1260 liquid chromatography system

Manufactured by Agilent Technologies

Sourced in United States

The Agilent 1260 Liquid Chromatography System is a high-performance liquid chromatography (HPLC) instrument designed for analytical applications. It is capable of performing separations and quantitative analysis of complex samples. The system features precise pump control, reliable autosampling, and advanced detector capabilities to enable accurate and reproducible results.

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Lab products found in correlation

Gf254, by Qingdao Haiyang Chemical (12 mentions) Avance 3 600 mhz spectrometer, by Bruker (10 mentions) Silica gel, by Qingdao Haiyang Chemical (7 mentions) Fourier transform infrared spectrometer, by Shimadzu (6 mentions) Zorbax sb c18 column, by Agilent Technologies (5 mentions) Sepa 300 polarimeter, by Horiba (4 mentions) C 605, by Büchi (4 mentions) C 615, by Büchi (4 mentions) Sb c18 column, by Agilent Technologies (4 mentions) Sepacore system, by Büchi (3 mentions) Agilent 6200 q tof ms system, by Agilent Technologies (3 mentions) Q exactive orbitrap ms system, by Thermo Fisher Scientific (3 mentions) 6200 q tof ms system, by Thermo Fisher Scientific (3 mentions) Sephadex lh 20, by GE Healthcare (3 mentions) Uh5300, by Hitachi (2 mentions) Autospec premier p776 mass spectrometer, by Waters Corporation (2 mentions) Uv 2401pc uv visible recording spectrophotometer, by Shimadzu (2 mentions) Dad detector, by Agilent Technologies (2 mentions) Ultimate 3000 rslc uplc system, by Thermo Fisher Scientific (2 mentions) Avance 3 600, by Bruker (2 mentions)

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24 protocols using 1260 liquid chromatography system

Analytical Techniques for Compound Characterization

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Optical rotations were measured on
a Rudolph Autopol IV polarimeter (Hackettstown). Ultraviolet (UV)
spectra were obtained using a double beam spectrophotometer (UH5300,
Hitachi High-Technologies, Tokyo, Japan). Infrared (IR) spectra were
obtained on a Shimadzu Fourier transform infrared spectrometer using
KBr pellets. High-resolution electrospray ionization mass spectra
(HRESIMS) were measured on an Exactive Orbitrap mass spectrometer
with a HESI ion source (ThermoFisher Scientific, Bremen, Germany).
Nuclear magnetic resonance (NMR) spectra were recorded with a Bruker
Avance III 600 MHz spectrometer (Bruker, Karlsruhe, Germany) with
tetramethylsilane (TMS) as an internal standard. Circular dichroism
(CD) spectra were measured with an Applied Photophysics spectrometer
(Chirascan, New Haven). Silica gel (200–300 mesh), Sephadex
LH-20 (GE Healthcare), and reverse phase (RP)-18 gel (20–45
μm, FuJi) were used for column chromatography. Preparative or
semipreparative high-performance liquid chromatography (HPLC) was
performed on an Agilent 1260 liquid chromatography system equipped
with Zorbax SB-C18 columns (5 μm, 9.4 mm × 150 mm or 21.2
mm × 150 mm). Chiral separation was carried out on a Chiralpak
AD-H chiral column (5 μm, 250 × 4.6 mm, Daicel).

Dai Q., Zhang F.L., Du J.X., Li Z.H., Feng T, & Liu J.K. (2020). Illudane Sesquiterpenoids from Edible Mushroom Agrocybe salicacola and Their Bioactivities. ACS Omega, 5(34), 21961-21967.

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Characterization of Organic Compounds

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Melting points were measured on a WRX-4 apparatus. Optical rotations were measured with a Horiba SEPA-300 polarimeter. IR spectra were obtained with a Tenor 27 spectrophotometer using KBr pellets. 1D and 2D spectra were run on a Bruker Avance III 600 MHz spectrometer with TMS as an internal standard. Chemical shifts (δ) were expressed in ppm with reference to the solvent signals. Mass spectra were recorded on an Agilent 6200 Q-TOF mass spectrometry system. Column chromatography (CC) was performed on silica gel (200–300 mesh), RP-18 gel (20–45 μm), and Sephadex LH-20. Medium pressure liquid chromatography (MPLC) was performed on a Biotage One equipment packed with RP-18 gel columns. Preparative high performance liquid chromatography (prep-HPLC) was performed on an Agilent 1260 liquid chromatography system equipped with Zorbax SB-C₁₈ columns (5 μM, 9.4 × 250 mm) and a DAD detector. Fractions were monitored by TLC (GF 254), and spots were visualized by heating silica gel plates sprayed with 10% H₂SO₄ in EtOH.

