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2 protocols using ammonium iron 2 sulfate

1

Antioxidant Capacity Evaluation Protocol

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Tocopherol standards and malondialdehyde were purchased from Merck Ltd. (Darmstadt, Germany). Ammonium iron (II) sulfate, thiobarbituric acid, trichloroacetic acid, hydrochloric acid (37%), and glacial acetic acid were purchased from Panreac (Barcelona, Spain). Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) was purchased from Glentham Life Sciences (Corsham, UK). Dichloromethane and isooctane were obtained from Carlo Erba (Vaul de Reuil, France). Ammonium thiocyanate and chloroform were purchased from Penta (Prague, Czech Republic). Cyclohexane and p-anisidine were obtained from Sigma-Aldrich (St. Louis, Burlington, MA, USA). Ethanol (99.8%) was bought from Fischer Scientific (Loughborough, UK). Hydrogen peroxide (35%) was obtained from Chemco (Malsch, Germany).
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2

Quantification of Fatty Acid Methyl Esters

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Fatty acid methyl esters (FAME) were purchased from Nu-Check (Elysian, MN, USA): Stearate, Oleate, Linoleate and Linolenate. Mixture of plant sterols (PS) (54 % sitosterol, 30 % campesterol, 15 % stigmasterol), 5α-cholestane, heptadecanoic acid and ammonium thyocianate were purchased from Sigma-Aldrich Chemical (Steinheim, Germany). 19hydroxycholesterol was obtained from Steraloids (Wilton, NH, USA). Tri-sil ® reagent was obtained from Thermo-Scientific (Rockford, IL, USA). Hexane, heptane, acetone, chloroform, ethyl acetate, butanol, methanol, 2-propanol, hydrochloric acid, ammonium iron (II) sulfate and barium chloride, were obtained from Panreac (Barcelona, Spain).
Strata NH 2 (55 µm, 70 A) 500 mg / 3 mL Solid Phase Extraction cartridges were obtained from Phenomenex (Torrance, USA).
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