PCL (Mw = 80,000), PEP essential oil (purified by triple-distillation), standard menthol, and fetal bovine serum (FBS) were purchased from Sigma-Aldrich (Darmstadt, Germany). Glacial acetic acid (GAA, VWR, Darmstadt, Germany) was used as a solvent. Phosphate-buffered saline (PBS, biotech grade, pH 7.4) and dichloromethane (DCM) were obtained from VWR (Darmstadt, Germany). The microorganism strains of S. aureus (ATCC25923), and E. coli (ATCC25922) were used in our laboratory. Luria-Bertani (LB) agar and lysogeny broth (LB) medium were supplied by Carl Roth GmbH (Karlsruhe, Germany). Dulbecco's modified Eagle‘s medium (DMEM), penicillin/streptomycin (PS), and trypsin/EDTA were purchased from Thermo Scientific (Schwerte, Germany). NHDF cell line was obtained from Translation Research Center (TRC), Erlangen. All reagents and solvents were of analytical grade.
Pcl mw 80 000
Manufactured by Merck Group
Sourced in United States
PCL (Mw 80,000) is a synthetic polymer material used in various laboratory applications. It has a molecular weight of approximately 80,000 g/mol. The core function of this product is to serve as a raw material for further processing and development in research and scientific settings.
Automatically generated - may contain errors
Lab products found in correlation
Lysogeny broth medium, by Carl Roth (1 mentions)
Luria bertani agar, by Carl Roth (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Dichloromethane dcm, by Avantor (1 mentions)
Penicillin streptomycin p s, by Thermo Fisher Scientific (1 mentions)
Collagen type 4, by Merck Group (1 mentions)
N n dimethylformamide (dmf), by Merck Group (1 mentions)
1 ethyl 3 3 dimethylaminopropyl carbodiimide, by Merck Group (1 mentions)
N dimethylformamide, by Merck Group (1 mentions)
Collagen type 1, by Merck Group (1 mentions)
Chitosan, by Merck Group (1 mentions)
Chloroform, by Merck Group (1 mentions)
Acetic acid, by Merck Group (1 mentions)
Acetic acid, by Nacalai Tesque (1 mentions)
Hydroxyapatite tricalcium phosphate ceramic powder, by Zimmer Biomet (1 mentions)
Luria bertani medium, by Sangon (1 mentions)
Tio2 nanopowder, by Merck Group (1 mentions)
10 protocols using pcl mw 80 000
1
Antimicrobial Screening of PCL-Based Formulations
2
Fabrication of Collagen-based Biomaterials
3
Fabrication of Collagen-Chitosan Nanofiber Scaffolds
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PCL (Mw 80,000) (Sigma, New York, NY, USA) was dissolved in N-dimethylformamide and chloroform (Merck, Kenilworth, NJ, USA) by ratio 1/9 (N-dymethylformamid/chloroform). Spinning solution with concentration of 8% (w/v) was prepared. Then, the solution was electrospun upon applying a high voltage (22.5 kv) and mass flow rate of 1 ml/h at room temperature. Polymer nanofibers were collected on an aluminum foil which covered the target [1 (link)].
Collagen-chitosan film was developed by casting and solvent-evaporation method. Collagen (type I, Sigma) and chitosan (Sigma) were separately dissolved in acetic acid (0.5 M, Merck). Mixture of the 1% collagen and 1% chitosan solutions (9:1 V/V) were cast on polystyrene molds, frozen at -80℃ for 2 hours and then lyophilized in a freeze dryer for 24 hours. Scaffolds then cross-linked using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (Sigma). The sample was rinsed in distilled water and dried at 37℃ for 4 days.
Collagen-chitosan film was developed by casting and solvent-evaporation method. Collagen (type I, Sigma) and chitosan (Sigma) were separately dissolved in acetic acid (0.5 M, Merck). Mixture of the 1% collagen and 1% chitosan solutions (9:1 V/V) were cast on polystyrene molds, frozen at -80℃ for 2 hours and then lyophilized in a freeze dryer for 24 hours. Scaffolds then cross-linked using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (Sigma). The sample was rinsed in distilled water and dried at 37℃ for 4 days.
4
Electrospinning of Formic-Acetic Polycaprolactone Fibers
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PCL (MW 80,000, Sigma) was dissolved in a binary solvent system of formic acid:acetic acid (1:3) at a concentration of 100 mg/mL. An electrospinning apparatus (EC-DIG, IME Technologies, Geldrop, The Netherlands) was used at optimized process conditions to generate continuous, non-woven fibers that were collected onto 18 mm diameter circular glass coverslips attached to a cylindrical drum mandrel (100 mm diameter) rotating at 100 rpm. After electrospinning, coverslips were removed from the mandrel, dried in a fume hood overnight, and stored in an airtight desiccator until use.
5
Fabrication of PCL-based Biocomposites
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For the production of the base material, 400 mg of PCL (Mw ~80,000, Sigma Aldrich, St. Louis, MO, USA) was added to 2 mL of acetic acid (Nacalai Tesque, Kyoto, Japan) and stirred for 12 h. For the composites, 5 mg, 10 mg, and 15 mg (about 1.2%, 2.4%, and 3.6%, respectively) of β-carotene powder (1 µm average particle diameter, Nacalai Tesque, Kyoto, Japan) were added to the solution and stirred for an additional hour.
