Voriconazole, voriconazole N-Oxide, and fluconazole were purchased from Melone Biotechnology Co., Ltd. (Beijing, China). The UPLC® BEH C18 column (2.1 mm × 50 mm, 1.7 µm) used for liquid chromatography was supplied by the Waters (Millipore, Bedford, MA, USA). NADPH was provided by Promega (Madison, WI, USA). P450 Cytochrome b5 and recombinant human CYP2C19 enzymes (the wild-type CYP2C19.1 and 30 CYP2C19 variants) effectively generated in insect cell Sf21 were gifted by Beijing Hospital, National Health and Family Planning Commission of Republic of China.17 (link) Methanol and acetonitrile were of liquid chromatography grade and were obtained from Merck Chemicals Co., Ltd. (Darmstadt, Germany). Formic acid and other chemicals were all of analytical grade and were prepared from Sigma-Aldrich Co. (St. Louis, MO, USA) and Chemical Industries (Beijing, China), respectively. Ultrapure water used for the experiment was supplied from Milli-Q water purification system (Millipore, Bedford, MA, USA).
Nadph
Manufactured by Promega
Sourced in United States
NADPH is a coenzyme derived from the vitamin niacin. It plays a crucial role in cellular metabolism by serving as a reducing agent in various enzymatic reactions, particularly in the biosynthesis of lipids and nucleic acids.
Automatically generated - may contain errors
Lab products found in correlation
Uplc beh c18 column, by Waters Corporation (1 mentions)
Milli q water purification system, by Merck Group (1 mentions)
Formic acid, by Merck Group (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Sorafenib, by Merck Group (1 mentions)
Sorafenib n oxide, by Merck Group (1 mentions)
Hplc grade solvents, by Thermo Fisher Scientific (1 mentions)
Analytical grade reagents, by Merck Group (1 mentions)
2 protocols using nadph
1
CYP2C19 Enzymatic Profiling of Antifungal Drugs
2
Sorafenib Metabolism by CYP3A5 Variants
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Sorafenib and Sorafenib N-oxide were purchased from Sigma-Aldrich (St. Louis, MO, USA). The ultra-performance liquid chromatography (UPLC)@BEH C18 column (2.1 mm × 50 mm, 1.7 μm) was purchased from Millipore (Bedford, MA, USA). NADPH was obtained from Promega (Madison, WI, USA). Recombinant human cDNA encoding wild-type (CYP3A5*1) gene or each of six variant genes (CYP3A5*2, CYP3A5*3A, CYP3A5*3C, CYP3A5*4, CYP3A5*5, CY-P3A5*6, and CYP3A5*7) was purchased from Wuhan Bafeier Biological (Wuhan, China). Sorafenib N-oxide as internal standard was purchased from Sigma-Aldrich (St Louis, MO, USA). HPLC-grade solvents were obtained from Fisher Scientific (Pittsburgh, PA, USA), and analytical grade reagents were obtained from Merck Chemicals (Darmstadt, Germany). Rabbit anti-human CYP3A5 IgG primary antibody was obtained from Sigma-Aldrich, and the secondary goat anti-rabbit IgG-HRP-conjugated antibody was purchased from Shanghai Gu Ge Biology (Shanghai, China).
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