Is4000mm

The in vivo tumor targeting ability of A15-CUR LPs was evaluated by non-invasive optical imaging systems. Tumor-bearing mice models were built according to the previous report. Briefly, 1×10⁷ DU145 cells were suspended in 200 μL DMEM medium and injected into the left hindlimb flank of mice. DiD (NIRF dye, 50 nM) were co-loaded into A15-CUR LPs as described earlier. When tumor volume reached ~100–150 mm³, DiD-CUR, DiD-CUR LPs and DiD-A15-CUR LPs in 100 µL PBS were injected intravenously via the tail vein into tumor-bearing mice. At different time points (6 and 12 hrs), mice were anesthetized by intraperitoneal injection of pentobarbital (35 mg/kg) and scanned using the imaging system IS4000MM (Kodak) with an excitation bandpass filter at 625 nm and an emission at 700 nm. Exposure time was 30 s per image.

The in vivo tumor targeting ability of RGD-PEG₃₅₀₀-DSPE GEM LPs was evaluated by non-invasive optical imaging systems. Tumor-bearing mice models were built according to the previous report. Briefly, 1×10⁹ SKOV3 cells were suspended in 200 μL DMEM medium and injected into the right hindlimb flank of mice. DiD (NIRF dye, 50 nM) was co-loaded into RGD-PEG₃₅₀₀-DSPE GEM LPs as described above. When tumor volume reached ~100–150 mm³, DiD-GEM, DiD-GEM LPs and DiD-RGD-PEG₃₅₀₀-DSPE GEM LPs in 100 µL PBS were injected intravenously via the tail vein in tumor bearing mice. At different time points (12 and 24 h), mice were anesthetized by intraperitoneal injection of pentobarbitol (35 mg/kg), and scanned using the imaging system IS4000MM (Kodak) with an excitation bandpass filter at 625 nm and an emission at 700 nm. Exposure time was 30 s per image.