Phenix imaging film

ATP, Mops, HEPES, Tris, MgCl₂, MnCl₂, NaCl, EDTA, NP40, Brij 35, glycerol, sucrose, acetic acid, lysozyme, DNAse, RNAse, Phenix imaging film, BSA, Protein G–agarose Ni-resin and liquid scintillant were obtained from Fisher Scientific. γ³²P-ATP was obtained from NEN Products. p-Nitrophenyl Phosphate and 10xPMP buffer (500 mM HEPES, 1 M NaCl, 20 mM DTT, 0.1% Brij 35) were purchased from NEB. Protease inhibitor cocktail was obtained from Roche and anti-PP1γ monoclonal antibody was purchased from Thermo, and anti-GFP monoclonal antibody was purchased from Cell Signaling. InstantBlue was purchased from Expedeon, Hybond ECL nitrocellulose blotting membrane was purchased from Amersham.

ATP, Mops, Tris, MgCl₂, NaCl, EDTA, glycerol, sucrose, acetic acid, lysozyme, DNAse, RNAse, Phenix imaging film, BSA, Protein G–agarose, Ni-resin and liquid scintillant were obtained from Fisher Scientific. ³²P-ATP was obtained from NEN Products. RNA (AGGCGGAGGAAGC) was purchased from Integrated DNA Technologies. siRNA for CLK1 was obtained from Bioneer. Protease inhibitor cocktail was obtained from Roche and TG003 was obtained from Sigma. Anti-CLK1 monoclonal antibody was purchased from Aviva Systems Biology and anti-SRPK1 monoclonal antibody was purchased from BD Biosciences. InstantBlue was purchased from Expedeon, Hybond ECL nitrocellulose blotting membrane was purchased from Amersham and the KinaseMax™ Kit was purchased from Ambion.

Adenosine triphosphate (ATP), 3-(N-morpholino)propanesulphonic acid (MOPS), Tris (hydroxymethyl) aminomethane (Tris), MgCl₂, NaCl, EDTA, acetic acid, Lysozyme, DNAse, RNAse, Phenix imaging film, BSA, and liquid scintillant were obtained from Fisher Scientific. γ³²P-ATP was obtained from NEN Products, a division of Perkin-Elmer Life Sciences. Protease inhibitor cocktail and LysC were obtained from Roche. 10x MnCl₂ and 10x PMP buffer [500 mM HEPES, 100 mM NaCl, 20 mM DTT, 0.1% Brij 35, pH 7.5] was obtained from NEB. The Ron ESE RNA [AGGCGGAGGAAGC] was purchased from Integrated DNA Technologies, Hybond ECL nitrocellulose blotting membrane from Amersham, Bio-Dot microfiltration apparatus from Bio-Rad), KinaseMax^™ Kit from Ambion, and Zip-Tips C₄ from Millipore.

ATP, Mops, HEPES, Tris, MgCl₂, MnCl_2, NaCl, EDTA, Brij 35, glycerol, acetic acid, lysozyme, DNAse, RNAse, Phenix imaging film, BSA, Ni-resin and liquid scintillant were obtained from Fisher Scientific. γ-³²P-ATP was obtained from NEN Products. SRPIN340 was obtained from Sigma. FuGene reagent was obtained from Promeg and Lipofectamine 2000 was obtained from ThermoFisher. Protease inhibitor cocktail, EGF, and TG003 were obtained from Roche. Anti-SRPK1 monoclonal antibody was purchased from BD Biosciences. Anti-CLK1 polyclonal antibody was purchased from Aviva. Anti-SRSF1 monoclonal antibody was purchased from Life Tech. Anti-GFP monoclonal antibody, anti-HA monoclonal antibody, anti-His monoclonal antibody and Protein G beads were purchased from Cell Signaling. Anti-GST monoclonal antibody was purchased from BioLegend. InstantBlue was purchased from Expedeon.