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Rabbit anti goat igg hrp sc 2768

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Rabbit anti-goat IgG-HRP (sc-2768) is a secondary antibody conjugated with horseradish peroxidase (HRP). It is designed for the detection of goat primary antibodies in various immunochemical applications.

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Lab products found in correlation

C19 sc 711, by Santa Cruz Biotechnology (2 mentions) Goat polyclonal anti rabbit igg conjugated with hrp sc 2004, by Santa Cruz Biotechnology (2 mentions) Anti akt pan rabbit monoclonal antibody c67e7, by Cell Signaling Technology (2 mentions) Anti ucp 1 pa1 24894, by Thermo Fisher Scientific (2 mentions) Anti phospho erk1 2, by Cell Signaling Technology (2 mentions) Anti erk1 2 9102, by Cell Signaling Technology (2 mentions) Goat anti mouse igg hrp sc 2005, by Santa Cruz Biotechnology (2 mentions) Anti actin antibody, by Merck Group (1 mentions) Mettl14 hpa038002, by Merck Group (1 mentions) Ipvh00010, by Merck Group (1 mentions) Rpn2109, by GE Healthcare (1 mentions) Np0336box, by Thermo Fisher Scientific (1 mentions) Goat anti rabbit igg hrp sc 2004, by Santa Cruz Biotechnology (1 mentions) Anti cleaved caspase 3 9664, by Cell Signaling Technology (1 mentions) Mouse anti gfap, by BD (1 mentions) Anti rip1 3493, by Cell Signaling Technology (1 mentions) Anti mouse alexa 488, by Vector Laboratories (1 mentions) Anti rabbit alexa 594, by Vector Laboratories (1 mentions) Mouse anti β actin, by Cell Signaling Technology (1 mentions) Trypsin, by Thermo Fisher Scientific (1 mentions)

5 protocols using rabbit anti goat igg hrp sc 2768

1

Antibody Immunoblotting for Insulin Signaling

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The rabbit polyclonal anti-insulin receptor (IR) β-subunit (C19; sc-711), the mouse monoclonal anti-phosphotyrosine (PY99; sc-7020), the goat polyclonal anti-rabbit IgG conjugated with HRP (sc-2004), the goat anti-mouse IgG-HRP (sc-2005) and rabbit anti-goat IgG-HRP (sc-2768) antibodies were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). The rabbit polyclonal antibody anti-phospho-Akt-S473 (4060), the rabbit polyclonal antibody anti-phospho-Akt-T308 (9275), the anti-Akt (pan) rabbit monoclonal antibody (C67E7) that detects endogenous levels of total AKT1, AKT2, and AKT3 protein, the rabbit monoclonal, the rabbit monoclonal antibody anti-phospho-ERK1/2, that detects ERK-1 and −2 (p44 and p42 MAPK respectively) when phosphorylated at T202 and Y204 (4370) and the rabbit polyclonal antibody anti-ERK1/2 (9102) were from Cell Signalling (Danvers, MA, USA). The goat polyclonal anti-adiponectin (AF1119) antibody was from R&D systems (Minneapolis, MN, USA). The rabbit polyclonal antibody anti-UCP-1 (PA1–24894 was from Thermo Fisher (Rockford, IL, USA). Protein loading in the gels was evaluated with a polyclonal anti-actin antibody produced in rabbit (Sigma-Aldrich, USA, Catalog Number A2066).
Quiroga D.T., Miquet J.G., Gonzalez L., Sotelo A.I., Muñoz M.C., Geraldes P.M., Giani J.F, & Dominici F.P. (2019). Mice lacking angiotensin type 2 receptor exhibit a sex-specific attenuation of insulin sensitivity. Molecular and cellular endocrinology, 498, 110587.
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2

Western Blot Analysis of RNA Methylation Proteins

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Western blots were performed using standard procedures. Briefly, 10–30 μg protein samples were separated on 4–12% polyacrylamide Bis-Tris gels (NP0336BOX, Invitrogen) and transferred to polyvinylidene fluoride membranes (IPVH00010, Millipore). The blots were probed with METTL3- (15073-1-AP, Proteintech), METTL14- (HPA038002, Sigma), HNRNPG- (sc-14581 and sc-48796, Santa Cruz Biotechnology) or GAPDH- (A00192-40, Genscript) specific primary antibody, followed by rabbit anti-goat IgG-HRP (sc-2768, Santa Cruz Biotechnology) or goat anti-rabbit IgG-HRP (ab97051, Abcam) secondary antibody, and then visualized by enhanced chemoluminescence (RPN2109, GE Healthcare).
Liu N., Zhou K.I., Parisien M., Dai Q., Diatchenko L, & Pan T. (2017). N 6-methyladenosine alters RNA structure to regulate binding of a low-complexity protein. Nucleic Acids Research, 45(10), 6051-6063.
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3

