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Anti tip60

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Anti-Tip60 is a primary antibody used to detect the Tip60 protein in biological samples. Tip60 is a histone acetyltransferase involved in various cellular processes, such as DNA repair and chromatin remodeling. The antibody can be used in techniques like Western blot, immunoprecipitation, and immunofluorescence to study the expression and localization of Tip60.

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Lab products found in correlation

Anti gfp, by Merck Group (1 mentions) Vectashield, by Vector Laboratories (1 mentions) Anti ha, by Abcam (1 mentions) Anti sirt3, by Abcam (1 mentions) Anti flag, by Abcam (1 mentions) Surebeads protein a g magnetic beads, by Bio-Rad (1 mentions) Ls6500, by Beckman Coulter (1 mentions) Protein g dynabead, by Thermo Fisher Scientific (1 mentions) Anti cbp, by Abcam (1 mentions) Anti p300, by Abcam (1 mentions) Anti pcaf, by Abcam (1 mentions) Anti gapdh antibody, by Proteintech (1 mentions) Cycloheximide (chx), by MedChemExpress (1 mentions) Anti acetyl β catenin k49, by Cell Signaling Technology (1 mentions) D4476, by MedChemExpress (1 mentions) Mg149, by MedChemExpress (1 mentions) Nu9056, by MedChemExpress (1 mentions) Anti ck1δ, by Santa Cruz Biotechnology (1 mentions) Anti β catenin, by Santa Cruz Biotechnology (1 mentions) Clonexpress ultra one step cloning kit, by Vazyme (1 mentions)

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Tip60 (3 citations)

5 protocols using anti tip60

1

Immunostaining of Drosophila Brains

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Adult Drosophila brains were dissected in PBS, fixed in 4% paraformaldehyde in PBS, washed three times in PBS containing 0.1% Triton X-100, blocked for 1 hr at room temperature (RT) in PBT containing 5% normal goat serum, and incubated with primary antibodies in blocking solution overnight at 4°C. Anti-Tip60 (1:400) was obtained from Abcam, anti-Fasciclin (mAb1D4; 1:10) was obtained from the Developmental Studies Hybridoma Bank (University of Iowa). Anti-GFP (1:100) was obtained from Millipore. Samples were washed three times in PBST at RT, and secondary antibodies (Jackson Immunoresearch) were applied in blocking solution for 2 hr at RT. After washing three times in PBS, samples were mounted in Vectashield (Vector Laboratories).
Xu S., Panikker P., Iqbal S, & Elefant F. (2016). Tip60 HAT Action Mediates Environmental Enrichment Induced Cognitive Restoration. PLoS ONE, 11(7), e0159623.
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2

SIRT1-7, TIP60, and GPAT3 Interaction Analysis

Cited 1 time
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The open reading frame of human SIRT1‐7, TIP60, and GPAT3 (Miaolingbio) were subcloned into pcDNA3.1‐3×flag or pcDNA3.1‐HA vectors. Transfected cells were lysed with NP‐40 lysis buffer (Biosharp, Anhui, China). Anti‐AcGPAT3 were custom‐made from GL (GL Biochem, Shanghai, China) and applied for IP and Co‐IP assays. The immune precipitants eluted from SureBeads™ Protein A&G Magnetic Beads (Bio‐Rad Inc., Hercules, CA, USA) were examined by immunoblotting as described.
32 (link) Anti‐rabbit and mouse IgG (Beyotime) were used as negative controls. Anti‐TIP60, anti‐SIRT3, anti‐GPAT3, anti‐Flag, and anti‐HA (1:1000) were obtained from Abcam (Cambridge, MA, USA).
Wang Y., Xu C., Yang X., Liu X., Guo Z., Lin X., Li L, & Huang Z. (2024). Glycerol‐3‐phosphate acyltransferase 3‐mediated lipid droplets accumulation confers chemoresistance of colorectal cancer. MedComm, 5(2), e486.
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3

