Ab118459

Manufactured by Abcam

Ab118459 is an antibody that targets Claudin 7. It is suitable for use in various laboratory applications such as Western Blot and Immunocytochemistry.

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Lab products found in correlation

2 protocols using ab118459

Antibody Panel for Platelet and Cytoskeleton Characterization

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Anti-human protein antibodies used for platelet and imMKCL cell IB and IF staining were as follows: rabbit polyclonals to ARPC1A (Sigma-Aldrich, HPA004334; dilutions 1/250 or 1/500 for IB, 1/25 for IF), ARPC1B (Sigma-Aldrich, HPA004832; dilutions 1/100 or 1/500 for IB, 1/100 for IF), ARPC2/p34ARC (Millipore, 07-227; dilution 1/1,000 for IB), ARPC3 (Sigma-Aldrich, HPA006550; dilution 1/1,000 for IB), ARPC5/p16ARC (Abcam, ab118459; dilutions 1/1,000 for IB, 1/100 for IF) and α-tubulin (Cell Signaling, 11H10; dilutions 1/1,000 for IB, 1/100 for IF); rabbit monoclonals to ARP2 (Abcam, ab128934; dilution 1/1,000 for IB), ARP3 (Abcam, ab151729; dilution 1/1,000 for IB) and WASP (Cell Signaling, D9C8; dilution 1/1,000 for IB); mouse monoclonals to γ-tubulin (Thermo Scientific, 4D11), α-tubulin (Sigma-Aldrich, Clone B-5-1-2; dilutions 1/1,000 for IB, 1/100 for IF), CD61 (Dako, Clone Y2/51; dilution 1/200 for IF), TSP1 (R&D Systems, clone 301221; dilution 1/100 for IF) and GAPDH (Millipore, MAB374; dilution 1/5,000 for IB), and a goat polyclonal to P-selectin (Santa Cruz, sc-6941; dilution 1/100 for IF).

Kahr W.H., Pluthero F.G., Elkadri A., Warner N., Drobac M., Chen C.H., Lo R.W., Li L., Li R., Li Q., Thoeni C., Pan J., Leung G., Lara-Corrales I., Murchie R., Cutz E., Laxer R.M., Upton J., Roifman C.M., Yeung R.S., Brumell J.H, & Muise A.M. (2017). Loss of the Arp2/3 complex component ARPC1B causes platelet abnormalities and predisposes to inflammatory disease. Nature Communications, 8, 14816.

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Molecular Signaling in Aortic Aging

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Proteins from the human aortic wall tissue of patients, aortic wall of mice, and cultured MOVAs and MAECs were extracted with RIPA lysis buffer (Thermo Fisher Scientific Inc.) supplemented with a protease and phosphatase inhibitor cocktail. Western blotting was performed using antibodies against NAMPT (Abcam, ab236874), P16 (Abcam, ab118459), P21 (Abcam, Ab109520), eNOS (Abcam, ab199956), P-eNOS (Abcam, ab32419), SIRT1 (Abcam, ab110304), MMP-2 (Abcam, ab97779), MMP-9 (Abcam, ab38898), AKT (CST, 4685S), P-AKT (CST, 4060S), P38 (CST, 8690S), P-P38 (CST, 4511S), ERK1/2 (Abcam, ab17942), P-ERK1/2 (Abcam, ab214362), JNK (Abcam, Ab179461), P-JNK (Abcam, Ab124956), and GAPDH (Abcam, ab8245), followed by an incubation with horseradish peroxidase-conjugated secondary antibodies (Santa Cruz Biotechnology) for 1 hour at room temperature. A gel imaging system (Tanon) and AlphaView software were used to image and analyze the Western blots.

Zhou L., Zhang S., Bolor-Erdene E., Wang L., Tian D, & Mei Y. (2020). NAMPT/SIRT1 Attenuate Ang II-Induced Vascular Remodeling and Vulnerability to Hypertension by Inhibiting the ROS/MAPK Pathway. Oxidative Medicine and Cellular Longevity, 2020, 1974265.

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