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Ultra sensitive autoradiography film

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Ultra-sensitive autoradiography film is a type of film used in the detection and visualization of radioactive signals in biological and biochemical experiments. The film is highly sensitive to low levels of radiation, allowing researchers to capture and record the presence and distribution of radioactive labels in their samples.

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Lab products found in correlation

Laemmli buffer, by Merck Group (2 mentions) Total h3, by Abcam (2 mentions) H3k27me3, by Thermo Fisher Scientific (2 mentions) Nupage 4 12 bis tris protein gel, by Thermo Fisher Scientific (2 mentions) Nitrocellulose membrane, by Bio-Rad (2 mentions) Dc protein assay, by Bio-Rad (2 mentions)

Close spelling variants of this product

Autoradiography film

2 protocols using ultra sensitive autoradiography film

1

Western Blot Analysis of Histone Modifications

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells or tumor tissues were lysed in Laemmli buffer (Sigma) and cleared. Protein concentration was determined using DC protein assay (Bio-rad). Proteins (25 μg) were separated on precast NuPAGE 4–12% Bis-Tris protein gels (ThermoFisher), and transferred onto nitrocellulose membranes (Bio-Rad). After blocking with 5% BSA buffer for 1 hour at room temperature, membranes were incubated with primary antibodies overnight at the recommended concentrations. HRP conjugated secondary antibodies (1:10,000; Bio-rad; Cat#5196–2504) were applied, and ultra-sensitive autoradiography film (Amersham) was used to detect the chemiluminescence signal. Primary antibodies used are H3K27me3 (1:1,000, Invitrogen Cat#MA5–11198) and H3 total (1:1,000, Abcam Cat#1791).
Guo H., Vuille J.A., Wittner B.S., Lachtara E.M., Hou Y., Lin M., Zhao T., Raman A.T., Russell H.C., Reeves B.A., Pleskow H.M., Wu C.L., Gnirke A., Meissner A., Efstathiou J.A., Lee R.J., Toner M., Aryee M.J., Lawrence M.S., Miyamoto D.T., Maheswaran S, & Haber D.A. (2023). DNA hypomethylation silences antitumor immune genes in early prostate cancer and CTCs. Cell, 186(13), 2765-2782.e28.
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2

Western Blot Analysis of Histone Modifications

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells or tumor tissues were lysed in Laemmli buffer (Sigma) and cleared. Protein concentration was determined using DC protein assay (Bio-rad). Proteins (25 μg) were separated on precast NuPAGE 4–12% Bis-Tris protein gels (ThermoFisher), and transferred onto nitrocellulose membranes (Bio-Rad). After blocking with 5% BSA buffer for 1 hour at room temperature, membranes were incubated with primary antibodies overnight at the recommended concentrations. HRP conjugated secondary antibodies (1:10,000; Bio-rad; Cat#5196–2504) were applied, and ultra-sensitive autoradiography film (Amersham) was used to detect the chemiluminescence signal. Primary antibodies used are H3K27me3 (1:1,000, Invitrogen Cat#MA5–11198) and H3 total (1:1,000, Abcam Cat#1791).
Guo H., Vuille J.A., Wittner B.S., Lachtara E.M., Hou Y., Lin M., Zhao T., Raman A.T., Russell H.C., Reeves B.A., Pleskow H.M., Wu C.L., Gnirke A., Meissner A., Efstathiou J.A., Lee R.J., Toner M., Aryee M.J., Lawrence M.S., Miyamoto D.T., Maheswaran S, & Haber D.A. (2023). DNA hypomethylation silences antitumor immune genes in early prostate cancer and CTCs. Cell, 186(13), 2765-2782.e28.
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