Antibody Acquisition for Research

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Anti-RPE65 (ab231782), anti-RIP3 (phosphor s232, ab195117), and anti-Rodopsin (ab5417) were purchased from abcam (Boston, MA, USA). Other antibodies used in this study were obtained from the following sources: anti-cleaved caspase 3 (#9664), anti-RIP1 (#3493), and anti-mouse β-actin (#3700) were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA). Mouse anti-GFAP (glial fibrillary acidic protein, specific for glia/Müller cells) was obtained from BD Biosciences (San Jose, CA, USA). Anti-mouse Iba1 was purchased from FUJIFILM WAKO Chemicals, U.S.A. Corporation (019-19741, Richmond, VA, USA). Goat anti-rabbit IgG HRP (sc2004) and rabbit anti-goat IgG HRP (sc2768) were sourced from Santa Cruz (Santa Cruz Biotechnology, CA, USA). Anti-Mouse IgG HRP Conjugate (W402B) was obtained from Promega (Madison WI, USA). Anti-mouse Alexa 488 and anti-rabbit Alexa 594 were obtained from Vector Laboratories, Inc. (Burlingame, CA, USA).
Zhang X., Xu J., Marshall B., Dong Z., Liu Y., Espinosa-Heidmann D.G, & Zhang M. (2023). Transcriptome Analysis of Retinal and Choroidal Pathologies in Aged BALB/c Mice Following Systemic Neonatal Murine Cytomegalovirus Infection. International Journal of Molecular Sciences, 24(5), 4322.
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4

Comprehensive Cell Culture Protocol

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Fetal bovine serum (HyClone, Logan, Utah, USA), trypsin (Gibco, Grand Island, NY, USA), α-modified Eagle's medium (MEM) (Life Technologies Corporation, NY, USA, lot number 1406320), 2x Taq PCR Master Mix (dye included), Taq polymerase, SYBR Green PCR Master Mix (Invitrogen Corporation, Carlsbad, CA, USA), DEPC (diethyl pyrocarbonate), DEPC-treated water (DNase/RNase-free double distilled H2O) (Amresco, Solon, OH, USA), anti-rabbit IgG HRP-linked (7074S, Cell Signaling Technology, Danvers, MA, USA), rabbit anti-goat IgG-HRP (sc-2768, Santa Cruz, USA), and Western blot marker (color predyed) (Thermo Fisher Scientific) were used.
Wu R., Li Q., Pei X, & Hu K. (2017). Effects of Brucine on the OPG/RANKL/RANK Signaling Pathway in MDA-MB-231 and MC3T3-E1 Cell Coculture System. Evidence-based Complementary and Alternative Medicine : eCAM, 2017, 1693643.
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5

Antibodies for Molecular Signaling Analysis

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The rabbit polyclonal antiinsulin receptor (IR) β‐subunit (C19; sc‐711), the mouse monoclonal antiphosphotyrosine (PY99; sc‐7020), the goat polyclonal anti‐rabbit IgG conjugated with HRP (sc‐2004), the goat anti‐mouse IgG‐HRP (sc‐2005), and rabbit anti‐goat IgG‐HRP (sc‐2768) antibodies were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). The rabbit polyclonal antibody antiphospho‐Akt‐S473 (4060), the rabbit polyclonal antibody antiphospho‐Akt‐T308 (9275), the anti‐Akt (pan) rabbit monoclonal antibody (C67E7) that detects endogenous levels of total AKT1, AKT2, and AKT3 protein, the rabbit monoclonal, the rabbit monoclonal antibody antiphospho‐ERK1/2, that detects ERK‐1 and ‐2 (p44 and p42 MAPK, respectively) when phosphorylated at T202 and Y204 (4370) and the rabbit polyclonal antibody anti‐ERK1/2 (9102) were from Cell Signalling (Danvers, MA). The goat polyclonal anti‐mouse TNF‐α (MAB4101) and antiadiponectin (AF1119) antibodies were from R&D systems (Minneapolis, MN). The rabbit polyclonal antibody antinitrotyrosine (AB5411) was from Millipore Sigma (St. Louis, MO). The rabbit polyclonal antibody anti‐UCP‐1 (PA1‐24894 was from Thermo Fisher (Rockford, IL). Protein loading in the gels was evaluated with a mouse monoclonal anti‐GADPDH (ab9485) from Abcam (San Francisco, CA).
Quiroga D.T., Muñoz M.C., Gil C., Pffeifer M., Toblli J.E., Steckelings U.M., Giani J.F, & Dominici F.P. (2018). Chronic administration of the angiotensin type 2 receptor agonist C21 improves insulin sensitivity in C57BL/6 mice.. Physiological Reports, 6(16), e13824.
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