Liver TG were extracted by homogenization in a 5% NP40 solution followed by heating in an 80°C water bath. Samples were then centrifuged and the supernatant containing TG was saved. Liver cholesterol was extracted by homogenization in a 7:11:0.1 mix of chloroform:isopropanol:NP40. The lower organic phase was taken and air dried in the hood, and then redissolved according to the Cholesterol Quantification kit (Sigma Alderich, MO) instructions. Plasma and liver TG and cholesterol were determined using Triglyceride Colorimetric Assay kit (#MAK266, Sigma Aldrich, MO) and Cholesterol Quantification kit (MAK043, Sigma Aldrich, MO) according to manufacturer’s instruction.
Triglyceride colorimetric assay kit
Manufactured by Merck Group
Sourced in Macao
The Triglyceride Colorimetric Assay kit is a laboratory equipment product designed to measure the concentration of triglycerides in a sample. The kit utilizes a colorimetric method to quantify the triglyceride levels.
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Lab products found in correlation
2 protocols using triglyceride colorimetric assay kit
1
Liver Lipid Extraction and Quantification
2
Comprehensive Metabolic Panel Protocol
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Blood samples were obtained after an overnight fast. The level of blood glucose and plasma insulin was measured by glucometer (Roche, USA) and mouse insulin enzyme linked immunosorbent assay (ELISA) kit (90080, Crystal Chem, USA), respectively. Serum triglyceride, cholesterol, ALT, AST, BUN, and Cre levels were determined with an automatic biochemical analyzer (7170, Hitachi, Tokyo, Japan). The level of plasma NEFA was measured with a NEFA C Test Kit (#294‐63601, Wako Chemicals, Osaka, Japan). To test the liver triglyceride level, liver tissue was homogenized in a standard mix including chloroform/methanol (2:1). Subsequently, the organic phase in resolutions was dried down and redissolved in isopropanol. Liver triglyceride content was assessed using a Triglyceride Colorimetric Assay Kit (#MAK266‐1KT, Sigma‐Aldrich).
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