BrdU incorporation assays were performed using the Bromodeoxyuridine (BrdU) Incorporation Assay Kit (Sigma-Aldrich, St. Louis, MI, USA) according to the manufacturer’s instructions, except for the inclusion of a post-fixation blocking step in PBS containing 10% FBS.
Bromodeoxyuridine brdu incorporation assay kit
Manufactured by Merck Group
Sourced in United States
The Bromodeoxyuridine (BrdU) Incorporation Assay Kit is a laboratory equipment product that measures cell proliferation. The kit detects and quantifies the incorporation of BrdU, a synthetic nucleoside, into the DNA of dividing cells. This provides a direct measurement of DNA synthesis and can be used to assess cellular proliferation.
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2 protocols using bromodeoxyuridine brdu incorporation assay kit
1
Bromodeoxyuridine Cell Proliferation Assay
2
Comprehensive Analysis of Cell Viability, Apoptosis, and Proliferation
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Cell viability was assessed by methyl thiazolyl tetrazolium (MTT) assay. The cells seeded in 96-well plates were reacted with MTT (10 μl/well; Cwbio, China) at 37°C in the dark. Four hours later, the formazan crystals at the bottom of each well were dissolved by the addition of dimethyl sulfoxide (150 μl/well; Cwbio) at 37°C for 15 min. The absorbance intensity was measured at 490 nm.
Cell apoptosis was measured by the Annexin V-FITC Apoptosis Detection Kit (Univ-bio, China) using a flow cytometer (FACScan; BD).
Cell proliferation was detected using the bromodeoxyuridine (BrdU) incorporation assay kit (Sigma-Aldrich, USA). Briefly, the cells were incubated with BrdU, blocking buffer, a primary antibody against BrdU, secondary antibody, and DAPI. The BrdU-positive cells were counted using a fluorescence microscope (Nikon, Japan).
Cell apoptosis was measured by the Annexin V-FITC Apoptosis Detection Kit (Univ-bio, China) using a flow cytometer (FACScan; BD).
Cell proliferation was detected using the bromodeoxyuridine (BrdU) incorporation assay kit (Sigma-Aldrich, USA). Briefly, the cells were incubated with BrdU, blocking buffer, a primary antibody against BrdU, secondary antibody, and DAPI. The BrdU-positive cells were counted using a fluorescence microscope (Nikon, Japan).
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