Printing parameters were chosen from a previous work [33 (link)]: 1 mL of solution was added to a syringe with a 23G needle and injected at a rate of 0.2 μm/s (flow rate: 3.5 × 10−3 mm3/s) under an applied voltage of 10 kV while keeping the metal needle at a fixed distance of 5 cm from the target aluminum foil cathode. The samples were produced under controlled environmental conditions (T = 25 °C and RH = 25–35%).
Printing parameters were chosen from a previous work [33 (link)]: 1 mL of solution was added to a syringe with a 23G needle and injected at a rate of 0.2 μm/s (flow rate: 3.5 × 10−3 mm3/s) under an applied voltage of 10 kV while keeping the metal needle at a fixed distance of 5 cm from the target aluminum foil cathode. The samples were produced under controlled environmental conditions (T = 25 °C and RH = 25–35%).
6
Chiral Hybrid Electrospun Scaffolds
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PCL (MW 80 000, Sigma–Aldrich, United States ) and D(L)PHEG were dissolved in 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) to prepare the D(L)PHEG and PCL mixture. PCL was dissolved at a concentration of 12% w/v at different LPHEG or DPHEG concentrations at 1, 2, 3, and 4 mg/ml. Moreover, 12% w/v PCL/HFIP was prepared as a control. An electrospinning platform was used to generate chiral hybrid scaffolds, which consisted of a grounded collector covered by an aluminum foil, a high-voltage power supply and a syringe driven by a syringe pump. Electrospinning was conducted at an applied voltage of 10.0 kV, solution flow rate of 1.0 ml/h, needle tip-to-collector gap distance of 20 cm, and a collection speed of 200 revolutions per minute. Each of the scaffolds was collected on the aluminum foil after 6 h of electrospinning followed by drying in vacuum.
7
Regenerative Bone Tissue Engineering
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The hDPCs, ADSCs, BMSCs, GFs, and PDLSCs (2 × 106) were mixed with 100 mg of hydroxyapatite/tricalcium phosphate ceramic powder (Zimmer) alone or with rCPNE7 (100 ng/mL) in an 0.5% fibrin gel. The poly ε‐caprolactone (PCL: Mw 80,000, Sigma–Aldrich, Oakville, ON, Canada) was wrapped around the mixed cells and transplanted subcutaneously into immunocompromised mice (NIHbg‐nu‐xid; Harlan Laboratories) for 6 weeks (n = 3, each group).
8
Multifunctional Chitosan-Based Biomaterial
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PCL (Mw 80,000) was obtained from Sigma-Aldrich (Shanghai, China). SF was extracted from Bombyx mori silkworms (Second Silk Company, Zhejiang, China) by our group. AMX (>98%) and MWCNTs were purchased from Adamas (Emeryville, CA, USA) and Aladdin Industrial Company (China), respectively. The carboxylated MWCNTs with lengths of 10–30 μm had inner and outer diameters of approximately 5 and 20 nm, respectively. Hexafluoroisopropanol (HFIP), EDC, and NHS were purchased from Sigma-Aldrich (St. Louis, MO, USA). Escherichia coli was purchased from Shanghai Fuzhong Biotechnology Development Co., Ltd. (Shanghai, China). Luria–Bertani (LB) medium was obtained from Sangon Biotech Co., Ltd. (Shanghai, China). L929 cells were obtained from the Institute of Biochemistry and Cell Biology (The Chinese Academy of Sciences, Shanghai, China). Dulbecco’s modified Eagle’s medium, fetal bovine serum, and glutaraldehyde were purchased from Shanghai Limin Industrial Co., Ltd. (Shanghai, China). Mouse polyclonal anti-CD11b and anti-CD68 antibodies were obtained from Santa Cruz Biotechnology, Inc. (Dallas, TX). Rabbit anti-collagen I antibody was purchased from Sigma-Aldrich (St. Louis, MO).
9
Fabrication of PVA-PCL Graft Scaffold
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The PVA-PCL graft was prepared as previously described. 12 Briefly, a PCL nanofiber sheet was electrospun using a 10% polycaprolactone (PCL, M w 80 000; Sigma-Aldrich, St Louis, MO, USA) solution dissolved in N,N-dimethylformamide, and tetrahydrofuran (3 : 7, w : w) under the following spinning conditions: applied voltage, 20 kV; tip collector distance, 20 cm; flow rate, 1.7 mL h -1 ; and spinning rate, 20 rpm. The resulting sheet was cut (width: 20 mm) and wound around a 1 mm polytetrafluoroethylene (PTFE) axle to form a threelayer sheet. After the PTEF axle was removed, the PCL graft was obtained as a tubular scaffold. Further, a PVA solution containing 33.3% (w/w) ethanol was prepared and slowly added to the PCL graft, after which an empty syringe was used to remove the excess solution remaining on the graft. Finally, the grafts were dried overnight at room temperature (approximately 25 °C).
10
Fabrication of Collagen-PCL Composite Scaffolds
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Reconstituted type I collagen of bovine skin was obtained from Central Leather Research Institute, Adyar, Chennai. PCL (Mw 80,000) was obtained from Sigma Aldrich, USA.
Glacial acetic acid, chloroform, and methanol in analytical grade were obtained from SRL, Mumbai. TiO 2 nanopowder of 20-nm size range was purchased from Sigma Aldrich.
Glacial acetic acid, chloroform, and methanol in analytical grade were obtained from SRL, Mumbai. TiO 2 nanopowder of 20-nm size range was purchased from Sigma Aldrich.
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