Images of fruit fly abdomen pigmentation patterns were taken using an Olympus SZX16 Zoom Stereoscope and Olympus DP72 digital camera. Specimens were prepared from four day old flies. Projection images for EGFP-NLS reporter transgene expression were generated with an Olympus Fluoview FV 1000 confocal microscope and software. In each figure panel reporting a pigmentation pattern, gene expression pattern, or CRE activity, a representative image was selected from biological replicate specimens (n≥4). In all figures where comparisons were made between images, each image was processed through the same sequence of modifications using Photoshop CS3 (Adobe).
Szx16 zoom stereoscope
Manufactured by Olympus
The SZX16 Zoom Stereoscope is a versatile optical instrument designed for a wide range of applications. It provides a continuous zoom magnification range, allowing for smooth and precise adjustment of the magnification level. The stereoscope features high-quality optics, ensuring clear and detailed observations.
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Lab products found in correlation
3 protocols using szx16 zoom stereoscope
1
Fruit Fly Abdominal Pigmentation Imaging
2
Assessing Dimorphic Gene Expression
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Images of fruit fly abdomen pigmentation patterns were taken using an Olympus SZX16 Zoom Stereoscope and Olympus DP72 digital camera. Specimens were prepared for 5–10 day old adults. Projection images for EGFP-NLS reporter transgene expression were generated with an Olympus Fluoview FV 1000 confocal microscope and software. The regulatory activities of the t_MSE and t_MSE2 sequences were evaluated at ~90 hours after puparium formation (hAPF), a time point during pupal development when dimorphic expression of tan is first observed in the abdomen [26 (link)]. The regulatory activities for the yellow gene CREs were evaluated at ~85 hAPF, a time point when endogenous sexually dimorphic yellow expression is observed [23 (link)]. In each figure comparing CRE activities, a representative image was selected from replicate specimens (n≥6) and that were processed through the same modifications using Photoshop CS3 (Adobe). In situ hybridization images were taken on the same day using the same microscope and camera, and representative images were selected for Fig 1 and processed through the same modifications.
3
Visualizing Bab1 Expression in Pupal Abdomen
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Images for the specimens taken through the immunohistochemistry protocol, to visualize Bab1 expression in pupal dorsal abdominal tissue, were obtained using an Olympus FV3000 confocal microscope and FV31S-SW imaging software. Samples were imaged with microscope settings as follows: 10% laser power (647 laser), HV between 650 and 700, offset equal to 1, gain equal to 1, aperture set at 180 microns, Z-series step size of 5 microns, and Kalman line averaging set to 2.
Images of adult fruit fly abdomen pigmentation patterns (between two and four days old) and in situ hybridization specimens, were obtained using an Olympus SZX16 zoom stereoscope, running the Olympus cellSens Standard 2.2 software package, with a mounted DP72 digital camera. All samples were imaged at 63X magnification with a 1X objective lens.
Images of adult fruit fly abdomen pigmentation patterns (between two and four days old) and in situ hybridization specimens, were obtained using an Olympus SZX16 zoom stereoscope, running the Olympus cellSens Standard 2.2 software package, with a mounted DP72 digital camera. All samples were imaged at 63X magnification with a 1X objective lens.
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