Du420 oe

Manufactured by Oxford Instruments

Sourced in United States

The DU420-OE is a laboratory equipment product offered by Oxford Instruments. It is designed for performing specific tasks within a laboratory setting. The core function of the DU420-OE is to facilitate various analytical procedures, but a detailed description of its intended use cannot be provided while maintaining an unbiased and factual approach.

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Lab products found in correlation

Pro em 512 b, by Teledyne (2 mentions) Nrs 4100, by Jasco (1 mentions) Senterra raman spectrometer, by Olympus (1 mentions) Raman spectroscopy, by Bruker (1 mentions) Spcm aqrh 14 fc, by Excelitas (1 mentions)

4 protocols using du420 oe

Confocal Microscopy for Optical Spectroscopy

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The microscope used is an IX 71 (Olympus) with an high numerical aperture objective (MPLAPON 100×/0.95). Optical images are acquired using a CCD camera (PRO EM 512 B, Princeton instruments). During confocal operation, a laser beam is sent through the back port and the emitted fluorescence light is coupled in a multimode fibre (P1-1550A-FC-2, Thorlabs) using a fibre coupler intalled after the tube lens. Spectra are acquired using a spectrometer (MS257, ORIEL Instruments) with a CCD camera (DU420-OE, Andor).
Spectra in Figure 2 are normalized to the transmission of a non-tapered single mode fibre and the blue curves in Figure 4 are averages of both fibre ends. The avalanche photodiodes used in the HBT are SPCM (Perkin Elmer).

Schell A.W., Takashima H., Kamioka S., Oe Y., Fujiwara M., Benson O, & Takeuchi S. (2015). Highly Efficient Coupling of Nanolight Emitters to a Ultra-Wide Tunable Nanofibre Cavity. Scientific Reports, 5, 9619.

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Raman Spectroscopy of Fiber Samples

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Confocal laser Raman spectra were recorded using JASCO NRS-4100 equipped with a DU420-OE charge-coupled device detector (Andor). Samples were placed on a glass slide with a customized chamber made from vinyl adhesive tape, upon which a coverslip was placed and sealed with nail polish. A 532-nm excitation source was used, with the beam focused using a 100× Plan Achromat objective lens (oil), a grating of 900 grooves mm⁻¹, and a 100 μm × 8000 μm slit size. Spectra were acquired from 500 to 2500 cm⁻¹ at 10-mW beam intensity with typical exposures of 3 × 30 s. No sign of sample deterioration was observed under these conditions. All fiber samples were immersed in 0.5 M KPi (pH 5) to ensure a consistent background environment. The collected spectra were calibrated internally to the peak maximum at 1453 cm⁻¹, corresponding to deformation vibrations of CH₂/CH₃ groups. At least eight spectra were collected for each sample, which showed similar results.

Malay A.D., Suzuki T., Katashima T., Kono N., Arakawa K, & Numata K. (2020). Spider silk self-assembly via modular liquid-liquid phase separation and nanofibrillation. Science Advances, 6(45), eabb6030.

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Raman Spectroscopy Analysis of Ettringite

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To distinguish the bound water and hydroxide in the crystal structure of ettringite, further structural analysis was performed by Raman spectroscopy (Bruker Corporation, Billerica, MA, USA) using a Bruker Senterra Raman spectrometer equipped with an Olympus LMPIanFl N 50x lens with the FlexFocus^TM system for confocal depth profiling and an ANDOR DU420-OE with a thermoelectric cooling system as a charge-coupled device (CCD). The green laser (λ = 532 nm) was operated with a total power of 20 mW and a spectral resolution of 3 cm⁻¹ to 5 cm⁻¹. Each spectra was collected with an acquisition time of 5 s per scan, merging five consecutive scans in a range of 50 cm⁻¹ to 1555 cm⁻¹, 1522 cm⁻¹ to 2739 cm⁻¹, and 2705 cm⁻¹ to 3705 cm⁻¹. The grating calibration was controlled by checking the position of the Raman line of a Si standard at 519.9 cm⁻¹ [24 (link)]. The samples were transferred as powder on a glass slide.

Kißling P.A., Lübkemann F., von Bronk T., Cotardo D., Lei L., Feldhoff A., Lohaus L., Haist M, & Bigall N.C. (2021). Influence of Low-Pressure Treatment on the Morphological and Compositional Stability of Microscopic Ettringite. Materials, 14(11), 2720.

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Nanoscale Optical Characterization Setup

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The experimental setup is shown in Fig. 3a. The confocal microscope system is composed of an IX 71 (Olympus) with a high NA objective (MPLAPON 100

\times

/ NA:0.95 Olympus) and a CCD camera (PRO EM 512 B, Princeton instruments). We use a 532 nm laser (Oxxius, LBX-532). The laser spot size is also 355 nm. The light from the laser is reflected by a dichroic mirror onto the nanoflake via an objective lens for optical excitation. The fluorescence from the nanoflakes is collected through the objective lens and the NFBC. After passing through the dichroic mirror, the fluorescence is collected with another objective lens, simplified in the diagram, and coupled to a multimode fibre with a core diameter of about 10

μ

m toward the detection system in Fig. 3b. Also, the fluorescence passing through the NFBC goes directly to the detection system. The detection system consists of a 537 nm edge filter and a 550 nm long-pass filter to cut the excitation light. We also use a 50:50 beam splitter (50:50 BS), single photon counting modules (SPCM-AQRH-14-FC, Excelitas Technologies) to observe the scanning image and the second-order autocorrelation function,

g^{(2)} (τ)

, and a spectrometer (MS257, ORIEL Instruments) with a CCD camera (DU420-OE, Andor).

Tashima T., Takashima H., Schell A.W., Tran T.T., Aharonovich I, & Takeuchi S. (2022). Hybrid device of hexagonal boron nitride nanoflakes with defect centres and a nano-fibre Bragg cavity. Scientific Reports, 12, 96.

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