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Fitc cd8α

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FITC-CD8α is a fluorescent-labeled antibody that binds to the CD8α subunit of the CD8 receptor, which is expressed on the surface of certain T cells. It is commonly used in flow cytometry applications to identify and quantify CD8-positive T cells in biological samples.

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Lab products found in correlation

Cd3 pe, by Thermo Fisher Scientific (1 mentions) Annexin 5 apc, by BD (1 mentions) 7 aad, by BD (1 mentions) Cyan adp, by Beckman Coulter (1 mentions) Cd3 percp, by Thermo Fisher Scientific (1 mentions) Ifnγ elispot, by Mabtech (1 mentions)

Spelling variants of this product

Anti–CD8α-FITC (2 citations) Anti-CD8α-FITC (53-6.7; (2 citations)

2 protocols using fitc cd8α

1

Detecting Cell Apoptosis by Flow Cytometry

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Splenocytes were treated with anti-CD16/CD32 and cell surface antigens were labeled with PE-CD3 and FITC-CD8α (eBioscience). Cells were then labelled with APC-AnnexinV and 7-AAD (BD Pharmingen) for 15 minutes and data was acquired on the Cyan-ADP (Beckman Coulter) within an hour.
Ananth A.A., Tai L.H., Lansdell C., Alkayyal A.A., Baxter K.E., Angka L., Zhang J., Tanese de Souza C., Stephenson K.B., Parato K., Bramson J.L., Bell J.C., Lichty B.D, & Auer R.C. (2016). Surgical Stress Abrogates Pre-Existing Protective T Cell Mediated Anti-Tumor Immunity Leading to Postoperative Cancer Recurrence. PLoS ONE, 11(5), e0155947.
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2

Quantifying Antigen-Specific CD8+ T cells

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Splenocytes were treated with anti-CD16/CD32 and labelled with PE-conjugated DCT180-188 peptide-loaded MHC-I tetramer (Baylor College), PerCP-CD3, and FITC-CD8α (eBioscience) for 30 minutes in FACS buffer (1% PBS, 5% fetal calf serum, 2.5% NaZ). For ELISpot analyses, the exact number of DCT-MHC-I tetramer+/CD8+ T cells were calculated from flow cytometry proportion results and added to an IFNγ ELISpot following manufacturer’s instructions (Mabtech).
Ananth A.A., Tai L.H., Lansdell C., Alkayyal A.A., Baxter K.E., Angka L., Zhang J., Tanese de Souza C., Stephenson K.B., Parato K., Bramson J.L., Bell J.C., Lichty B.D, & Auer R.C. (2016). Surgical Stress Abrogates Pre-Existing Protective T Cell Mediated Anti-Tumor Immunity Leading to Postoperative Cancer Recurrence. PLoS ONE, 11(5), e0155947.
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