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4 protocols using ketorolac tromethamine

1

Formulation and Characterization of Topical Ketorolac Gel

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Sodium alginate was purchased from Fagron Iberica (Terrassa, Spain). Ketorolac tromethamine and Nipagin were obtained from Sigma-Aldrich (Barcelona, Spain). Nipasol was acquired from Acofarma (Barcelona, Spain), and Na2HPO4 and KH2PO4 were supplied by Panreac (Barcelona, Spain). PBS pH 7.6, gentamicin sulphate, and bovine serum albumin were provided by Sigma (St. Louis, MO, USA); methanol was obtained from Merck, Darmstadt, Germany. The adhesive tapes were obtained from D-squame, (Cuderm Co., Dallas, TX, USA).
The purified water was obtained from a Milli-Q1 Gradinet A10 system apparatus (Millipore Iberica S.A.U., Madrid, Spain). All the other chemicals and reagents used in the study were of analytical grade.
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2

Biopolymer-based Drug Delivery System

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Sodium alginate was purchased from Fagron Iberica (Terrassa, Spain). Ketorolac tromethamine and Nipagin were obtained from Sigma-Aldrich (Barcelona, Spain). Nipasol was acquired from Acofarma (Barcelona, Spain), Hyaluronic acids were obtained from Fagron Iberica (Terrassa, Spain); Na2HPO4 and KH2PO4 were supplied by Panreac (Barcelona, Spain), NaCl and KCl were from Merck (Darmstadt, Germany), CaCl from Ferosa (Spain) Hepes was obtained from Fagron Iberica (Terrassa, Spain) and glucose from Sigma-Aldrich (Barcelona, Spain). The Millipore Express® PLUS 0.45 µm PES Membrane was from Merck (Darmstadt, Germany), the Nylon membrane Filter with 0.45 µm pore size from Teknokroma (Barcelona, Spain) and the biomimetic membrane PermeaPad® from InnoME GmbH (Espelkamp, Germany).
The purified water was obtained from a Milli-Q1 Gradient A10 system apparatus (Millipore Iberica S.A.U., Madrid, Spain). All the other chemicals and reagents used in the study were of analytical grade.
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3

Development of Alginate-Based Bioactive Formulations

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Sodium alginate was purchased from Fagron Iberica (Barcelona, Spain). Calcium chloride (CaCl2), ketorolac tromethamine (KT), and nipagin were purchased from Sigma-Aldrich (Barcelona, Spain). Nipasol from Acofarma (Barcelona, Spain) and Na2HPO4, KH2PO4, and NaCl by Panreac (Barcelona, Spain). Methanol from Merck (Darmstadt, Germany). The purified water was obtained from a Milli-Q1 Gradient A10 system apparatus (Millipore Iberica S.A.U. Madrid, Spain). All the other chemicals and reagents used in the study were of analytical grade.
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4

Mucoadhesive Chitosan-based Ketorolac Delivery

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Ketorolac tromethamine (KT), low molecular weight chitosan (deacetylation degree 85%, viscosity 20,000 cP), sodium tripolyphosphate (TPP) and porcine mucin were purchased from Sigma Aldrich chemicals Co., UK. Glacial acetic acid was purchased from Fisher Scientific, UK. All other solvents and buffer salts were obtained from Sigma Aldrich chemical Co., UK; were of analytical grade and used as received.
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