Zhang X.F., Du J.X., Teng S.Q., Liu H., He J., Feng T, & Liu J.K. (2024). Carpesabrolide A, a novel meroterpenoid with anti-inflammatory activity from Carpesium abrotanoides. RSC Advances, 14(16), 11002-11006.

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Comprehensive Analytical Characterization Protocol

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Optical rotations were obtained on a JASCO P-1020 digital polarimeter (Horiba, Kyoto, Japan). UV spectra were recorded on a Shimadzu UV-2401PC UV–visible recording spectrophotometer (Shimadzu, Kyoto, Japan). 1D and 2D NMR spectra were obtained on a Bruker Avance III 600 MHz spectrometer (Bruker Corporation, Karlsruhe, Germany). HRESIMS were recorded on an Agilent 6200 Q-TOF MS system (Agilent Technologies, Santa Clara, CA, USA). HREIMS were obtained on a Waters Autospec Premier P776 mass spectrometer. Single crystal X-ray diffraction was performed on an APEX II DUO spectrophotometer (Bruker AXS GmbH, Karlsruhe, Germany). Sephadex LH-20 (Amersham Biosciences, Uppsala, Sweden) and silica gel (Qingdao Haiyang Chemical Co., Ltd) were used for column chromatography (CC). Medium pressure liquid chromatography (MPLC) was performed on a Büchi Sepacore System equipped with pump manager C-615, pump modules C-605 and fraction collector C−660 (Büchi Labortechnik AG, Flawil, Switzerland), and columns packed with Chromatorex C-18 (40–75 μm, Fuji Silysia Chemical Ltd., Kasugai, Japan). Preparative high performance liquid chromatography (prep-HPLC) were performed on an Agilent 1260 liquid chromatography system equipped with Zorbax SB−C18 column (particle size 5 μm, dimension 9.4 mm × 150 mm, flow rate 8 mL/min, respectively) and a DAD detector (Agilent Technologies, Santa Clara, CA, USA).

Chen H.P., Jiang M.Y., Zhao Z.Z., Feng T., Li Z.H, & Liu J.K. (2017). Vibralactone Biogenesis-Associated Analogues from Submerged Cultures of the Fungus Boreostereum vibrans. Natural Products and Bioprospecting, 8(1), 37-45.

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YQJPF: Multiherbal Decoction Characterization

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YQJPF was provided by Nanjing University of Chinese Medicine, Nanjing Hospital Affiliated to Nanjing University of Chinese Medicine. YQJPF was prepared from nine commonly used Chinese herbal medicines (Table 1). All the herbal constituents were obtained from the Beijing Tong Ren Tang Co. Ltd. (Beijing, China). Huangqi, Taizishen, Baizhu, Chenpi, Danggui, Fulin, Huangqin, and Gancao were mixed in the ratio 30:30:30:10:15:15:3:10:10.
Then, all herbs were decocted twice and the decoction liquids were concentrated to a density of 2.86 g/ml and was stored at 4°C. For quality control, HPLC analysis was used as previously described (Yao et al., 2018 (link)). YQJPF were assessed using an Agilent 1,260 liquid chromatography system (United Kingdom). Briefly, 10 μl YQJPF solution was injected into an apparatus with an auto sampler. Chromatographic separation was implemented at a flow rate of 1 ml/min with an Agilent C18 column (4.6 mm × 250 mm, 5 μm). The separation phase was composed of 0.2% phosphoric acid (A) and acetonitrile (B). The linear concentration solution gradually increases from 2 to 72% of solvent B over the course of 45 min. The separation temperature was 40°C, with a detection wavelength of 254 nm. The results are shown in Figure 1.

Wang F., Tang L., Liang B., Jin C., Gao L., Li Y., Li Z., Shao J., Zhang Z., Tan S., Zhang F, & Zheng S. (2021). Yi-Qi-Jian-Pi Formula Suppresses RIPK1/RIPK3-Complex-Dependent Necroptosis of Hepatocytes Through ROS Signaling and Attenuates Liver Injury in Vivo and in Vitro. Frontiers in Pharmacology, 12, 658811.

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Spectroscopic Analysis of Molecular Compounds

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Optical rotations were measured on a Rudolph Autopol IV polarimeter. UV spectra were obtained on a UH5300 UV-VIS Double Beam Spectrophotometer. IR spectra were obtained by using a Shimadu Fourier Transform Infrared spectrometer with KBr pellets. NMR spectra were acquired with a Bruker Avance III 600 instrument. ECD spectra were recorded with an Applied Photophysics Chirascan-Plus spectrometer. High resolution electrospray ionization mass spectra (HRESIMS) were recorded on a LC-MS system consisting of a Q Exactive™ Orbitrap mass spectrometer with an ESI ion source used in ultra-high resolution mode (140 000, at m/z 200) and a Dionex UltiMate 3000 RSLC UPLC system. Silica gel (200–300 mesh and 500–800 mesh), RP-18 gel (40–75 μm) and Sephadex LH-20 were used for column chromatography. Preparative HPLC was performed on an Agilent 1260 liquid chromatography system with a Zorbax SB-C18 (5 μm, 9.4 × 150 mm) column and a DAD detector.

