Synaptophysin clone sy38

We used commercial antibodies for the following antigens: N-cadherin (clone 32) and syntaxin-6 (clone 30, BD); β-actin, PSD95, and HA (Sigma); synaptophysin (clone SY38, Abcam); ADAM10 (AB19026), Presenillin1 (MAB5232), GluA1 (MAB2263), and GLT1 (MAB2262, Chemicon/Millipore); GluA2 (#13607), AKT (#9272), phospho-AKT (#9271), and α-Tubulin (#2144, CST); mouse and rabbit IgG, HRP-conjugated (NA931V and NA931934, GE Healthcare).

Hippocampus samples were processed for IFWB analysis as previously described^{57 (link)}. Briefly, samples were adjusted to 0.02 μg for microtubules analysis and 0.2 μg total synaptic markers of total protein concentration per 1 μl with v/v Laemmli buffer 2× (Sigma). Electrophoreses were performed using 26-well 10% Bisacryliamide/Trisacrylamide gels (NuPAGE, Invitrogene) and 7.5 μl of each sample loaded. IFWB was performed with primary antibodies against Acet-Tub (clone 6-11B-1, Sigma) diluted 1:10,000, Total-Tub (clone DM1A, Sigma) diluted 1:15,000, Tyr-Tub (clone TUB-1A2, Sigma) diluted 1:20,000, Glu-Tub (polyclonal, Sigma) diluted 1:4000, Delta2 (polyclonal, Sigma) diluted 1:10,000, PSD-95 (clone 7E3-1B8, Sigma) diluted 1:10,000, Synaptophysin (clone SY38, Abcam) diluted 1:10,000. Membranes were incubated with an anti-mouse IRDye 680 or anti-rabbit IRDye 800 (LiCor) secondary antibody diluted 1:5000. The infrared signal was detected using the Odyssey scanner (LiCor).