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Ab17868

Manufactured by Abcam
Sourced in United States

Ab17868 is a primary antibody product manufactured by Abcam. It is a monoclonal antibody targeting a specific protein. The core function of this product is to facilitate the detection and analysis of the target protein in various laboratory applications.

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2 protocols using ab17868

1

Immunodetection Protocols for Cancer Markers

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Western blotting assay was performed according the method reported previously [9 (link)], using anti-URGCP/URG4 (Sigma, HPA02134), anti-p65 (Abcam, ab7970), anti-p84 (Epitomics, EPR5662 (2)), anti-eEF1A1+eEF1A2+eEF1AL3 (Abcam, ab37969, we indicated this antibody as EF-1α), anti-Bcl-2 (Abcam, ab77567) and anti-Caspase-3 antibodies (Abcam, ab17868). The membranes were stripped and re-probed with anti-alpha-Tubulin antibody (Abcam) for a loading control. The protocol of Immunohistochemistry in clinical samples was performed according to previous report [10 (link)]. Anti-URGCP/URG4 antibody (Sigma, HPA02134) was used. The tissue sections were reviewed and scored by two pathologists independently. Immunofluorescence also was performed according to previous report [11 (link)], anti-p65 antibody (Abcam, ab7970) was used.
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2

Comprehensive Protein Analysis of Cell Cycle and Apoptosis

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Western blot analysis was performed as described previously (11 (link)). Equal protein loading was monitored using an anti-GAPDH antibody (60004-1-IG; Proteintech, Rosemount, IL, USA). Primary antibodies against cyclin D1 (ab134175; Abcam), cyclin E1 (ab88259; Abcam), CDK2 (ab32146; Abcam), CDK4 (ab137675; Abcam), CDK6 (ab124821; Abcam), caspase 3 (ab17868; Abcam), polyADP-ribose polymerase (PARP) (ab32064; Abcam), Bax (ab32503; Abcam), matrix metalloprotein (MMP)-3, E-cadherin, and N-cadherin (Cell Signaling Technology, Danvers, MA, USA) were used.
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