Cells were lysed and RNA was isolated using the RNEasy Plus mini kit (Qiagen) or the E.Z.N.A.® HP Total RNA Kit (Omega BioTek). RNA was reverse transcribed to cDNA using the ABI Reverse Transcription Reagents system, using oligo dT for priming (Thermo Scientific). qPCR was performed with cDNA using Power Sybr Green Master Mix (Thermo Scientific) and with the Viia7 quantitative PCR machine (Applied Biosystems). Triplicate technical replicates were performed, outlier replicates (defined as being more than 1 Ct away from other two replicates) were discarded, and relative mRNA was assessed by the ΔΔCt, scaled to the first MYC-OFF timepoint and normalized to β2M (β2 microglobulin). Error bars for qPCR experiments are standard error of the mean (S.E.M.). For U2OS qPCR, only the 4-hour resolution timepoints were used for Replicate 2.
Oligo dt for priming
Manufactured by Thermo Fisher Scientific
Oligo dT for priming is a laboratory product used in the reverse transcription process. It serves as a primer, initiating the synthesis of complementary DNA (cDNA) from mRNA templates.
Automatically generated - may contain errors
Lab products found in correlation
Reverse transcription reagents system, by Thermo Fisher Scientific (2 mentions)
Rneasy plus mini kit, by Qiagen (2 mentions)
Power sybr green master mix, by Thermo Fisher Scientific (2 mentions)
E z n a hp total rna kit, by Omega Bio-Tek (2 mentions)
Viia7 quantitative pcr machine, by Thermo Fisher Scientific (1 mentions)
Viia7, by Thermo Fisher Scientific (1 mentions)
Perfecta sybr green fastmix, by Quantabio (1 mentions)
2 protocols using oligo dt for priming
1
Measuring Gene Expression by qPCR
2
Quantitative PCR Analysis of Gene Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells were lysed and RNA was isolated using the RNEasy Plus mini kit (Qiagen) or the E.Z.N.A. HP Total RNA Kit (Omega BioTek). RNA was reverse transcribed to cDNA using the ABI Reverse Transcription Reagents system, using oligo dT for priming (Thermo Scientific). qPCR was performed with cDNA using Power Sybr Green Master Mix (Thermo Scientific) or PerfeCTa SYBR Green FastMix (QuantaBio) and with the Viia7 or Quant Studio 5 quantitative PCR machines (Applied Biosystems). Triplicate technical replicates were performed for the indicated transcripts (Table 1
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