Yi X.W., He J., Sun L.T., Liu J.K., Wang G.K, & Feng T. (2021). 3-Decalinoyltetramic acids from kiwi-associated fungus Zopfiella sp. and their antibacterial activity against Pseudomonas syringae. RSC Advances, 11(31), 18827-18831.

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Spectroscopic Characterization of Organic Compounds

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Melting points were obtained on an X-4 micro melting point apparatus. Optical rotations were measured with a Horiba SEPA-300 polarimeter. IR spectra were obtained with a Tenor 27 spectrophotometer using KBr pellets. 1D and 2D spectra were run on a Bruker Avance III 600 MHz spectrometer with TMS as an internal standard. Chemical shifts (δ) were expressed in ppm with reference to the solvent signals. Mass spectra were recorded on an Agilent 6200 Q-TOF MS system. Column chromatography (CC) was performed on silica gel (200–300 mesh, Qingdao Marine Chemical Ltd., Qingdao, People’s Republic of China), RP-18 gel (20–45 µm, Fuji Silysia Chemical Ltd., Japan), and Sephadex LH-20 (Pharmacia Fine Chemical Co., Ltd., Sweden). Medium Pressure Liquid Chromatography (MPLC) was performed on a Büchi Sepacore System equipping with pump manager C-615, pump modules C-605 and fraction collector C-660 (Büchi Labortechnik AG, Flawil, Switzerland), and columns packed with RP-18 gel. Preparative High Performance Liquid Chromatography (prep-HPLC) was performed on an Agilent 1260 liquid chromatography system equipped with Zorbax SB-C18 columns (5 μm, 9.4 mm × 150 mm or 21.2 mm × 150 mm) and a DAD detector. Fractions were monitored by TLC (GF 254, Qingdao Haiyang Chemical Co., Ltd. Qingdao), and spots were visualized by heating silica gel plates sprayed with 10% H₂SO₄ in EtOH.

Shi C., Peng Y.L., He J., Li Z.H., Liu J.K, & Feng T. (2021). Structures, Chemical Conversions, and Cytotoxicity of Tricholopardins C and D, Two Tricholoma Triterpenoids from the Wild Mushroom Tricholoma pardinum. Natural Products and Bioprospecting, 11(2), 235-241.

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Analytical Techniques for Natural Product Characterization

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Optical rotations were measured on a Jasco-P-1020 polarimeter. IR spectra were obtained using a Bruker Tensor 27 FT-IR spectrometer with KBr pellets. NMR spectra were acquired with a Bruker DRX-600 with tetramethylsilane (TMS) used as an internal standard. HRESIMS were recorded on an API QSTAR Pulsar spectrometer. Silica gel (200–300 mesh), Sephadex LH-20 and RP-18 gel (20–45 µm) were used for column chromatography (CC). Medium pressure liquid chromatography (MPLC) was performed on a Biotage system. Preparative high performance liquid chromatography (prep-HPLC) was performed on an Agilent 1260 liquid chromatography system equipped with Zorbax SB-C18 columns (5 μm, 9.4 mm × 150 mm or 21.2 mm × 150 mm) and a DAD detector. Fractions were monitored by TLC and spots were visualized by heating silica gel plates immersed in H₂SO₄ in EtOH, in combination with the Agilent 1200 series HPLC system (Eclipse XDB-C18 column, 5 μm, 4.6 × 150 mm).

Yu X., Duan K.T., Wang Z.X., Chen H.P., Gan X.Q., Huang R., Li Z.H., Feng T, & Liu J.K. (2017). Anemhupehins A–C, Podocarpane Diterpenoids from Anemone hupehensis. Natural Products and Bioprospecting, 8(1), 31-35.

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Spectroscopic Characterization of Compounds

