Determination of the HIV load was performed using the Cobas Ampli prep / Cobas Taqman 96 platform (Roche Diagnostics, Branchburg, New Jersey, USA), per manufacturer’s instructions. The detection limit was <40 copies/ml.
Cobas ampli prep cobas taqman 96 platform
Manufactured by Roche
Sourced in United States
The Cobas Ampli prep / Cobas Taqman 96 platform is a fully automated, high-throughput nucleic acid extraction and real-time PCR system. It is designed for the preparation and analysis of clinical samples.
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Lab products found in correlation
3 protocols using cobas ampli prep cobas taqman 96 platform
1
HIV Viral Load Quantification
2
Quantification of RNA Viral Load
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RNA viral load testing were performed before the start of ART and at 24 and 48 weeks of treatment using an automated RealTime PCR assay:CobasAmpliprep/CobasTaqman 96 platform (Roche Diagnostics, Mannheim, Germany). Plasma samples were analyzed based on the manufacturer’s instruction. The detection limit of the assay was < 40 copies per ml.
3
Viral Load Testing and Resistance Analysis
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Viral load testing was performed at the Bugando Medical Centre clinical laboratory on whole blood collected in 4.5 mL EDTA collection tubes (BD, Franklin Lakes, NJ, USA). Plasma was obtained by centrifuging blood samples at 956 × g for 15 min and was then cryopreserved at −80°C until the time of testing. The viral load estimation was done using the COBAS AmpliPrep/COBAS TaqMan 96 platform (Roche Diagnostics, Branchburg, NJ, USA) following the manufacturer’s instructions.15 Plasma samples with a viral load test result of more than 1000 copies/mL were further analysed for resistance-associated mutations (RAMs).
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