secondary antibodies (donkey anti-mouse FITC, donkey anti-rabbit TRITC) were purchased from Jackson Immunoresearch. Antibody usage information is contained in Supplementary Table 2. SecinH3 and TBB were purchased from Cayman Chemical.