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Optical rotations (ORs)
were measured using a Horiba SEPA-300 polarimeter. Infrared (IR) spectra
were obtained with a Tenor 27 spectrophotometer using KBr pellets.
One-dimensional (1D) and two-dimensional (2D) nuclear magnetic resonance
(NMR) spectra were recorded using a Bruker Avance III 600 MHz spectrometer
with tetramethylsilane as an internal standard. Chemical shifts (δ)
were expressed in ppm with reference to the solvent signals. High-resolution
electrospray ionization mass spectra (HRESIMS) were recorded on an
Agilent 6200 Q-TOF MS system or a Thermo Scientific Q Exactive Orbitrap
MS system. Column chromatography (CC) was performed on silica gel
(200–300 mesh, Qingdao Haiyang Chemical Co., Ltd., China),
reverse phase-C₁₈ gel (20–45 μm, Fuji), and
Sephadex LH-20 (GE Healthcare, Sweden). Medium pressure liquid chromatography
(MPLC) was performed using Biotage SP1 equipment and columns packed
with RP-C₁₈ gel. Preparative high-performance liquid chromatography
(prep-HPLC) was performed using an Agilent 1260 liquid chromatography
system equipped with Zorbax SB-C18 columns (5 μm, 9.4 mm ×
150 mm, or 21.2 mm × 150 mm) and a diode array detector (DAD).
Fractions were monitored using the DAD detector or thin layer chromatography
(TLC) (GF 254, Qingdao Haiyang Chemical Co., Ltd., China).

Yang H.X., Ma J.T., He J., Li Z.H., Huang R., Feng T, & Liu J.K. (2021). Pardinumones A–D: Antibacterial Polyketide–Amino Acid Derivatives from the Mushroom Tricholoma pardinum. ACS Omega, 6(38), 25089-25095.

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Spectroscopic Analysis of Natural Compounds

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UV spectra were afforded with a UH5300 UV-VIS Double Beam Spectrophotometer. ECD spectra were obtained on a Chirascan CD spectrometer (Applied Photophysics, London, UK). HRESIMS spectra were recorded on a Q Exactive Obitrap mass spectrometer (ThermoFisher Scientific, Waltham, MA, USA) and UPLC-ESI-Q-TOF-MS (1290 UPLC-6540, Agilent Technologies Inc., Palo Alto, CA, USA) HRMS spectrometer. IR spectra were obtained with a Shimadzu Fourier Transform Infrared Spectrometer using KBr pellets. NMR spectra were recorded on a Bruker Avance III 600 MHz spectrometer with TMS as an internal standard. Chemical shifts (δ) were expressed in ppm with reference to the solvent signals. Column chromatography (CC) was performed on silica gel (200–300 mesh, Qingdao Marine Chemical Ltd., Qingdao, China) and Sephadex LH-20 (Pharmacia Fine Chemical Co., Ltd., Uppsala, Sweden). Medium Pressure Liquid Chromatography (MPLC) was performed on a Biotage SP1 System and columns packed with RP-18 gel. Preparative high-performance liquid chromatography (prep-HPLC) was performed on an Agilent 1260 liquid chromatography system equipped with Zorbax SB-C18 columns (Agilent, 5 μm, 9.4 mm × 150 mm) and a DAD detector. Fractions were monitored by TLC (GF 254, Qingdao Haiyang Chemical Co., Ltd., Qingdao, China).

Long H., Zhou S., Li L., Li J, & Liu J. (2023). Two New Compounds from the Fungus Xylaria nigripes. Molecules, 28(2), 508.

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Characterization of Natural Products

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Optical rotations were obtained on a JASCO P-1020 digital polarimeter (Horiba, Kyoto, Japan). UV spectra were recorded on a Shimadzu UV-2401PC UV–Visible recording spectrophotometer (Shimadzu, Kyoto, Japan). A Tenor 27 spectrophotometer (Bruker Optics GmbH, Ettlingen, Germany) was used for scanning IR spectroscopy using KBr pellets. 1D and 2D NMR spectra were obtained on a Bruker Avance III 600 MHz spectrometer (Bruker Corporation, Karlsruhe, Germany). HRESIMS were recorded on an Agilent 6200 Q-TOF MS system (Agilent Technologies, Santa Clara, CA, USA). Sephadex LH-20 (Amersham Biosciences, Uppsala, Sweden) and silica gel (Qingdao Haiyang Chemical Co., Ltd., Qingdao, China) were used for column chromatography (CC). Medium Pressure Liquid Chromatography (MPLC) was performed on a Büchi Sepacore System equipped with pump manager C-615, pump modules C-605 and fraction collector C-660 (Büchi Labortechnik AG, Flawil, Switzerland), and columns packed with Chromatorex C-18 (dimensions 450 mm × i.d. 14 mm, particle size: 40–75 μm, Fuji Silysia Chemical Ltd., Kasugai, Japan). Preparative high performance liquid chromatography (prep-HPLC) were performed on an Agilent 1260 liquid chromatography system equipped with a Zorbax SB-C18 column (particle size 5 μm, dimensions 150 mm × i.d. 9.4 mm, flow rate 7 ml min⁻¹) and a DAD detector (Agilent Technologies, Santa Clara, CA, USA).

Chen H.P., Zhao Z.Z., Li Z.H., Feng T, & Liu J.K. (2018). Seco-tremulane Sesquiterpenoids from the Cultures of the Medicinal Fungus Irpex lacteus HFG1102. Natural Products and Bioprospecting, 8(2), 113-119.

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