Adaptor tagged ds-cDNA libraries were constructed from total DNA free-RNA (1 µg per sample) using the TruSeq Sample Prep Kit V1 and V2 (Illumina, San Diego, CA). Each sample consisted of 10 pooled leafhoppers of the same cohort and transmission group. The quality of the cDNA libraries was assessed using the Experion Automated Electrophoresis System and quantified using the Qubit 2.0 Fluorometer (Life Technologies, Carlsbad, CA). Samples were diluted to 18 nM and pooled to generate the multiplexed cDNA library. In total, 12 adaptor-tagged samples were pooled, consisting of a healthy control and three treatments (transmitters, acquirers and non-acquirers) for each of the three replicate experiments.
Truseq sample prep kit v1 and v2
Manufactured by Illumina
The TruSeq Sample Prep Kit is a library preparation kit designed for Illumina sequencing platforms. It is available in two versions, V1 and V2, which provide the necessary reagents and protocols to convert DNA or RNA samples into sequencing-ready libraries. The kit includes components for sample fragmentation, end-repair, adapter ligation, and library amplification. It is compatible with a wide range of input material, including genomic DNA, FFPE samples, and total RNA.
Automatically generated - may contain errors
3 protocols using truseq sample prep kit v1 and v2
1
Adaptor Tagged ds-cDNA Sequencing Library
2
Aphid Total RNA Extraction and RNA-Seq Library Prep
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total RNA was extracted from the pools of 20 aphids using the RNeasy Mini Kit (QIAGEN, Germantown, MD). RNA quantity and quality was assessed using the Experion Automated Electrophoresis System (Bio-Rad, Hercules, CA). All samples selected for cDNA synthesis had RNA Quality Indicator values of 10.
RNA (1 µg/sample) was used to generate adaptor-ligated double-stranded cDNA libraries for RNA-Seq using the TruSeq Sample Prep Kit V1 and V2 (Illumina, San Diego, CA) following the manufacturer’s protocol. Quantification of ds-cDNA was done using the Qubit 2.0 Fluorometer (Life Technologies, Carlsbad, CA) and quality assessed using the Experion Automated Electrophoresis System (Bio-Rad, Hercules, CA). Samples were diluted to 17.5 nM and pooled to generate the multiplexed cDNA library (24 adaptor-tagged pooled samples total: 3 treatments × 2 time points × 4 replicates).
RNA (1 µg/sample) was used to generate adaptor-ligated double-stranded cDNA libraries for RNA-Seq using the TruSeq Sample Prep Kit V1 and V2 (Illumina, San Diego, CA) following the manufacturer’s protocol. Quantification of ds-cDNA was done using the Qubit 2.0 Fluorometer (Life Technologies, Carlsbad, CA) and quality assessed using the Experion Automated Electrophoresis System (Bio-Rad, Hercules, CA). Samples were diluted to 17.5 nM and pooled to generate the multiplexed cDNA library (24 adaptor-tagged pooled samples total: 3 treatments × 2 time points × 4 replicates).
3
RNA-Seq Library Preparation from Leafhoppers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total RNA was extracted from pools of 25 leafhoppers using the RNeasy Mini Kit (QIAGEN, Germantown, MD). RNA quantity and quality were assessed using the Experion Automated Electrophoresis System (Bio-Rad, Hercules, CA). Only samples with RNA Quality Indicator values of 10 were selected for cDNA synthesis.
RNA (800 ng per sample) was used to generate adaptor-ligated double-stranded cDNA libraries for RNA-Seq using the TruSeq Sample Prep Kit V1 and V2 (Illumina, San Diego, CA) following the manufacturer’s protocol. Quantification and quality inspection of ds-cDNA was carried out using the Experion Automated Electrophoresis System (Bio-Rad, Hercules, CA) and Qubit® 2.0 Fluorometer (Life Technologies, Carlsbad, CA). Samples were diluted to 20 nM and pooled to generate the multiplexed cDNA library (24 adaptor-tagged sample pools total: 3 treatments x 2 time points x 4 replicates).
RNA (800 ng per sample) was used to generate adaptor-ligated double-stranded cDNA libraries for RNA-Seq using the TruSeq Sample Prep Kit V1 and V2 (Illumina, San Diego, CA) following the manufacturer’s protocol. Quantification and quality inspection of ds-cDNA was carried out using the Experion Automated Electrophoresis System (Bio-Rad, Hercules, CA) and Qubit® 2.0 Fluorometer (Life Technologies, Carlsbad, CA). Samples were diluted to 20 nM and pooled to generate the multiplexed cDNA library (24 adaptor-tagged sample pools total: 3 treatments x 2 time points x 4 replicates).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!