After various treatments, the cells were fixed in 4% paraformaldehyde and then blocked with 5% goat serum. The cells were immunostained with anti-Smad1 antibody (Epitomics, Burlingame, CA, USA) or anti-OCN antibody (Cell Signaling) followed by a goat anti-rabbit Alexa Fluor-555- or Alexa Fluor-488-conjugated secondary antibody (Invitrogen). The cells were covered with an Anti-Fade Reagent (Cell Signaling).
Anti ocn antibody
Manufactured by Cell Signaling Technology
Sourced in United States
The Anti-OCN antibody is a laboratory tool used to detect and quantify the presence of osteocalcin, a protein involved in bone formation and mineralization. This antibody can be utilized in various techniques, such as Western blotting, immunohistochemistry, and ELISA, to study the expression and distribution of osteocalcin in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Antifade reagent, by Cell Signaling Technology (1 mentions)
Anti runx2 antibody, by Cell Signaling Technology (1 mentions)
Anti alp antibody, by Cell Signaling Technology (1 mentions)
Anti skp2 antibody, by Santa Cruz Biotechnology (1 mentions)
Anti foxo1 antibody, by Cell Signaling Technology (1 mentions)
Anti gapdh antibody, by Santa Cruz Biotechnology (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Ripa lysis buffer, by Beyotime (1 mentions)
2 protocols using anti ocn antibody
1
Immunocytochemical Analysis of Smad1 and OCN
2
Western Blot Analysis of Osteogenic Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Total protein was extracted from tissues or cells using RIPA Lysis Buffer (Beyotime, Beijing, China). The protein was then separated by 12% SDS-PAGE and transferred onto PVDF membranes (Millipore, USA). The membranes were blocked with 5% skim milk and incubated with primary antibodies, including anti-FOXO1 antibody (1:400, Cell Signaling Technology), anti-ALP antibody, anti-Runx2 antibody (1:300, Cell Signaling Technology), anti-Ostx antibody (1:300, Cell Signaling Technology), anti-OCN antibody (1:300, Cell Signaling Technology), anti-Skp2 antibody (1:500, Santa Cruz, USA) and anti-GAPDH antibody (1:800, Santa Cruz). Then, the membranes were incubated with anti-rabbit horseradish peroxidase-conjugate secondary antibody (1:2000, Santa Cruz) at room temperature for 1 h. ECL development solution (Thermo, USA) was used to visualize the proteins.
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