Assaying HAT and HDAC Activities

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HNPCs nuclear extracts were prepared as described previously [26 (link)]. HAT activity and HDAC activity assays were done using nuclear extracts following the protocols of the manufacturer (BioVision Biotechnology). For HAT activity assays, immunoprecipitations were done using anti-p300, anti-Tip60, anti-CBP, and anti-PCAF (Abcam, Cambridge, UK) with hNPCs nuclear extracts. Precleared nuclear extract was incubated with antibodies overnight with Protein G dynabeads (Thermo, Waltham, Massachusetts, USA) at 4°C. All samples were counted with a multipurpose scintillation counter, LS 6500 (Beckman, California, USA).
Huang Y., Chen J., Jiang T., Zhou Z., Lv B., Yin G, & Fan J. (2017). Gallic acid inhibits the release of ADAMTS4 in nucleus pulposus cells by inhibiting p65 phosphorylation and acetylation of the NF-κB signaling pathway. Oncotarget, 8(29), 47665-47674.
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4

Regulation of β-Catenin Acetylation

Cited 1 time
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SR3029, D4476, MG149, NU9056, MG132 and cycloheximide (CHX) were purchased from MedChemExpress (MCE, Monmouth Junction, NJ, USA). The following primary antibodies were used: anti-CK1δ, anti-β-catenin, anti-p300, anti-acetyl-lysine, IgG (Santa Cruz Biotechnology, Heidelberg, Germany), anti-CBP, anti-acetyl-β-catenin (K49), anti-phospho-β-catenin (S45), anti-Flag, anti-V5, anti-CK1ϵ, anti-GFP, anti-mouse IgG (Cell Signaling Technology, Danvers, MA), anti-Tip60 (Abcam, Cambridge, MA, USA), and anti-GAPDH antibody (Proteintech, Chicago, IL, USA). The Tip60-Flag, PCAF-Flag, p300-Flag and pEGFP-N1 plasmids were purchased from Vigenebio (Weizhen, Shandong, China). The SuperTopFlash reporter plasmid was provided by Karl Willert (University of California at San Diego, La Jolla, CA, USA). The expression plasmids encoding β-catenin, pCMXβgal (β galactosidase, β-gal), CK1α-V5, CK1δ-V5, CK1ϵ-V5, CK1α-Flag, CK1δ-Flag, CK1ϵ-Flag, CK1γ-Flag have been described previously (35 (link), 36 (link)). For the construction of GFP-tagged CK1δ, the cDNAs encoding human CK1δ was amplified by PCR and subcloned into the BamHI/EcoRI site of pEGFP-N1 vector using ClonExpress Ultra One Step Cloning Kit (Vazyme, Nanjing, China). The primer sequences used are as follows: CK1δ-GFP-sense, 5’-CTCGAGCTCAAGCTTCATGGAGCTGAGAGTCGGGAAC-3’; CK1δ-GFP-antisense, 5’-CTCACCATAAGGTGGCGACCGGTGTAGGTGCGTCGTG.
Ning J., Sun Q., Su Z., Tan L., Tang Y., Sayed S., Li H., Xue V.W., Liu S., Chen X, & Lu D. (2022). The CK1δ/ϵ-Tip60 Axis Enhances Wnt/β-Catenin Signaling via Regulating β-Catenin Acetylation in Colon Cancer. Frontiers in Oncology, 12, 844477.
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5

Western Blot Analysis of Protein Samples

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For Western blot analysis, cells were lysed in RIPA buffer containing protease-inhibitor mixture (Roche) and the Bradford method (Bio-rad) was applied to measure protein concentration. Cell lyses were boiled in 2×SDS loading buffer for 5 min and prepared for gel running. Antibodies used in Western blots were, anti-p53, anti-TUBULIN, anti-Myc, anti-GAPDH (Santa Cruz); anti-K120Ac-p53, anti-TIP60 (Abcam); anti-HA (Milipore); anti-cleaved-PARP, anti-TRRAP, anti-p400, anti-DMAP1 and anti-RUVBL1 (Cell Signaling) antibodies.
Fang X., Lu G., Ha K., Lin H., Du Y., Zuo Q., Fu Y., Zou C, & Zhang P. (2017). Acetylation of TIP60 at K104 is essential for metabolic stress-induced apoptosis in cells of hepatocellular cancer. Experimental cell research, 362(2), 279-286.
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